##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspI AviII AvrII BbeI
BbrPI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eco47III EcoT22I FseI
FspI HpaI KasI MamI MfeI
MluI MroI MunI NarI NdeI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1885 GGTACC G^GTACC
AclI 2684 AACGTT AA^CGTT
AcsI 2667 GAATTC [GA]^AATT[CT]
AflIII 664 ACATGT A^C[GA][CT]GT
AhdI 4412 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2667 GAATTC [GA]^AATT[CT]
Asp718I 1885 GGTACC G^GTACC
AspEI 4412 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1224 GGATCC G^GATCC
BclI 3856 TGATCA T^GATCA
BsaI 651 GAGACC GGTCTC(1/5)
BsaWI 63 ACCGGA [AT]^CCGG[AT]
BsmBI 2485 CGTCTC CGTCTC(1/5)
BspLU11I 664 ACATGT A^CATGT
BsrDI 3669 GCAATG GCAATG(2/0)
Eam1105I 4412 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2667 GAATTC G^AATTC
EcoRV 1651 GATATC GAT^ATC
FbaI 3856 TGATCA T^GATCA
HincII 3775 GTTGAC GT[CT]^[GA]AC
HindII 3775 GTTGAC GT[CT]^[GA]AC
HindIII 4196 AAGCTT A^AGCTT
KpnI 1885 GGTACC GGTAC^C
KspI 123 CCGCGG CCGC^GG
MseI 4129 TTAA T^TAA
NotI 474 GCGGCCGC GC^GGCCGC
PaeR7I 1674 CTCGAG C^TCGAG
PshAI 2390 GACGAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2684 AACGTT AA^CGTT
PvuII 3570 CAGCTG CAG^CTG
SacII 123 CCGCGG CCGC^GG
ScaI 688 AGTACT AGT^ACT
SexAI 2787 ACCTGGT A^CC[AT]GGT
SmaI 185 CCCGGG CCC^GGG
SspI 4125 AATATT AAT^ATT
Tru9I 4129 TTAA T^TAA
XhoI 1674 CTCGAG C^TCGAG
XmaCI 185 CCCGGG C^CCGGG
XmaI 185 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 20 GACGCC G[GA]^CG[CT]C
2483 GGCGTC
AocI 1620 CCTGAGG CC^T[ACGT]AGG
4110 CCTGAGG
ApaI 2202 GGGCCC GGGCC^C
4322 GGGCCC
AvaI 185 CCCGGG C^[CT]CG[GA]G
1674 CTCGAG
BalI 730 TGGCCA TGG^CCA
2751 TGGCCA
BsaHI 20 GACGCC G[GA]^CG[CT]C
2483 GGCGTC
BsiEI 126 CGGCCG CG[GA][CT]^CG
475 CGGCCG
BsoBI 185 CCCGGG C^[CT]CG[GA]G
1674 CTCGAG
Bsp1407I 1692 TGTACA T^GTACA
3450 TGTACA
BsrGI 1692 TGTACA T^GTACA
3450 TGTACA
BstAPI 233 GCACTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
520 GCATCACCTGC
BstEII 1768 GGTTACC G^GT[ACGT]ACC
1869 GGTCACC
BstPI 1768 GGTTACC G^GT[ACGT]ACC
1869 GGTCACC
Bsu36I 1620 CCTGAGG CC^T[ACGT]AGG
4110 CCTGAGG
EagI 126 CGGCCG C^GGCCG
475 CGGCCG
EclXI 126 CGGCCG C^GGCCG
475 CGGCCG
Eco52I 126 CGGCCG C^GGCCG
475 CGGCCG
Eco81I 1620 CCTGAGG CC^T[ACGT]AGG
4110 CCTGAGG
EcoO65I 1768 GGTTACC G^GT[ACGT]ACC
1869 GGTCACC
HaeII 462 AGCGCC [GA]GCGC^[CT]
4177 GGCGCT
Hin1I 20 GACGCC G[GA]^CG[CT]C
2483 GGCGTC
MluNI 730 TGGCCA TGG^CCA
2751 TGGCCA
MscI 730 TGGCCA TGG^CCA
2751 TGGCCA
NcoI 499 CCATGG C^CATGG
3175 CCATGG
NheI 401 GCTAGC G^CTAGC
4342 GCTAGC
SacI 414 GAGCTC GAGCT^C
816 GAGCTC
SspBI 1692 TGTACA T^GTACA
3450 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AspHI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII