################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI ApaI AscI Asp718I AviII AvrII BbrPI BcgI BfrI BglI BglII BlnI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BssHII BssSI BstBI BstEII BstPI BstUI BstXI ClaI CpoI DraIII EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HindIII KpnI KspI MfeI MluI MroI MunI MvnI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 21 GGCGCC G[GA]^CG[CT]C AflIII 3866 ACACGT A^C[GA][CT]GT AhdI 2558 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3261 AGCGCT AGC^GCT AspEI 2558 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3372 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 3112 CCCGAG C^[CT]CG[GA]G BamHI 3617 GGATCC G^GATCC BbeI 21 GGCGCC GGCGC^C BcnI 1006 CCCGG CC^[GC]GG BsaAI 538 TACGTG [CT]AC^GT[GA] BsaHI 21 GGCGCC G[GA]^CG[CT]C BsiEI 26 CGGCCG CG[GA][CT]^CG BsoBI 3112 CCCGAG C^[CT]CG[GA]G Bsp1407I 2062 TGTACA T^GTACA BsrGI 2062 TGTACA T^GTACA Bst1107I 1249 GTATAC GTA^TAC BstZ17I 1249 GTATAC GTA^TAC DraII 2187 AGGTCCT [GA]G^G[ACGT]CC[CT] EagI 26 CGGCCG C^GGCCG Eam1105I 2558 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 26 CGGCCG C^GGCCG Eco47III 3261 AGCGCT AGC^GCT Eco52I 26 CGGCCG C^GGCCG EcoNI 2430 CCTTAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2187 AGGTCCT [GA]G^G[ACGT]CC[CT] FauI 1033 GCGGG CCCGC(4/6) Hin1I 21 GGCGCC G[GA]^CG[CT]C HpaI 2854 GTTAAC GTT^AAC KasI 21 GGCGCC G^GCGCC NaeI 24 GCCGGC GCC^GGC NarI 21 GGCGCC GG^CGCC NciI 1006 CCCGG CC^[GC]GG NgoMIV 24 GCCGGC G^CCGGC PflFI 3372 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 162 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2187 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 3272 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 81 GAGCTC GAGCT^C SbfI 1975 CCTGCAGG CCTGCA^GG ScaI 747 AGTACT AGT^ACT SfoI 21 GGCGCC GGC^GCC Sse8387I 1975 CCTGCAGG CCTGCA^GG SspBI 2062 TGTACA T^GTACA Tth111I 3372 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 162 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1888 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 1249 GTATAC GT^[AC][GT]AC 2346 GTCTAC Alw44I 1948 GTGCAC G^TGCAC 3253 GTGCAC AocI 2430 CCTTAGG CC^T[ACGT]AGG 3569 CCTGAGG ApaLI 1948 GTGCAC G^TGCAC 3253 GTGCAC AseI 810 ATTAAT AT^TAAT 1123 ATTAAT AsnI 810 ATTAAT AT^TAAT 1123 ATTAAT Asp700I 393 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2638 GAAAATTTTC BanI 21 GGCGCC G^G[CT][GA]CC 3313 GGCACC BbvCI 2907 CCTCAGC CCTCAGC(-5/-2) 1316 GCTGAGG BciVI 3232 GGATAC GTATCC(6/5) 2173 GTATCC BclI 434 TGATCA T^GATCA 503 TGATCA BsaBI 131 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2354 GATGAGCATC BspHI 605 TCATGA T^CATGA 3333 TCATGA BsrFI 24 GCCGGC [GA]^CCGG[CT] 64 GCCGGT BstAPI 526 GCAAATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1722 GCAGAGTGTGC Bsu36I 2430 CCTTAGG CC^T[ACGT]AGG 3569 CCTGAGG Cfr10I 24 GCCGGC [GA]^CCGG[CT] 64 GCCGGT DraI 3448 TTTAAA TTT^AAA 3741 TTTAAA DrdI 1973 GACCTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3555 GACTCAGCTGTC Eco81I 2430 CCTTAGG CC^T[ACGT]AGG 3569 CCTGAGG FbaI 434 TGATCA T^GATCA 503 TGATCA HincII 103 GTCAAC GT[CT]^[GA]AC 2854 GTTAAC HindII 103 GTCAAC GT[CT]^[GA]AC 2854 GTTAAC MamI 131 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2354 GATGAGCATC NcoI 2764 CCATGG C^CATGG 3052 CCATGG PshBI 810 ATTAAT AT^TAAT 1123 ATTAAT RcaI 605 TCATGA T^CATGA 3333 TCATGA SapI 928 GAAGAGC GCTCTTC(1/4) 2239 GAAGAGC SmlI 388 CTCAAG C^T[CT][GA]AG 2895 CTCAAG TaqI 230 TCGA T^CGA 246 TCGA TfiI 556 GATTC G^A[AT]TC 609 GATTC TthHB8I 230 TCGA T^CGA 246 TCGA XmnI 393 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2638 GAAAATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII