##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI BbeI BcgI
BfrI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DraI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI Hin1I
HindIII KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2599 GTCTAC GT^[AC][GT]AC
AcsI 2983 AAATTC [GA]^AATT[CT]
AflIII 2832 ACACGT A^C[GA][CT]GT
Alw44I 2629 GTGCAC G^TGCAC
AocI 114 CCTGAGG CC^T[ACGT]AGG
ApaLI 2629 GTGCAC G^TGCAC
ApoI 2983 AAATTC [GA]^AATT[CT]
AviII 2846 TGCGCA TGC^GCA
BamHI 2693 GGATCC G^GATCC
BbrPI 29 CACGTG CAC^GTG
BciVI 1906 GTATCC GTATCC(6/5)
BclI 3745 TGATCA T^GATCA
BsaAI 29 CACGTG [CT]AC^GT[GA]
BsiEI 3523 CGACCG CG[GA][CT]^CG
BspDI 139 ATCGAT AT^CGAT
BstAPI 3942 GCACGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2328 CCATAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 114 CCTGAGG CC^T[ACGT]AGG
ClaI 139 ATCGAT AT^CGAT
DraIII 2657 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2857 GACTGGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 114 CCTGAGG CC^T[ACGT]AGG
FbaI 3745 TGATCA T^GATCA
FspI 2846 TGCGCA TGC^GCA
HaeII 2006 AGCGCC [GA]GCGC^[CT]
HincII 911 GTTAAC GT[CT]^[GA]AC
HindII 911 GTTAAC GT[CT]^[GA]AC
HpaI 911 GTTAAC GTT^AAC
NheI 3481 GCTAGC G^CTAGC
NspI 365 ACATGC [GA]CATG^[CT]
PmaCI 29 CACGTG CAC^GTG
PmlI 29 CACGTG CAC^GTG
SacI 3474 GAGCTC GAGCT^C
SapI 2679 GAAGAGC GCTCTTC(1/4)
ScaI 83 AGTACT AGT^ACT
SfiI 711 GGCCACGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XbaI 1827 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Asp700I 2679 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2979 GAAGAAATTC
AvrII 786 CCTAGG C^CTAGG
3578 CCTAGG
BbvCI 422 CCTCAGC CCTCAGC(-5/-2)
3583 GCTGAGG
BglII 1488 AGATCT A^GATCT
2316 AGATCT
BlnI 786 CCTAGG C^CTAGG
3578 CCTAGG
BsmI 1725 GAATGC GAATGC(1/-1)
2772 GAATGC
Bsp1407I 2426 TGTACA T^GTACA
3980 TGTACA
BsrDI 1815 GCAATG GCAATG(2/0)
2454 GCAATG
BsrGI 2426 TGTACA T^GTACA
3980 TGTACA
BssSI 3498 CACGAG CACGAG(-5/-1)
2660 CTCGTG
HgaI 3534 GACGC GACGC(5/10)
3351 GCGTC
MaeII 30 ACGT A^CGT
2834 ACGT
PpuMI 17 AGGTCCT [GA]G^G[AT]CC[CT]
3043 AGGACCC
SspBI 2426 TGTACA T^GTACA
3980 TGTACA
XmnI 2679 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2979 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AfaI AhdI AluI
AlwI AlwNI ApaI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI