##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI AviII
BamHI BbeI BbrPI BcgI BciVI
BfrI BglI BglII BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BsrDI BssHII BstBI ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI FseI FspI HpaI
KasI KspI MamI MluI MroI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NspV PacI PinAI
PmaCI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SmaI SpeI SphI Sse8387I StuI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 593 CGCG CG^CG
AcyI 729 GACGCC G[GA]^CG[CT]C
AflIII 3741 ACACGT A^C[GA][CT]GT
Alw44I 627 GTGCAC G^TGCAC
AocI 2336 CCTCAGG CC^T[ACGT]AGG
ApaLI 627 GTGCAC G^TGCAC
Asp700I 913 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 573 CTCGAG C^[CT]CG[GA]G
BclI 3116 TGATCA T^GATCA
BlpI 237 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 237 GCTCAGC GC^T[ACGT]AGC
BsaHI 729 GACGCC G[GA]^CG[CT]C
BsmBI 1250 GAGACG CGTCTC(1/5)
BsoBI 573 CTCGAG C^[CT]CG[GA]G
Bsp1407I 128 TGTACA T^GTACA
BsrBI 871 CCGCTC CCGCTC(-3/-3)
BsrGI 128 TGTACA T^GTACA
Bst1107I 4735 GTATAC GTA^TAC
BstEII 1346 GGTTACC G^GT[ACGT]ACC
BstPI 1346 GGTTACC G^GT[ACGT]ACC
BstUI 593 CGCG CG^CG
BstZ17I 4735 GTATAC GTA^TAC
Bsu36I 2336 CCTCAGG CC^T[ACGT]AGG
CelII 237 GCTCAGC GC^T[ACGT]AGC
Eco81I 2336 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1346 GGTTACC G^GT[ACGT]ACC
EcoRV 2600 GATATC GAT^ATC
EcoT22I 4242 ATGCAT ATGCA^T
FauI 3100 CCCGC CCCGC(4/6)
FbaI 3116 TGATCA T^GATCA
HaeII 90 AGCGCC [GA]GCGC^[CT]
HgaI 729 GACGC GACGC(5/10)
Hin1I 729 GACGCC G[GA]^CG[CT]C
HincII 1319 GTCAAC GT[CT]^[GA]AC
HindII 1319 GTCAAC GT[CT]^[GA]AC
HindIII 4328 AAGCTT A^AGCTT
MvnI 593 CGCG CG^CG
NsiI 4242 ATGCAT ATGCA^T
PaeR7I 573 CTCGAG C^TCGAG
PflMI 1650 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 1714 GTTTAAAC GTTT^AAAC
SacI 213 GAGCTC GAGCT^C
SgrAI 5 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 3517 TACGTA TAC^GTA
SspBI 128 TGTACA T^GTACA
SwaI 3613 ATTTAAAT ATTT^AAAT
Van91I 1650 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4554 TCTAGA T^CTAGA
XhoI 573 CTCGAG C^TCGAG
XmnI 913 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 380 GGTACC G^GTACC
738 GGTACC
AccI 4434 GTAGAC GT^[AC][GT]AC
4735 GTATAC
Asp718I 380 GGTACC G^GTACC
738 GGTACC
AspI 3961 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4564 GACTATGTC
AvrII 2008 CCTAGG C^CTAGG
2644 CCTAGG
BbvCI 2194 CCTCAGC CCTCAGC(-5/-2)
1770 GCTGAGG
BcnI 644 CCCGG CC^[GC]GG
723 CCGGG
BlnI 2008 CCTAGG C^CTAGG
2644 CCTAGG
BsaAI 3517 TACGTA [CT]AC^GT[GA]
3742 CACGTA
BsmI 2262 GCATTC GAATGC(1/-1)
4305 GCATTC
BsrFI 6 GCCGGT [GA]^CCGG[CT]
419 ACCGGC
BstAPI 684 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2993 GCAAATCTTGC
Cfr10I 6 GCCGGT [GA]^CCGG[CT]
419 ACCGGC
DraIII 721 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4317 CACAGGGTG
KpnI 380 GGTACC GGTAC^C
738 GGTACC
MfeI 1182 CAATTG C^AATTG
4740 CAATTG
MspA1I 13 CCGCTG C[AC]G^C[GT]G
94 CCGCTG
MunI 1182 CAATTG C^AATTG
4740 CAATTG
NciI 644 CCCGG CC^[GC]GG
723 CCGGG
NheI 2225 GCTAGC G^CTAGC
3760 GCTAGC
PflFI 3961 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4564 GACTATGTC
Tth111I 3961 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4564 GACTATGTC
XcmI 608 CCAGGAAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4045 CCAAAGTTCACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApaI ApoI AspEI
AspHI AvaII BalI BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eam1105I
EarI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RcaI RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII