##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AgeI AhdI AscI AspEI
AviII AvrII BamHI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsiWI
Bsp1407I BspDI BspHI BsrGI BssSI
Bst1107I BstZ17I CelII ClaI CpoI
Eam1105I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HpaI MluI
NdeI NheI NruI NsiI PacI
PinAI PmeI RcaI RsrII SalI
SbfI SexAI SfiI SgrAI SpeI
Sse8387I SspBI SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1368 GTAGAC GT^[AC][GT]AC
AccIII 220 TCCGGA T^CCGGA
AclI 3550 AACGTT AA^CGTT
Alw44I 911 GTGCAC G^TGCAC
AocI 3831 CCTAAGG CC^T[ACGT]AGG
ApaI 4254 GGGCCC GGGCC^C
ApaLI 911 GTGCAC G^TGCAC
AspI 3371 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 197 GGCGCC GGCGC^C
BbrPI 3480 CACGTG CAC^GTG
BciVI 3569 GGATAC GTATCC(6/5)
BclI 4074 TGATCA T^GATCA
BglII 4132 AGATCT A^GATCT
BsaBI 1910 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 220 TCCGGA T^CCGGA
BspEI 220 TCCGGA T^CCGGA
BspLU11I 3573 ACATGT A^CATGT
BstBI 642 TTCGAA TT^CGAA
Bsu36I 3831 CCTAAGG CC^T[ACGT]AGG
DrdI 1269 GACGGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3831 CCTAAGG CC^T[ACGT]AGG
FbaI 4074 TGATCA T^GATCA
KasI 197 GGCGCC G^GCGCC
KspI 126 CCGCGG CCGC^GG
MamI 1910 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 220 TCCGGA T^CCGGA
NarI 197 GGCGCC GG^CGCC
NspV 642 TTCGAA TT^CGAA
PaeR7I 2771 CTCGAG C^TCGAG
PflFI 3371 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3480 CACGTG CAC^GTG
PmlI 3480 CACGTG CAC^GTG
PpuMI 594 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 3550 AACGTT AA^CGTT
PvuI 314 CGATCG CGAT^CG
SacII 126 CCGCGG CCGC^GG
SapI 560 GCTCTTC GCTCTTC(1/4)
ScaI 3915 AGTACT AGT^ACT
SfoI 197 GGCGCC GGC^GCC
SfuI 642 TTCGAA TT^CGAA
SnaBI 2052 TACGTA TAC^GTA
SphI 2899 GCATGC GCATG^C
Tth111I 3371 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2771 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1782 GGTACC G^GTACC
3162 GGTACC
AflII 1761 CTTAAG C^TTAAG
3692 CTTAAG
AflIII 3573 ACATGT A^C[GA][CT]GT
3934 ACACGT
AseI 2487 ATTAAT AT^TAAT
4212 ATTAAT
AsnI 2487 ATTAAT AT^TAAT
4212 ATTAAT
Asp718I 1782 GGTACC G^GTACC
3162 GGTACC
BfrI 1761 CTTAAG C^TTAAG
3692 CTTAAG
BsaAI 2052 TACGTA [CT]AC^GT[GA]
3480 CACGTG
BsaWI 220 TCCGGA [AT]^CCGG[AT]
3649 TCCGGT
BsmI 2074 GCATTC GAATGC(1/-1)
2166 GCATTC
BspMI 859 ACCTGC ACCTGC(4/8)
2188 ACCTGC
BsrBI 4099 CCGCTC CCGCTC(-3/-3)
4288 CCGCTC
BsrDI 1735 GCAATG GCAATG(2/0)
2600 GCAATG
BssHII 166 GCGCGC G^CGCGC
168 GCGCGC
BstAPI 2014 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2591 GCAAGATTTGC
BstEII 364 GGTGACC G^GT[ACGT]ACC
1277 GGTCACC
BstPI 364 GGTGACC G^GT[ACGT]ACC
1277 GGTCACC
DraI 2435 TTTAAA TTT^AAA
2835 TTTAAA
DraIII 2025 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
4689 CACGATGTG
EcoNI 1520 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3325 CCTTGGAGAGG
EcoO65I 364 GGTGACC G^GT[ACGT]ACC
1277 GGTCACC
HindIII 2046 AAGCTT A^AGCTT
3382 AAGCTT
KpnI 1782 GGTACC GGTAC^C
3162 GGTACC
MfeI 1441 CAATTG C^AATTG
4297 CAATTG
MunI 1441 CAATTG C^AATTG
4297 CAATTG
NotI 65 GCGGCCGC GC^GGCCGC
133 GCGGCCGC
PshAI 226 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4515 GACACCTGTC
PshBI 2487 ATTAAT AT^TAAT
4212 ATTAAT
PstI 39 CTGCAG CTGCA^G
4171 CTGCAG
SacI 2230 GAGCTC GAGCT^C
3584 GAGCTC
SmaI 255 CCCGGG CCC^GGG
754 CCCGGG
TfiI 3093 GATTC G^A[AT]TC
4203 GAATC
XmaCI 255 CCCGGG C^CCGGG
754 CCCGGG
XmaI 255 CCCGGG C^CCGGG
754 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI