################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AgeI AhdI AscI AspEI AviII AvrII BamHI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsiWI Bsp1407I BspDI BspHI BsrGI BssSI Bst1107I BstZ17I CelII ClaI CpoI Eam1105I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI MluI NdeI NheI NruI NsiI PacI PinAI PmeI RcaI RsrII SalI SbfI SexAI SfiI SgrAI SpeI Sse8387I SspBI SspI StuI SwaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1368 GTAGAC GT^[AC][GT]AC AccIII 220 TCCGGA T^CCGGA AclI 3550 AACGTT AA^CGTT Alw44I 911 GTGCAC G^TGCAC AocI 3831 CCTAAGG CC^T[ACGT]AGG ApaI 4254 GGGCCC GGGCC^C ApaLI 911 GTGCAC G^TGCAC AspI 3371 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 197 GGCGCC GGCGC^C BbrPI 3480 CACGTG CAC^GTG BciVI 3569 GGATAC GTATCC(6/5) BclI 4074 TGATCA T^GATCA BglII 4132 AGATCT A^GATCT BsaBI 1910 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 220 TCCGGA T^CCGGA BspEI 220 TCCGGA T^CCGGA BspLU11I 3573 ACATGT A^CATGT BstBI 642 TTCGAA TT^CGAA Bsu36I 3831 CCTAAGG CC^T[ACGT]AGG DrdI 1269 GACGGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3831 CCTAAGG CC^T[ACGT]AGG FbaI 4074 TGATCA T^GATCA KasI 197 GGCGCC G^GCGCC KspI 126 CCGCGG CCGC^GG MamI 1910 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 220 TCCGGA T^CCGGA NarI 197 GGCGCC GG^CGCC NspV 642 TTCGAA TT^CGAA PaeR7I 2771 CTCGAG C^TCGAG PflFI 3371 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3480 CACGTG CAC^GTG PmlI 3480 CACGTG CAC^GTG PpuMI 594 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 3550 AACGTT AA^CGTT PvuI 314 CGATCG CGAT^CG SacII 126 CCGCGG CCGC^GG SapI 560 GCTCTTC GCTCTTC(1/4) ScaI 3915 AGTACT AGT^ACT SfoI 197 GGCGCC GGC^GCC SfuI 642 TTCGAA TT^CGAA SnaBI 2052 TACGTA TAC^GTA SphI 2899 GCATGC GCATG^C Tth111I 3371 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2771 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 1782 GGTACC G^GTACC 3162 GGTACC AflII 1761 CTTAAG C^TTAAG 3692 CTTAAG AflIII 3573 ACATGT A^C[GA][CT]GT 3934 ACACGT AseI 2487 ATTAAT AT^TAAT 4212 ATTAAT AsnI 2487 ATTAAT AT^TAAT 4212 ATTAAT Asp718I 1782 GGTACC G^GTACC 3162 GGTACC BfrI 1761 CTTAAG C^TTAAG 3692 CTTAAG BsaAI 2052 TACGTA [CT]AC^GT[GA] 3480 CACGTG BsaWI 220 TCCGGA [AT]^CCGG[AT] 3649 TCCGGT BsmI 2074 GCATTC GAATGC(1/-1) 2166 GCATTC BspMI 859 ACCTGC ACCTGC(4/8) 2188 ACCTGC BsrBI 4099 CCGCTC CCGCTC(-3/-3) 4288 CCGCTC BsrDI 1735 GCAATG GCAATG(2/0) 2600 GCAATG BssHII 166 GCGCGC G^CGCGC 168 GCGCGC BstAPI 2014 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2591 GCAAGATTTGC BstEII 364 GGTGACC G^GT[ACGT]ACC 1277 GGTCACC BstPI 364 GGTGACC G^GT[ACGT]ACC 1277 GGTCACC DraI 2435 TTTAAA TTT^AAA 2835 TTTAAA DraIII 2025 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG 4689 CACGATGTG EcoNI 1520 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3325 CCTTGGAGAGG EcoO65I 364 GGTGACC G^GT[ACGT]ACC 1277 GGTCACC HindIII 2046 AAGCTT A^AGCTT 3382 AAGCTT KpnI 1782 GGTACC GGTAC^C 3162 GGTACC MfeI 1441 CAATTG C^AATTG 4297 CAATTG MunI 1441 CAATTG C^AATTG 4297 CAATTG NotI 65 GCGGCCGC GC^GGCCGC 133 GCGGCCGC PshAI 226 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4515 GACACCTGTC PshBI 2487 ATTAAT AT^TAAT 4212 ATTAAT PstI 39 CTGCAG CTGCA^G 4171 CTGCAG SacI 2230 GAGCTC GAGCT^C 3584 GAGCTC SmaI 255 CCCGGG CCC^GGG 754 CCCGGG TfiI 3093 GATTC G^A[AT]TC 4203 GAATC XmaCI 255 CCCGGG C^CCGGG 754 CCCGGG XmaI 255 CCCGGG C^CCGGG 754 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII XmnI