##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AvaI AviII
BamHI BbeI BbrPI BcgI BciVI
BfrI BsaHI BsaI BsiEI BsiWI
BsmBI BsoBI BspHI BsrBI BssHII
BstBI BstUI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I FseI
FspI HgaI Hin1I KasI KspI
MluI MvnI NaeI NarI NcoI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 4738 TCCGGA T^CCGGA
Alw44I 2603 GTGCAC G^TGCAC
ApaLI 2603 GTGCAC G^TGCAC
AspI 1703 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4744 CCTAGG C^CTAGG
BbvCI 1042 CCTCAGC CCTCAGC(-5/-2)
BglI 3312 GCCTACTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4744 CCTAGG C^CTAGG
BlpI 815 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 815 GCTGAGC GC^T[ACGT]AGC
BseAI 4738 TCCGGA T^CCGGA
BsmI 3027 GAATGC GAATGC(1/-1)
BspDI 2950 ATCGAT AT^CGAT
BspEI 4738 TCCGGA T^CCGGA
Bst1107I 955 GTATAC GTA^TAC
BstAPI 284 GCAACAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 955 GTATAC GTA^TAC
CelII 815 GCTGAGC GC^T[ACGT]AGC
CfoI 136 GCGC GCG^C
ClaI 2950 ATCGAT AT^CGAT
DrdI 4728 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1100 GAATTC G^AATTC
EcoRV 707 GATATC GAT^ATC
HaeII 135 AGCGCC [GA]GCGC^[CT]
HhaI 136 GCGC GCG^C
HinP1I 136 GCGC G^CGC
HpaI 1534 GTTAAC GTT^AAC
MroI 4738 TCCGGA T^CCGGA
NheI 1318 GCTAGC G^CTAGC
PflFI 1703 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3711 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2787 GCTCTTC GCTCTTC(1/4)
SfiI 3311 GGCCTACTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 3518 CTTGAG C^T[CT][GA]AG
Tth111I 1703 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 459 CCACGGGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 455 GGTACC G^GTACC
836 GGTACC
Asp718I 455 GGTACC G^GTACC
836 GGTACC
BglII 2262 AGATCT A^GATCT
4269 AGATCT
BsaBI 2917 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3908 GATTTTGATC
BsaWI 227 ACCGGA [AT]^CCGG[AT]
4738 TCCGGA
BsgI 927 CTGCAC GTGCAG(16/14)
4603 CTGCAC
BsrFI 91 GCCGGT [GA]^CCGG[CT]
2636 ACCGGC
BstEII 1520 GGTCACC G^GT[ACGT]ACC
2182 GGTCACC
BstPI 1520 GGTCACC G^GT[ACGT]ACC
2182 GGTCACC
BstXI 1073 CCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4786 CCAGCATTTTGG
Cfr10I 91 GCCGGT [GA]^CCGG[CT]
2636 ACCGGC
DsaI 459 CCACGG C^C[GA][CT]GG
2676 CCGTGG
EcoO65I 1520 GGTCACC G^GT[ACGT]ACC
2182 GGTCACC
EcoT22I 767 ATGCAT ATGCA^T
4842 ATGCAT
HincII 71 GTCAAC GT[CT]^[GA]AC
1534 GTTAAC
HindII 71 GTCAAC GT[CT]^[GA]AC
1534 GTTAAC
KpnI 455 GGTACC GGTAC^C
836 GGTACC
MamI 2917 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3908 GATTTTGATC
MfeI 288 CAATTG C^AATTG
4141 CAATTG
MunI 288 CAATTG C^AATTG
4141 CAATTG
NsiI 767 ATGCAT ATGCA^T
4842 ATGCAT
SacI 2429 GAGCTC GAGCT^C
3354 GAGCTC
ScaI 276 AGTACT AGT^ACT
2226 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmnI