##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BamHI BbeI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BsmBI BsmI BspMI
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MscI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1788 GTCTAC GT^[AC][GT]AC
AccIII 258 TCCGGA T^CCGGA
AcyI 1308 GACGCC G[GA]^CG[CT]C
AhdI 2407 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2519 GTGCAC G^TGCAC
ApaLI 2519 GTGCAC G^TGCAC
Asp700I 554 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2407 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1154 GGCACC G^G[CT][GA]CC
BbrPI 1149 CACGTG CAC^GTG
BsaAI 1149 CACGTG [CT]AC^GT[GA]
BsaHI 1308 GACGCC G[GA]^CG[CT]C
BseAI 258 TCCGGA T^CCGGA
BsiEI 72 CGACCG CG[GA][CT]^CG
Bsp1407I 211 TGTACA T^GTACA
BspDI 306 ATCGAT AT^CGAT
BspEI 258 TCCGGA T^CCGGA
BspHI 979 TCATGA T^CATGA
BspLU11I 208 ACATGT A^CATGT
BsrGI 211 TGTACA T^GTACA
BstAPI 722 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 306 ATCGAT AT^CGAT
DraIII 3400 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2717 TGGCCG [CT]^GGCC[GA]
Eam1105I 2407 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2196 GATATC GAT^ATC
HaeII 2118 AGCGCC [GA]GCGC^[CT]
Hin1I 1308 GACGCC G[GA]^CG[CT]C
HincII 2590 GTTGAC GT[CT]^[GA]AC
HindII 2590 GTTGAC GT[CT]^[GA]AC
KspI 2814 CCGCGG CCGC^GG
MroI 258 TCCGGA T^CCGGA
NaeI 18 GCCGGC GCC^GGC
NgoMIV 18 GCCGGC G^CCGGC
NheI 1235 GCTAGC G^CTAGC
PaeR7I 1717 CTCGAG C^TCGAG
PmaCI 1149 CACGTG CAC^GTG
PmlI 1149 CACGTG CAC^GTG
PpuMI 1264 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 979 TCATGA T^CATGA
SacII 2814 CCGCGG CCGC^GG
SexAI 2799 ACCAGGT A^CC[AT]GGT
SfiI 17 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2848 GCATGC GCATG^C
SspBI 211 TGTACA T^GTACA
StuI 1102 AGGCCT AGG^CCT
XbaI 1247 TCTAGA T^CTAGA
XhoI 1717 CTCGAG C^TCGAG
XmnI 554 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 1275 CGCG CG^CG
2815 CGCG
AflIII 208 ACATGT A^C[GA][CT]GT
1750 ACACGT
BbsI 92 GAAGAC GAAGAC(2/6)
1515 GTCTTC
BciVI 1388 GGATAC GTATCC(6/5)
2688 GTATCC
BglI 18 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
27 GCCGGGGCGGC
BpuAI 92 GAAGAC GAAGAC(2/6)
1515 GTCTTC
BsaI 84 GAGACC GGTCTC(1/5)
2335 GAGACC
BseRI 2226 CTCCTC GAGGAG(10/8)
2532 GAGGAG
BsrFI 18 GCCGGC [GA]^CCGG[CT]
160 GCCGGT
BstUI 1275 CGCG CG^CG
2815 CGCG
CfoI 138 GCGC GCG^C
2119 GCGC
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
160 GCCGGT
DraII 1264 AGGACCT [GA]G^G[ACGT]CC[CT]
1983 GGGGCCT
EcoO109I 1264 AGGACCT [GA]G^G[ACGT]CC[CT]
1983 GGGGCCT
HgaI 1172 GACGC GACGC(5/10)
1308 GACGC
HhaI 138 GCGC GCG^C
2119 GCGC
HinP1I 138 GCGC G^CGC
2119 GCGC
MvnI 1275 CGCG CG^CG
2815 CGCG
PvuII 200 CAGCTG CAG^CTG
2831 CAGCTG
SapI 557 GCTCTTC GCTCTTC(1/4)
2319 GCTCTTC
ScaI 1027 AGTACT AGT^ACT
2572 AGTACT
SmlI 1717 CTCGAG C^T[CT][GA]AG
2443 CTCAAG
TfiI 1374 GATTC G^A[AT]TC
3135 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII