################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AviII BamHI BbeI BbrPI BcgI BfrI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRI FseI FspI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflIII 1412 ACATGT A^C[GA][CT]GT Alw44I 4009 GTGCAC G^TGCAC ApaLI 4009 GTGCAC G^TGCAC AspI 1944 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3506 CCTAGG C^CTAGG BciVI 3744 GGATAC GTATCC(6/5) BclI 911 TGATCA T^GATCA BglII 117 AGATCT A^GATCT BlnI 3506 CCTAGG C^CTAGG BsmBI 814 CGTCTC CGTCTC(1/5) BspLU11I 1412 ACATGT A^CATGT BsrFI 899 GCCGGC [GA]^CCGG[CT] BstEII 2867 GGTAACC G^GT[ACGT]ACC BstPI 2867 GGTAACC G^GT[ACGT]ACC Cfr10I 899 GCCGGC [GA]^CCGG[CT] EcoO65I 2867 GGTAACC G^GT[ACGT]ACC EcoT22I 2240 ATGCAT ATGCA^T FbaI 911 TGATCA T^GATCA HincII 2389 GTCAAC GT[CT]^[GA]AC HindII 2389 GTCAAC GT[CT]^[GA]AC HindIII 3001 AAGCTT A^AGCTT NaeI 899 GCCGGC GCC^GGC NgoMIV 899 GCCGGC G^CCGGC NruI 314 TCGCGA TCG^CGA NsiI 2240 ATGCAT ATGCA^T PflFI 1944 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2037 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 4144 GCTCTTC GCTCTTC(1/4) SbfI 4041 CCTGCAGG CCTGCA^GG ScaI 2280 AGTACT AGT^ACT SphI 788 GCATGC GCATG^C Sse8387I 4041 CCTGCAGG CCTGCA^GG StuI 2419 AGGCCT AGG^CCT Tth111I 1944 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccII 315 CGCG CG^CG 1647 CGCG AcyI 299 GGCGTC G[GA]^CG[CT]C 1060 GGCGTC BalI 158 TGGCCA TGG^CCA 640 TGGCCA BanI 243 GGTGCC G^G[CT][GA]CC 1982 GGTGCC BsaHI 299 GGCGTC G[GA]^CG[CT]C 1060 GGCGTC BsmI 2403 GAATGC GAATGC(1/-1) 1635 GCATTC BstUI 315 CGCG CG^CG 1647 CGCG DraIII 3434 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4012 CACTGTGTG EaeI 158 TGGCCA [CT]^GGCC[GA] 640 TGGCCA Hin1I 299 GGCGTC G[GA]^CG[CT]C 1060 GGCGTC MluNI 158 TGGCCA TGG^CCA 640 TGGCCA MscI 158 TGGCCA TGG^CCA 640 TGGCCA MvnI 315 CGCG CG^CG 1647 CGCG Tsp45I 517 GTGAC ^GT[GC]AC 2147 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI