##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbvCI
BcgI BclI BlnI BsaI BseAI
BsiWI BspDI BspEI BspHI BsrDI
BssSI BstAPI BstBI Bsu36I ClaI
CpoI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV FbaI FseI
FspI HpaI KpnI MfeI MluI
MroI MunI NotI NruI NspV
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SnaBI Sse8387I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2086 GACGTC GACGT^C
BalI 326 TGGCCA TGG^CCA
BbrPI 3282 CACGTG CAC^GTG
BglII 3794 AGATCT A^GATCT
BlpI 965 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 965 GCTTAGC GC^T[ACGT]AGC
BsaWI 2229 ACCGGA [AT]^CCGG[AT]
BspLU11I 3873 ACATGT A^CATGT
BsrBI 313 GAGCGG CCGCTC(-3/-3)
BstXI 1123 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 965 GCTTAGC GC^T[ACGT]AGC
DrdI 1926 GACCATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 161 CGGCCG C^GGCCG
EclXI 161 CGGCCG C^GGCCG
Eco52I 161 CGGCCG C^GGCCG
MluNI 326 TGGCCA TGG^CCA
MscI 326 TGGCCA TGG^CCA
NaeI 370 GCCGGC GCC^GGC
NdeI 2150 CATATG CA^TATG
NgoMIV 370 GCCGGC G^CCGGC
PacI 2365 TTAATTAA TTAAT^TAA
PflMI 744 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3282 CACGTG CAC^GTG
PmlI 3282 CACGTG CAC^GTG
SapI 3020 GCTCTTC GCTCTTC(1/4)
SgrAI 369 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 2717 ACTAGT A^CTAGT
SphI 1084 GCATGC GCATG^C
StuI 1716 AGGCCT AGG^CCT
Van91I 744 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1069 CCAGTGGATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 2785 CTTAAG C^TTAAG
3086 CTTAAG
BfrI 2785 CTTAAG C^TTAAG
3086 CTTAAG
BglI 275 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1276 GCCTCTGAGGC
BsaAI 2139 TACGTG [CT]AC^GT[GA]
3282 CACGTG
BsaBI 1135 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3711 GATAATTATC
BsgI 507 CTGCAC GTGCAG(16/14)
3997 CTGCAC
BsiEI 141 CGACCG CG[GA][CT]^CG
161 CGGCCG
BsmI 2416 GAATGC GAATGC(1/-1)
2754 GAATGC
Bsp1407I 2206 TGTACA T^GTACA
3232 TGTACA
BspMI 505 ACCTGC ACCTGC(4/8)
3783 ACCTGC
BsrFI 143 ACCGGC [GA]^CCGG[CT]
370 GCCGGC
BsrGI 2206 TGTACA T^GTACA
3232 TGTACA
BssHII 341 GCGCGC G^CGCGC
356 GCGCGC
BstEII 215 GGTGACC G^GT[ACGT]ACC
1494 GGTAACC
BstPI 215 GGTGACC G^GT[ACGT]ACC
1494 GGTAACC
Cfr10I 143 ACCGGC [GA]^CCGG[CT]
370 GCCGGC
EcoO65I 215 GGTGACC G^GT[ACGT]ACC
1494 GGTAACC
EcoT22I 2103 ATGCAT ATGCA^T
2147 ATGCAT
HgaI 1864 GACGC GACGC(5/10)
1944 GACGC
HindIII 634 AAGCTT A^AGCTT
3701 AAGCTT
MamI 1135 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3711 GATAATTATC
NheI 922 GCTAGC G^CTAGC
3276 GCTAGC
NsiI 2103 ATGCAT ATGCA^T
2147 ATGCAT
PleI 1576 GAGTC GAGTC(4/5)
3358 GAGTC
PpuMI 893 GGGACCT [GA]G^G[AT]CC[CT]
2002 GGGTCCC
PstI 3336 CTGCAG CTGCA^G
4083 CTGCAG
PvuII 941 CAGCTG CAG^CTG
2319 CAGCTG
SexAI 1850 ACCTGGT A^CC[AT]GGT
3181 ACCTGGT
SmaI 409 CCCGGG CCC^GGG
436 CCCGGG
SspBI 2206 TGTACA T^GTACA
3232 TGTACA
SwaI 3046 ATTTAAAT ATTT^AAAT
3058 ATTTAAAT
TaqI 496 TCGA T^CGA
3463 TCGA
TthHB8I 496 TCGA T^CGA
3463 TCGA
XbaI 849 TCTAGA T^CTAGA
2274 TCTAGA
XmaCI 409 CCCGGG C^CCGGG
436 CCCGGG
XmaI 409 CCCGGG C^CCGGG
436 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrI BssKI Bst1107I
BstF5I BstNI BstUI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI