##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
BamHI BbeI BbrPI BcgI BfrI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoRI FseI
FspI HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 1412 ACATGT A^C[GA][CT]GT
Alw44I 4009 GTGCAC G^TGCAC
ApaLI 4009 GTGCAC G^TGCAC
AspI 1944 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3506 CCTAGG C^CTAGG
BciVI 3744 GGATAC GTATCC(6/5)
BclI 911 TGATCA T^GATCA
BglII 117 AGATCT A^GATCT
BlnI 3506 CCTAGG C^CTAGG
BsmBI 814 CGTCTC CGTCTC(1/5)
BspLU11I 1412 ACATGT A^CATGT
BsrFI 899 GCCGGC [GA]^CCGG[CT]
BstEII 2867 GGTAACC G^GT[ACGT]ACC
BstPI 2867 GGTAACC G^GT[ACGT]ACC
Cfr10I 899 GCCGGC [GA]^CCGG[CT]
EcoO65I 2867 GGTAACC G^GT[ACGT]ACC
EcoT22I 2240 ATGCAT ATGCA^T
FbaI 911 TGATCA T^GATCA
HincII 2389 GTCAAC GT[CT]^[GA]AC
HindII 2389 GTCAAC GT[CT]^[GA]AC
HindIII 3001 AAGCTT A^AGCTT
NaeI 899 GCCGGC GCC^GGC
NgoMIV 899 GCCGGC G^CCGGC
NruI 314 TCGCGA TCG^CGA
NsiI 2240 ATGCAT ATGCA^T
PflFI 1944 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2037 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 4144 GCTCTTC GCTCTTC(1/4)
SbfI 4041 CCTGCAGG CCTGCA^GG
ScaI 2280 AGTACT AGT^ACT
SphI 788 GCATGC GCATG^C
Sse8387I 4041 CCTGCAGG CCTGCA^GG
StuI 2419 AGGCCT AGG^CCT
Tth111I 1944 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 315 CGCG CG^CG
1647 CGCG
AcyI 299 GGCGTC G[GA]^CG[CT]C
1060 GGCGTC
BalI 158 TGGCCA TGG^CCA
640 TGGCCA
BanI 243 GGTGCC G^G[CT][GA]CC
1982 GGTGCC
BsaHI 299 GGCGTC G[GA]^CG[CT]C
1060 GGCGTC
BsmI 2403 GAATGC GAATGC(1/-1)
1635 GCATTC
BstUI 315 CGCG CG^CG
1647 CGCG
DraIII 3434 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4012 CACTGTGTG
EaeI 158 TGGCCA [CT]^GGCC[GA]
640 TGGCCA
Hin1I 299 GGCGTC G[GA]^CG[CT]C
1060 GGCGTC
MluNI 158 TGGCCA TGG^CCA
640 TGGCCA
MscI 158 TGGCCA TGG^CCA
640 TGGCCA
MvnI 315 CGCG CG^CG
1647 CGCG
Tsp45I 517 GTGAC ^GT[GC]AC
2147 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI Aor51HI ApaI ApoI
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI