##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AspEI AspI AviII
BcgI BclI BfrI BglII BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BssSI BstBI BstEII BstPI
ClaI CpoI Eam1105I EcoO65I FbaI
FseI FspI HindIII KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NspV PacI PflFI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1041 GGTACC G^GTACC
AccI 4439 GTATAC GT^[AC][GT]AC
AccII 4334 CGCG CG^CG
AflIII 3155 ACATGT A^C[GA][CT]GT
Alw44I 4089 GTGCAC G^TGCAC
ApaLI 4089 GTGCAC G^TGCAC
AseI 2710 ATTAAT AT^TAAT
AsnI 2710 ATTAAT AT^TAAT
Asp700I 557 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1041 GGTACC G^GTACC
BbeI 210 GGCGCC GGCGC^C
BsiEI 102 CGGCCG CG[GA][CT]^CG
BsmBI 1404 CGTCTC CGTCTC(1/5)
BsmI 1951 GAATGC GAATGC(1/-1)
Bsp1407I 4402 TGTACA T^GTACA
BspLU11I 3155 ACATGT A^CATGT
BsrGI 4402 TGTACA T^GTACA
BssHII 4333 GCGCGC G^CGCGC
Bst1107I 4439 GTATAC GTA^TAC
BstUI 4334 CGCG CG^CG
BstZ17I 4439 GTATAC GTA^TAC
EagI 102 CGGCCG C^GGCCG
EclXI 102 CGGCCG C^GGCCG
Eco52I 102 CGGCCG C^GGCCG
EcoNI 3737 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3661 GATATC GAT^ATC
EcoT22I 4305 ATGCAT ATGCA^T
HpaI 3209 GTTAAC GTT^AAC
KasI 210 GGCGCC G^GCGCC
KpnI 1041 GGTACC GGTAC^C
MvnI 4334 CGCG CG^CG
NaeI 4389 GCCGGC GCC^GGC
NarI 210 GGCGCC GG^CGCC
NcoI 376 CCATGG C^CATGG
NgoMIV 4389 GCCGGC G^CCGGC
NsiI 4305 ATGCAT ATGCA^T
PaeR7I 2311 CTCGAG C^TCGAG
PshAI 1164 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2710 ATTAAT AT^TAAT
PstI 1181 CTGCAG CTGCA^G
SapI 3728 GCTCTTC GCTCTTC(1/4)
ScaI 1192 AGTACT AGT^ACT
SexAI 2903 ACCTGGT A^CC[AT]GGT
SfiI 176 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 210 GGCGCC GGC^GCC
SspBI 4402 TGTACA T^GTACA
XbaI 4459 TCTAGA T^CTAGA
XhoI 2311 CTCGAG C^TCGAG
XmnI 557 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 19 GACGCC G[GA]^CG[CT]C
210 GGCGCC
Aor51HI 526 AGCGCT AGC^GCT
4411 AGCGCT
ApaI 184 GGGCCC GGGCC^C
2669 GGGCCC
BalI 2909 TGGCCA TGG^CCA
4082 TGGCCA
BamHI 2331 GGATCC G^GATCC
3271 GGATCC
BbrPI 1413 CACGTG CAC^GTG
4086 CACGTG
BbvCI 2879 CCTCAGC CCTCAGC(-5/-2)
2392 GCTGAGG
BciVI 4045 GTATCC GTATCC(6/5)
4056 GTATCC
BlpI 129 GCTCAGC GC^T[ACGT]AGC
932 GCTGAGC
Bpu1102I 129 GCTCAGC GC^T[ACGT]AGC
932 GCTGAGC
BsaHI 19 GACGCC G[GA]^CG[CT]C
210 GGCGCC
BstXI 376 CCATGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2368 CCACTAAGATGG
CelII 129 GCTCAGC GC^T[ACGT]AGC
932 GCTGAGC
DrdI 420 GACCGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1884 GACTGCTGTGTC
Eco47III 526 AGCGCT AGC^GCT
4411 AGCGCT
EcoRI 561 GAATTC G^AATTC
4240 GAATTC
HgaI 19 GACGC GACGC(5/10)
2691 GCGTC
Hin1I 19 GACGCC G[GA]^CG[CT]C
210 GGCGCC
HincII 3011 GTTGAC GT[CT]^[GA]AC
3209 GTTAAC
HindII 3011 GTTGAC GT[CT]^[GA]AC
3209 GTTAAC
MluNI 2909 TGGCCA TGG^CCA
4082 TGGCCA
MscI 2909 TGGCCA TGG^CCA
4082 TGGCCA
PmaCI 1413 CACGTG CAC^GTG
4086 CACGTG
PmlI 1413 CACGTG CAC^GTG
4086 CACGTG
SphI 940 GCATGC GCATG^C
1144 GCATGC
XcmI 2317 CCAGCCTCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3044 CCAGTCCTTTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII AvrII BanI BanII BbsI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI