##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI ApaI AscI Asp718I
AviII AvrII BbrPI BcgI BfrI
BglI BglII BlnI BsaI BsaWI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
ClaI CpoI DraIII EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII KpnI KspI MfeI MluI
MroI MunI MvnI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 21 GGCGCC G[GA]^CG[CT]C
AflIII 3866 ACACGT A^C[GA][CT]GT
AhdI 2558 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3261 AGCGCT AGC^GCT
AspEI 2558 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3372 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3112 CCCGAG C^[CT]CG[GA]G
BamHI 3617 GGATCC G^GATCC
BbeI 21 GGCGCC GGCGC^C
BcnI 1006 CCCGG CC^[GC]GG
BsaAI 538 TACGTG [CT]AC^GT[GA]
BsaHI 21 GGCGCC G[GA]^CG[CT]C
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsoBI 3112 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2062 TGTACA T^GTACA
BsrGI 2062 TGTACA T^GTACA
Bst1107I 1249 GTATAC GTA^TAC
BstZ17I 1249 GTATAC GTA^TAC
DraII 2187 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 26 CGGCCG C^GGCCG
Eam1105I 2558 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 26 CGGCCG C^GGCCG
Eco47III 3261 AGCGCT AGC^GCT
Eco52I 26 CGGCCG C^GGCCG
EcoNI 2430 CCTTAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2187 AGGTCCT [GA]G^G[ACGT]CC[CT]
FauI 1033 GCGGG CCCGC(4/6)
Hin1I 21 GGCGCC G[GA]^CG[CT]C
HpaI 2854 GTTAAC GTT^AAC
KasI 21 GGCGCC G^GCGCC
NaeI 24 GCCGGC GCC^GGC
NarI 21 GGCGCC GG^CGCC
NciI 1006 CCCGG CC^[GC]GG
NgoMIV 24 GCCGGC G^CCGGC
PflFI 3372 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 162 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2187 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3272 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 81 GAGCTC GAGCT^C
SbfI 1975 CCTGCAGG CCTGCA^GG
ScaI 747 AGTACT AGT^ACT
SfoI 21 GGCGCC GGC^GCC
Sse8387I 1975 CCTGCAGG CCTGCA^GG
SspBI 2062 TGTACA T^GTACA
Tth111I 3372 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 162 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1888 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1249 GTATAC GT^[AC][GT]AC
2346 GTCTAC
Alw44I 1948 GTGCAC G^TGCAC
3253 GTGCAC
AocI 2430 CCTTAGG CC^T[ACGT]AGG
3569 CCTGAGG
ApaLI 1948 GTGCAC G^TGCAC
3253 GTGCAC
AseI 810 ATTAAT AT^TAAT
1123 ATTAAT
AsnI 810 ATTAAT AT^TAAT
1123 ATTAAT
Asp700I 393 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2638 GAAAATTTTC
BanI 21 GGCGCC G^G[CT][GA]CC
3313 GGCACC
BbvCI 2907 CCTCAGC CCTCAGC(-5/-2)
1316 GCTGAGG
BciVI 3232 GGATAC GTATCC(6/5)
2173 GTATCC
BclI 434 TGATCA T^GATCA
503 TGATCA
BsaBI 131 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2354 GATGAGCATC
BspHI 605 TCATGA T^CATGA
3333 TCATGA
BsrFI 24 GCCGGC [GA]^CCGG[CT]
64 GCCGGT
BstAPI 526 GCAAATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1722 GCAGAGTGTGC
Bsu36I 2430 CCTTAGG CC^T[ACGT]AGG
3569 CCTGAGG
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
64 GCCGGT
DraI 3448 TTTAAA TTT^AAA
3741 TTTAAA
DrdI 1973 GACCTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3555 GACTCAGCTGTC
Eco81I 2430 CCTTAGG CC^T[ACGT]AGG
3569 CCTGAGG
FbaI 434 TGATCA T^GATCA
503 TGATCA
HincII 103 GTCAAC GT[CT]^[GA]AC
2854 GTTAAC
HindII 103 GTCAAC GT[CT]^[GA]AC
2854 GTTAAC
MamI 131 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2354 GATGAGCATC
NcoI 2764 CCATGG C^CATGG
3052 CCATGG
PshBI 810 ATTAAT AT^TAAT
1123 ATTAAT
RcaI 605 TCATGA T^CATGA
3333 TCATGA
SapI 928 GAAGAGC GCTCTTC(1/4)
2239 GAAGAGC
SmlI 388 CTCAAG C^T[CT][GA]AG
2895 CTCAAG
TaqI 230 TCGA T^CGA
246 TCGA
TfiI 556 GATTC G^A[AT]TC
609 GATTC
TthHB8I 230 TCGA T^CGA
246 TCGA
XmnI 393 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2638 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanII BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII