##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbeI BbrPI BglII BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FseI FspI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspI NspV PacI PaeR7I PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 1870 CGCG CG^CG
AflII 84 CTTAAG C^TTAAG
AgeI 1655 ACCGGT A^CCGGT
Alw44I 708 GTGCAC G^TGCAC
AocI 390 CCTGAGG CC^T[ACGT]AGG
Aor51HI 37 AGCGCT AGC^GCT
ApaI 2295 GGGCCC GGGCC^C
ApaLI 708 GTGCAC G^TGCAC
AspI 1632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2702 GGATCC G^GATCC
BciVI 2321 GGATAC GTATCC(6/5)
BclI 622 TGATCA T^GATCA
BfrI 84 CTTAAG C^TTAAG
BglI 1222 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 956 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 1500 ATCGAT AT^CGAT
BsrBI 134 GAGCGG CCGCTC(-3/-3)
BsrFI 1655 ACCGGT [GA]^CCGG[CT]
BstEII 3476 GGTGACC G^GT[ACGT]ACC
BstPI 3476 GGTGACC G^GT[ACGT]ACC
BstUI 1870 CGCG CG^CG
BstXI 2326 CCACGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 390 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1655 ACCGGT [GA]^CCGG[CT]
ClaI 1500 ATCGAT AT^CGAT
Eco47III 37 AGCGCT AGC^GCT
Eco81I 390 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3476 GGTGACC G^GT[ACGT]ACC
EcoT22I 2933 ATGCAT ATGCA^T
FbaI 622 TGATCA T^GATCA
HgaI 703 GCGTC GACGC(5/10)
HindIII 1308 AAGCTT A^AGCTT
MamI 956 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1870 CGCG CG^CG
NheI 1301 GCTAGC G^CTAGC
NsiI 2933 ATGCAT ATGCA^T
PflFI 1632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2479 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1655 ACCGGT A^CCGGT
PshAI 663 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 27 CCCGGG CCC^GGG
SpeI 3376 ACTAGT A^CTAGT
Tth111I 1632 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2479 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 27 CCCGGG C^CCGGG
XmaI 27 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 1638 GTCTAC GT^[AC][GT]AC
1752 GTAGAC
BalI 2173 TGGCCA TGG^CCA
3156 TGGCCA
BsmI 2211 GAATGC GAATGC(1/-1)
296 GCATTC
BsrDI 2850 CATTGC GCAATG(2/0)
2913 GCAATG
BstAPI 17 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3279 GCAGCCAGTGC
DraIII 18 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
3471 CACTTGGTG
HincII 216 GTCAAC GT[CT]^[GA]AC
2403 GTCAAC
HindII 216 GTCAAC GT[CT]^[GA]AC
2403 GTCAAC
MluNI 2173 TGGCCA TGG^CCA
3156 TGGCCA
MscI 2173 TGGCCA TGG^CCA
3156 TGGCCA
NcoI 1879 CCATGG C^CATGG
1939 CCATGG
PpuMI 88 AGGTCCT [GA]G^G[AT]CC[CT]
2167 GGGTCCT
PstI 789 CTGCAG CTGCA^G
2469 CTGCAG
PvuII 57 CAGCTG CAG^CTG
675 CAGCTG
XcmI 830 CCAGGAGGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2847 CCACATTGCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII