################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AscI AspEI AspI AviII BcgI BclI BfrI BglII BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BssSI BstBI BstEII BstPI ClaI CpoI Eam1105I EcoO65I FbaI FseI FspI HindIII KspI MamI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PflFI PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1041 GGTACC G^GTACC AccI 4439 GTATAC GT^[AC][GT]AC AccII 4334 CGCG CG^CG AflIII 3155 ACATGT A^C[GA][CT]GT Alw44I 4089 GTGCAC G^TGCAC ApaLI 4089 GTGCAC G^TGCAC AseI 2710 ATTAAT AT^TAAT AsnI 2710 ATTAAT AT^TAAT Asp700I 557 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1041 GGTACC G^GTACC BbeI 210 GGCGCC GGCGC^C BsiEI 102 CGGCCG CG[GA][CT]^CG BsmBI 1404 CGTCTC CGTCTC(1/5) BsmI 1951 GAATGC GAATGC(1/-1) Bsp1407I 4402 TGTACA T^GTACA BspLU11I 3155 ACATGT A^CATGT BsrGI 4402 TGTACA T^GTACA BssHII 4333 GCGCGC G^CGCGC Bst1107I 4439 GTATAC GTA^TAC BstUI 4334 CGCG CG^CG BstZ17I 4439 GTATAC GTA^TAC EagI 102 CGGCCG C^GGCCG EclXI 102 CGGCCG C^GGCCG Eco52I 102 CGGCCG C^GGCCG EcoNI 3737 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3661 GATATC GAT^ATC EcoT22I 4305 ATGCAT ATGCA^T HpaI 3209 GTTAAC GTT^AAC KasI 210 GGCGCC G^GCGCC KpnI 1041 GGTACC GGTAC^C MvnI 4334 CGCG CG^CG NaeI 4389 GCCGGC GCC^GGC NarI 210 GGCGCC GG^CGCC NcoI 376 CCATGG C^CATGG NgoMIV 4389 GCCGGC G^CCGGC NsiI 4305 ATGCAT ATGCA^T PaeR7I 2311 CTCGAG C^TCGAG PshAI 1164 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2710 ATTAAT AT^TAAT PstI 1181 CTGCAG CTGCA^G SapI 3728 GCTCTTC GCTCTTC(1/4) ScaI 1192 AGTACT AGT^ACT SexAI 2903 ACCTGGT A^CC[AT]GGT SfiI 176 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 210 GGCGCC GGC^GCC SspBI 4402 TGTACA T^GTACA XbaI 4459 TCTAGA T^CTAGA XhoI 2311 CTCGAG C^TCGAG XmnI 557 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 19 GACGCC G[GA]^CG[CT]C 210 GGCGCC Aor51HI 526 AGCGCT AGC^GCT 4411 AGCGCT ApaI 184 GGGCCC GGGCC^C 2669 GGGCCC BalI 2909 TGGCCA TGG^CCA 4082 TGGCCA BamHI 2331 GGATCC G^GATCC 3271 GGATCC BbrPI 1413 CACGTG CAC^GTG 4086 CACGTG BbvCI 2879 CCTCAGC CCTCAGC(-5/-2) 2392 GCTGAGG BciVI 4045 GTATCC GTATCC(6/5) 4056 GTATCC BlpI 129 GCTCAGC GC^T[ACGT]AGC 932 GCTGAGC Bpu1102I 129 GCTCAGC GC^T[ACGT]AGC 932 GCTGAGC BsaHI 19 GACGCC G[GA]^CG[CT]C 210 GGCGCC BstXI 376 CCATGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2368 CCACTAAGATGG CelII 129 GCTCAGC GC^T[ACGT]AGC 932 GCTGAGC DrdI 420 GACCGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1884 GACTGCTGTGTC Eco47III 526 AGCGCT AGC^GCT 4411 AGCGCT EcoRI 561 GAATTC G^AATTC 4240 GAATTC HgaI 19 GACGC GACGC(5/10) 2691 GCGTC Hin1I 19 GACGCC G[GA]^CG[CT]C 210 GGCGCC HincII 3011 GTTGAC GT[CT]^[GA]AC 3209 GTTAAC HindII 3011 GTTGAC GT[CT]^[GA]AC 3209 GTTAAC MluNI 2909 TGGCCA TGG^CCA 4082 TGGCCA MscI 2909 TGGCCA TGG^CCA 4082 TGGCCA PmaCI 1413 CACGTG CAC^GTG 4086 CACGTG PmlI 1413 CACGTG CAC^GTG 4086 CACGTG SphI 940 GCATGC GCATG^C 1144 GCATGC XcmI 2317 CCAGCCTCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3044 CCAGTCCTTTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII AvrII BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI