################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI AviII BamHI BbeI BbrPI BcgI BciVI BfrI BglI BglII BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrDI BssHII BstBI ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoNI FseI FspI HpaI KasI KspI MamI MluI MroI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SmaI SpeI SphI Sse8387I StuI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 593 CGCG CG^CG AcyI 729 GACGCC G[GA]^CG[CT]C AflIII 3741 ACACGT A^C[GA][CT]GT Alw44I 627 GTGCAC G^TGCAC AocI 2336 CCTCAGG CC^T[ACGT]AGG ApaLI 627 GTGCAC G^TGCAC Asp700I 913 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 573 CTCGAG C^[CT]CG[GA]G BclI 3116 TGATCA T^GATCA BlpI 237 GCTCAGC GC^T[ACGT]AGC Bpu1102I 237 GCTCAGC GC^T[ACGT]AGC BsaHI 729 GACGCC G[GA]^CG[CT]C BsmBI 1250 GAGACG CGTCTC(1/5) BsoBI 573 CTCGAG C^[CT]CG[GA]G Bsp1407I 128 TGTACA T^GTACA BsrBI 871 CCGCTC CCGCTC(-3/-3) BsrGI 128 TGTACA T^GTACA Bst1107I 4735 GTATAC GTA^TAC BstEII 1346 GGTTACC G^GT[ACGT]ACC BstPI 1346 GGTTACC G^GT[ACGT]ACC BstUI 593 CGCG CG^CG BstZ17I 4735 GTATAC GTA^TAC Bsu36I 2336 CCTCAGG CC^T[ACGT]AGG CelII 237 GCTCAGC GC^T[ACGT]AGC Eco81I 2336 CCTCAGG CC^T[ACGT]AGG EcoO65I 1346 GGTTACC G^GT[ACGT]ACC EcoRV 2600 GATATC GAT^ATC EcoT22I 4242 ATGCAT ATGCA^T FauI 3100 CCCGC CCCGC(4/6) FbaI 3116 TGATCA T^GATCA HaeII 90 AGCGCC [GA]GCGC^[CT] HgaI 729 GACGC GACGC(5/10) Hin1I 729 GACGCC G[GA]^CG[CT]C HincII 1319 GTCAAC GT[CT]^[GA]AC HindII 1319 GTCAAC GT[CT]^[GA]AC HindIII 4328 AAGCTT A^AGCTT MvnI 593 CGCG CG^CG NsiI 4242 ATGCAT ATGCA^T PaeR7I 573 CTCGAG C^TCGAG PflMI 1650 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 1714 GTTTAAAC GTTT^AAAC SacI 213 GAGCTC GAGCT^C SgrAI 5 CGCCGGTG C[GA]^CCGG[CT]G SnaBI 3517 TACGTA TAC^GTA SspBI 128 TGTACA T^GTACA SwaI 3613 ATTTAAAT ATTT^AAAT Van91I 1650 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4554 TCTAGA T^CTAGA XhoI 573 CTCGAG C^TCGAG XmnI 913 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Acc65I 380 GGTACC G^GTACC 738 GGTACC AccI 4434 GTAGAC GT^[AC][GT]AC 4735 GTATAC Asp718I 380 GGTACC G^GTACC 738 GGTACC AspI 3961 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4564 GACTATGTC AvrII 2008 CCTAGG C^CTAGG 2644 CCTAGG BbvCI 2194 CCTCAGC CCTCAGC(-5/-2) 1770 GCTGAGG BcnI 644 CCCGG CC^[GC]GG 723 CCGGG BlnI 2008 CCTAGG C^CTAGG 2644 CCTAGG BsaAI 3517 TACGTA [CT]AC^GT[GA] 3742 CACGTA BsmI 2262 GCATTC GAATGC(1/-1) 4305 GCATTC BsrFI 6 GCCGGT [GA]^CCGG[CT] 419 ACCGGC BstAPI 684 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2993 GCAAATCTTGC Cfr10I 6 GCCGGT [GA]^CCGG[CT] 419 ACCGGC DraIII 721 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4317 CACAGGGTG KpnI 380 GGTACC GGTAC^C 738 GGTACC MfeI 1182 CAATTG C^AATTG 4740 CAATTG MspA1I 13 CCGCTG C[AC]G^C[GT]G 94 CCGCTG MunI 1182 CAATTG C^AATTG 4740 CAATTG NciI 644 CCCGG CC^[GC]GG 723 CCGGG NheI 2225 GCTAGC G^CTAGC 3760 GCTAGC PflFI 3961 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4564 GACTATGTC Tth111I 3961 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4564 GACTATGTC XcmI 608 CCAGGAAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4045 CCAAAGTTCACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RcaI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII