##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BcgI BclI BglI BlnI BsiEI
BsiWI BsmI BspDI BspHI BsrBI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FbaI FseI FspI KpnI
KspI MluI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 286 GACGTC GACGT^C
AccIII 1997 TCCGGA T^CCGGA
Alw44I 2156 GTGCAC G^TGCAC
ApaLI 2156 GTGCAC G^TGCAC
AvaI 527 CTCGGG C^[CT]CG[GA]G
BamHI 687 GGATCC G^GATCC
BanI 1951 GGCGCC G^G[CT][GA]CC
BbeI 1951 GGCGCC GGCGC^C
BbrPI 2738 CACGTG CAC^GTG
BbvCI 1861 GCTGAGG CCTCAGC(-5/-2)
BciVI 749 GGATAC GTATCC(6/5)
BlpI 637 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 637 GCTAAGC GC^T[ACGT]AGC
BsaAI 2738 CACGTG [CT]AC^GT[GA]
BseAI 1997 TCCGGA T^CCGGA
BsoBI 527 CTCGGG C^[CT]CG[GA]G
BspEI 1997 TCCGGA T^CCGGA
BsrFI 1511 ACCGGC [GA]^CCGG[CT]
BstBI 1369 TTCGAA TT^CGAA
BstXI 3962 CCAGTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 637 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1511 ACCGGC [GA]^CCGG[CT]
DraI 4544 TTTAAA TTT^AAA
DsaI 707 CCGTGG C^C[GA][CT]GG
EcoRV 295 GATATC GAT^ATC
EcoT14I 3575 CCAAGG C^C[AT][AT]GG
FauI 1896 GCGGG CCCGC(4/6)
HindIII 2570 AAGCTT A^AGCTT
HpaI 2815 GTTAAC GTT^AAC
KasI 1951 GGCGCC G^GCGCC
MfeI 2048 CAATTG C^AATTG
MroI 1997 TCCGGA T^CCGGA
MunI 2048 CAATTG C^AATTG
NarI 1951 GGCGCC GG^CGCC
NspV 1369 TTCGAA TT^CGAA
PacI 2545 TTAATTAA TTAAT^TAA
PmaCI 2738 CACGTG CAC^GTG
PmlI 2738 CACGTG CAC^GTG
PshAI 1095 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 3490 ACCAGGT A^CC[AT]GGT
SfoI 1951 GGCGCC GGC^GCC
SfuI 1369 TTCGAA TT^CGAA
SpeI 3286 ACTAGT A^CTAGT
SspI 2786 AATATT AAT^ATT
StyI 3575 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 482 GGGCCC GGGCC^C
2226 GGGCCC
BalI 2757 TGGCCA TGG^CCA
3327 TGGCCA
BsaBI 1545 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2476 GATGGGCATC
BsaI 457 GAGACC GGTCTC(1/5)
702 GGTCTC
BsaWI 500 ACCGGA [AT]^CCGG[AT]
1997 TCCGGA
Bsp1407I 3015 TGTACA T^GTACA
3356 TGTACA
BspMI 1892 ACCTGC ACCTGC(4/8)
4286 GCAGGT
BsrGI 3015 TGTACA T^GTACA
3356 TGTACA
EarI 1495 GAAGAG CTCTTC(1/4)
1936 GAAGAG
EcoRI 1449 GAATTC G^AATTC
4522 GAATTC
HaeII 137 AGCGCC [GA]GCGC^[CT]
1951 GGCGCC
Ksp632I 1495 GAAGAG CTCTTC(1/4)
1936 GAAGAG
MamI 1545 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2476 GATGGGCATC
MluNI 2757 TGGCCA TGG^CCA
3327 TGGCCA
MscI 2757 TGGCCA TGG^CCA
3327 TGGCCA
SacI 3773 GAGCTC GAGCT^C
4050 GAGCTC
SapI 1495 GAAGAGC GCTCTTC(1/4)
1936 GAAGAGC
ScaI 3680 AGTACT AGT^ACT
4333 AGTACT
SspBI 3015 TGTACA T^GTACA
3356 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII EaeI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI