##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
Aor51HI     AscI        AseI        AsnI        AviII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BfrI        BglI        BglII       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BspLU11I    BsrDI       BssHII      BstBI       ClaI        
CpoI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       FseI        FspI        HpaI        
KasI        KspI        MamI        MluI        MroI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SmaI        SpeI        SphI        Sse8387I    StuI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII         593  CGCG                 CG^CG
AcyI          729  GACGCC               G[GA]^CG[CT]C
AflIII       3741  ACACGT               A^C[GA][CT]GT
Alw44I        627  GTGCAC               G^TGCAC
AocI         2336  CCTCAGG              CC^T[ACGT]AGG
ApaLI         627  GTGCAC               G^TGCAC
Asp700I       913  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          573  CTCGAG               C^[CT]CG[GA]G
BclI         3116  TGATCA               T^GATCA
BlpI          237  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      237  GCTCAGC              GC^T[ACGT]AGC
BsaHI         729  GACGCC               G[GA]^CG[CT]C
BsmBI        1250  GAGACG               CGTCTC(1/5)
BsoBI         573  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      128  TGTACA               T^GTACA
BsrBI         871  CCGCTC               CCGCTC(-3/-3)
BsrGI         128  TGTACA               T^GTACA
Bst1107I     4735  GTATAC               GTA^TAC
BstEII       1346  GGTTACC              G^GT[ACGT]ACC
BstPI        1346  GGTTACC              G^GT[ACGT]ACC
BstUI         593  CGCG                 CG^CG
BstZ17I      4735  GTATAC               GTA^TAC
Bsu36I       2336  CCTCAGG              CC^T[ACGT]AGG
CelII         237  GCTCAGC              GC^T[ACGT]AGC
Eco81I       2336  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1346  GGTTACC              G^GT[ACGT]ACC
EcoRV        2600  GATATC               GAT^ATC
EcoT22I      4242  ATGCAT               ATGCA^T
FauI         3100  CCCGC                CCCGC(4/6)
FbaI         3116  TGATCA               T^GATCA
HaeII          90  AGCGCC               [GA]GCGC^[CT]
HgaI          729  GACGC                GACGC(5/10)
Hin1I         729  GACGCC               G[GA]^CG[CT]C
HincII       1319  GTCAAC               GT[CT]^[GA]AC
HindII       1319  GTCAAC               GT[CT]^[GA]AC
HindIII      4328  AAGCTT               A^AGCTT
MvnI          593  CGCG                 CG^CG
NsiI         4242  ATGCAT               ATGCA^T
PaeR7I        573  CTCGAG               C^TCGAG
PflMI        1650  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI         1714  GTTTAAAC             GTTT^AAAC
SacI          213  GAGCTC               GAGCT^C
SgrAI           5  CGCCGGTG             C[GA]^CCGG[CT]G
SnaBI        3517  TACGTA               TAC^GTA
SspBI         128  TGTACA               T^GTACA
SwaI         3613  ATTTAAAT             ATTT^AAAT
Van91I       1650  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         4554  TCTAGA               T^CTAGA
XhoI          573  CTCGAG               C^TCGAG
XmnI          913  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Acc65I        380  GGTACC               G^GTACC
              738  GGTACC              
AccI         4434  GTAGAC               GT^[AC][GT]AC
             4735  GTATAC              
Asp718I       380  GGTACC               G^GTACC
              738  GGTACC              
AspI         3961  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4564  GACTATGTC           
AvrII        2008  CCTAGG               C^CTAGG
             2644  CCTAGG              
BbvCI        2194  CCTCAGC              CCTCAGC(-5/-2)
             1770  GCTGAGG             
BcnI          644  CCCGG                CC^[GC]GG
              723  CCGGG               
BlnI         2008  CCTAGG               C^CTAGG
             2644  CCTAGG              
BsaAI        3517  TACGTA               [CT]AC^GT[GA]
             3742  CACGTA              
BsmI         2262  GCATTC               GAATGC(1/-1)
             4305  GCATTC              
BsrFI           6  GCCGGT               [GA]^CCGG[CT]
              419  ACCGGC              
BstAPI        684  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2993  GCAAATCTTGC         
Cfr10I          6  GCCGGT               [GA]^CCGG[CT]
              419  ACCGGC              
DraIII        721  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4317  CACAGGGTG           
KpnI          380  GGTACC               GGTAC^C
              738  GGTACC              
MfeI         1182  CAATTG               C^AATTG
             4740  CAATTG              
MspA1I         13  CCGCTG               C[AC]G^C[GT]G
               94  CCGCTG              
MunI         1182  CAATTG               C^AATTG
             4740  CAATTG              
NciI          644  CCCGG                CC^[GC]GG
              723  CCGGG               
NheI         2225  GCTAGC               G^CTAGC
             3760  GCTAGC              
PflFI        3961  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4564  GACTATGTC           
Tth111I      3961  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4564  GACTATGTC           
XcmI          608  CCAGGAAGAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4045  CCAAAGTTCACTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AcsI        AfaI        AhdI        AluI        
AlwI        AlwNI       ApaI        ApoI        AspEI       
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        Eam1105I    
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII