################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AvaI AviII BamHI BbeI BbrPI BcgI BciVI BfrI BsaHI BsaI BsiEI BsiWI BsmBI BsoBI BspHI BsrBI BssHII BstBI BstUI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HgaI Hin1I KasI KspI MluI MvnI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 4738 TCCGGA T^CCGGA Alw44I 2603 GTGCAC G^TGCAC ApaLI 2603 GTGCAC G^TGCAC AspI 1703 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4744 CCTAGG C^CTAGG BbvCI 1042 CCTCAGC CCTCAGC(-5/-2) BglI 3312 GCCTACTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 4744 CCTAGG C^CTAGG BlpI 815 GCTGAGC GC^T[ACGT]AGC Bpu1102I 815 GCTGAGC GC^T[ACGT]AGC BseAI 4738 TCCGGA T^CCGGA BsmI 3027 GAATGC GAATGC(1/-1) BspDI 2950 ATCGAT AT^CGAT BspEI 4738 TCCGGA T^CCGGA Bst1107I 955 GTATAC GTA^TAC BstAPI 284 GCAACAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 955 GTATAC GTA^TAC CelII 815 GCTGAGC GC^T[ACGT]AGC CfoI 136 GCGC GCG^C ClaI 2950 ATCGAT AT^CGAT DrdI 4728 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1100 GAATTC G^AATTC EcoRV 707 GATATC GAT^ATC HaeII 135 AGCGCC [GA]GCGC^[CT] HhaI 136 GCGC GCG^C HinP1I 136 GCGC G^CGC HpaI 1534 GTTAAC GTT^AAC MroI 4738 TCCGGA T^CCGGA NheI 1318 GCTAGC G^CTAGC PflFI 1703 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3711 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2787 GCTCTTC GCTCTTC(1/4) SfiI 3311 GGCCTACTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 3518 CTTGAG C^T[CT][GA]AG Tth111I 1703 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 459 CCACGGGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Acc65I 455 GGTACC G^GTACC 836 GGTACC Asp718I 455 GGTACC G^GTACC 836 GGTACC BglII 2262 AGATCT A^GATCT 4269 AGATCT BsaBI 2917 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3908 GATTTTGATC BsaWI 227 ACCGGA [AT]^CCGG[AT] 4738 TCCGGA BsgI 927 CTGCAC GTGCAG(16/14) 4603 CTGCAC BsrFI 91 GCCGGT [GA]^CCGG[CT] 2636 ACCGGC BstEII 1520 GGTCACC G^GT[ACGT]ACC 2182 GGTCACC BstPI 1520 GGTCACC G^GT[ACGT]ACC 2182 GGTCACC BstXI 1073 CCAGAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4786 CCAGCATTTTGG Cfr10I 91 GCCGGT [GA]^CCGG[CT] 2636 ACCGGC DsaI 459 CCACGG C^C[GA][CT]GG 2676 CCGTGG EcoO65I 1520 GGTCACC G^GT[ACGT]ACC 2182 GGTCACC EcoT22I 767 ATGCAT ATGCA^T 4842 ATGCAT HincII 71 GTCAAC GT[CT]^[GA]AC 1534 GTTAAC HindII 71 GTCAAC GT[CT]^[GA]AC 1534 GTTAAC KpnI 455 GGTACC GGTAC^C 836 GGTACC MamI 2917 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3908 GATTTTGATC MfeI 288 CAATTG C^AATTG 4141 CAATTG MunI 288 CAATTG C^AATTG 4141 CAATTG NsiI 767 ATGCAT ATGCA^T 4842 ATGCAT SacI 2429 GAGCTC GAGCT^C 3354 GAGCTC ScaI 276 AGTACT AGT^ACT 2226 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BspMI BsrDI BsrGI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI