##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AhdI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BglII       BlnI        BsaAI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspEI       BspHI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoRI       EcoRV       FseI        FspI        
Hin1I       HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NspI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII        1870  CGCG                 CG^CG
AflII          84  CTTAAG               C^TTAAG
AgeI         1655  ACCGGT               A^CCGGT
Alw44I        708  GTGCAC               G^TGCAC
AocI          390  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        37  AGCGCT               AGC^GCT
ApaI         2295  GGGCCC               GGGCC^C
ApaLI         708  GTGCAC               G^TGCAC
AspI         1632  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2702  GGATCC               G^GATCC
BciVI        2321  GGATAC               GTATCC(6/5)
BclI          622  TGATCA               T^GATCA
BfrI           84  CTTAAG               C^TTAAG
BglI         1222  GCCTGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         956  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI        1500  ATCGAT               AT^CGAT
BsrBI         134  GAGCGG               CCGCTC(-3/-3)
BsrFI        1655  ACCGGT               [GA]^CCGG[CT]
BstEII       3476  GGTGACC              G^GT[ACGT]ACC
BstPI        3476  GGTGACC              G^GT[ACGT]ACC
BstUI        1870  CGCG                 CG^CG
BstXI        2326  CCACGCACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        390  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1655  ACCGGT               [GA]^CCGG[CT]
ClaI         1500  ATCGAT               AT^CGAT
Eco47III       37  AGCGCT               AGC^GCT
Eco81I        390  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      3476  GGTGACC              G^GT[ACGT]ACC
EcoT22I      2933  ATGCAT               ATGCA^T
FbaI          622  TGATCA               T^GATCA
HgaI          703  GCGTC                GACGC(5/10)
HindIII      1308  AAGCTT               A^AGCTT
MamI          956  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1870  CGCG                 CG^CG
NheI         1301  GCTAGC               G^CTAGC
NsiI         2933  ATGCAT               ATGCA^T
PflFI        1632  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2479  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1655  ACCGGT               A^CCGGT
PshAI         663  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI           27  CCCGGG               CCC^GGG
SpeI         3376  ACTAGT               A^CTAGT
Tth111I      1632  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2479  CCAAGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          27  CCCGGG               C^CCGGG
XmaI           27  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI         1638  GTCTAC               GT^[AC][GT]AC
             1752  GTAGAC              
BalI         2173  TGGCCA               TGG^CCA
             3156  TGGCCA              
BsmI         2211  GAATGC               GAATGC(1/-1)
              296  GCATTC              
BsrDI        2850  CATTGC               GCAATG(2/0)
             2913  GCAATG              
BstAPI         17  GCACACCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3279  GCAGCCAGTGC         
DraIII         18  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3471  CACTTGGTG           
HincII        216  GTCAAC               GT[CT]^[GA]AC
             2403  GTCAAC              
HindII        216  GTCAAC               GT[CT]^[GA]AC
             2403  GTCAAC              
MluNI        2173  TGGCCA               TGG^CCA
             3156  TGGCCA              
MscI         2173  TGGCCA               TGG^CCA
             3156  TGGCCA              
NcoI         1879  CCATGG               C^CATGG
             1939  CCATGG              
PpuMI          88  AGGTCCT              [GA]G^G[AT]CC[CT]
             2167  GGGTCCT             
PstI          789  CTGCAG               CTGCA^G
             2469  CTGCAG              
PvuII          57  CAGCTG               CAG^CTG
              675  CAGCTG              
XcmI          830  CCAGGAGGTGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2847  CCACATTGCTTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvCI       BbvI        
BcgI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII