################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AhdI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbrPI BglII BlnI BsaAI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FseI FspI Hin1I HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 1870 CGCG CG^CG AflII 84 CTTAAG C^TTAAG AgeI 1655 ACCGGT A^CCGGT Alw44I 708 GTGCAC G^TGCAC AocI 390 CCTGAGG CC^T[ACGT]AGG Aor51HI 37 AGCGCT AGC^GCT ApaI 2295 GGGCCC GGGCC^C ApaLI 708 GTGCAC G^TGCAC AspI 1632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2702 GGATCC G^GATCC BciVI 2321 GGATAC GTATCC(6/5) BclI 622 TGATCA T^GATCA BfrI 84 CTTAAG C^TTAAG BglI 1222 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 956 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspDI 1500 ATCGAT AT^CGAT BsrBI 134 GAGCGG CCGCTC(-3/-3) BsrFI 1655 ACCGGT [GA]^CCGG[CT] BstEII 3476 GGTGACC G^GT[ACGT]ACC BstPI 3476 GGTGACC G^GT[ACGT]ACC BstUI 1870 CGCG CG^CG BstXI 2326 CCACGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 390 CCTGAGG CC^T[ACGT]AGG Cfr10I 1655 ACCGGT [GA]^CCGG[CT] ClaI 1500 ATCGAT AT^CGAT Eco47III 37 AGCGCT AGC^GCT Eco81I 390 CCTGAGG CC^T[ACGT]AGG EcoO65I 3476 GGTGACC G^GT[ACGT]ACC EcoT22I 2933 ATGCAT ATGCA^T FbaI 622 TGATCA T^GATCA HgaI 703 GCGTC GACGC(5/10) HindIII 1308 AAGCTT A^AGCTT MamI 956 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1870 CGCG CG^CG NheI 1301 GCTAGC G^CTAGC NsiI 2933 ATGCAT ATGCA^T PflFI 1632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2479 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1655 ACCGGT A^CCGGT PshAI 663 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SmaI 27 CCCGGG CCC^GGG SpeI 3376 ACTAGT A^CTAGT Tth111I 1632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2479 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 27 CCCGGG C^CCGGG XmaI 27 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccI 1638 GTCTAC GT^[AC][GT]AC 1752 GTAGAC BalI 2173 TGGCCA TGG^CCA 3156 TGGCCA BsmI 2211 GAATGC GAATGC(1/-1) 296 GCATTC BsrDI 2850 CATTGC GCAATG(2/0) 2913 GCAATG BstAPI 17 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3279 GCAGCCAGTGC DraIII 18 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 3471 CACTTGGTG HincII 216 GTCAAC GT[CT]^[GA]AC 2403 GTCAAC HindII 216 GTCAAC GT[CT]^[GA]AC 2403 GTCAAC MluNI 2173 TGGCCA TGG^CCA 3156 TGGCCA MscI 2173 TGGCCA TGG^CCA 3156 TGGCCA NcoI 1879 CCATGG C^CATGG 1939 CCATGG PpuMI 88 AGGTCCT [GA]G^G[AT]CC[CT] 2167 GGGTCCT PstI 789 CTGCAG CTGCA^G 2469 CTGCAG PvuII 57 CAGCTG CAG^CTG 675 CAGCTG XcmI 830 CCAGGAGGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2847 CCACATTGCTTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII