##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AclI AgeI Aor51HI AscI AseI
AsnI Asp700I AspI AvrII BamHI
BbrPI BbvCI BclI BlnI BsaAI
BsiEI BsiWI BspLU11I BssHII Bst1107I
BstZ17I CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRV FbaI
FseI HgaI KspI MluI NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 3235 GTAGAC GT^[AC][GT]AC
AccII 56 CGCG CG^CG
AccIII 3019 TCCGGA T^CCGGA
AflII 3805 CTTAAG C^TTAAG
AflIII 591 ACACGT A^C[GA][CT]GT
AhdI 3126 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2954 GTGCAC G^TGCAC
ApaLI 2954 GTGCAC G^TGCAC
AspEI 3126 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 271 TGCGCA TGC^GCA
BbeI 14 GGCGCC GGCGC^C
BciVI 466 GGATAC GTATCC(6/5)
BfrI 3805 CTTAAG C^TTAAG
BlpI 4152 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4152 GCTGAGC GC^T[ACGT]AGC
BsaBI 293 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3019 TCCGGA [AT]^CCGG[AT]
BseAI 3019 TCCGGA T^CCGGA
BsmI 864 GCATTC GAATGC(1/-1)
Bsp1407I 2735 TGTACA T^GTACA
BspDI 413 ATCGAT AT^CGAT
BspEI 3019 TCCGGA T^CCGGA
BspHI 1561 TCATGA T^CATGA
BsrBI 3633 CCGCTC CCGCTC(-3/-3)
BsrGI 2735 TGTACA T^GTACA
BssSI 1148 CACGAG CACGAG(-5/-1)
BstBI 552 TTCGAA TT^CGAA
BstUI 56 CGCG CG^CG
CelII 4152 GCTGAGC GC^T[ACGT]AGC
ClaI 413 ATCGAT AT^CGAT
Eam1105I 3126 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3484 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3596 GAATTC G^AATTC
EcoT22I 1554 ATGCAT ATGCA^T
FspI 271 TGCGCA TGC^GCA
HpaI 2677 GTTAAC GTT^AAC
KasI 14 GGCGCC G^GCGCC
MamI 293 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4308 CAATTG C^AATTG
MroI 3019 TCCGGA T^CCGGA
MunI 4308 CAATTG C^AATTG
MvnI 56 CGCG CG^CG
NaeI 17 GCCGGC GCC^GGC
NarI 14 GGCGCC GG^CGCC
NdeI 3693 CATATG CA^TATG
NgoMIV 17 GCCGGC G^CCGGC
NsiI 1554 ATGCAT ATGCA^T
NspV 552 TTCGAA TT^CGAA
PaeR7I 4 CTCGAG C^TCGAG
RcaI 1561 TCATGA T^CATGA
SapI 3336 GAAGAGC GCTCTTC(1/4)
SexAI 1815 ACCTGGT A^CC[AT]GGT
SfoI 14 GGCGCC GGC^GCC
SfuI 552 TTCGAA TT^CGAA
SspBI 2735 TGTACA T^GTACA
XbaI 1887 TCTAGA T^CTAGA
XhoI 4 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AatII 1635 GACGTC GACGT^C
3742 GACGTC
BcgI 262 CGACGTGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3569 GCAAATTTATCG
BglII 2132 AGATCT A^GATCT
2929 AGATCT
BsgI 3047 CTGCAC GTGCAG(16/14)
4320 CTGCAC
BsrDI 429 CATTGC GCAATG(2/0)
821 GCAATG
BsrFI 17 GCCGGC [GA]^CCGG[CT]
745 ACCGGC
BstXI 1502 CCAGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2256 CCAACAAGATGG
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
745 ACCGGC
HindIII 3891 AAGCTT A^AGCTT
4354 AAGCTT
NcoI 2154 CCATGG C^CATGG
3353 CCATGG
NspI 1552 ACATGC [GA]CATG^[CT]
3378 GCATGT
PshAI 2100 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3756 GACTGGGGTC
PstI 22 CTGCAG CTGCA^G
2608 CTGCAG
StuI 1794 AGGCCT AGG^CCT
1941 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI