##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII BalI BanI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 1481 TCCGGA T^CCGGA
AhdI 2327 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2327 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2634 CCTAGG C^CTAGG
BbsI 2778 GTCTTC GAAGAC(2/6)
BglI 176 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2634 CCTAGG C^CTAGG
BpuAI 2778 GTCTTC GAAGAC(2/6)
BsaI 374 GAGACC GGTCTC(1/5)
BsaWI 1481 TCCGGA [AT]^CCGG[AT]
BseAI 1481 TCCGGA T^CCGGA
BsmBI 3214 CGTCTC CGTCTC(1/5)
BsmI 2296 GAATGC GAATGC(1/-1)
BspEI 1481 TCCGGA T^CCGGA
BspMI 2863 ACCTGC ACCTGC(4/8)
BsrBI 999 GAGCGG CCGCTC(-3/-3)
BsrDI 3203 CATTGC GCAATG(2/0)
BssHII 100 GCGCGC G^CGCGC
BstAPI 513 GCACGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2831 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2327 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2409 CCTCTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 920 GATATC GAT^ATC
HincII 844 GTTGAC GT[CT]^[GA]AC
HindII 844 GTTGAC GT[CT]^[GA]AC
MfeI 630 CAATTG C^AATTG
MroI 1481 TCCGGA T^CCGGA
MunI 630 CAATTG C^AATTG
PaeR7I 2446 CTCGAG C^TCGAG
PflMI 1714 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 455 GGGACCC [GA]G^G[AT]CC[CT]
PstI 2357 CTGCAG CTGCA^G
SapI 322 GAAGAGC GCTCTTC(1/4)
SmaI 61 CCCGGG CCC^GGG
Van91I 1714 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2446 CTCGAG C^TCGAG
XmaCI 61 CCCGGG C^CCGGG
XmaI 61 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 443 GAATTT [GA]^AATT[CT]
1792 GAATTT
AflIII 870 ACGTGT A^C[GA][CT]GT
1092 ACGTGT
ApoI 443 GAATTT [GA]^AATT[CT]
1792 GAATTT
AspHI 325 GAGCTC G[AT]GC[AT]^C
1299 GAGCTC
AspI 1889 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2414 GACAGGGTC
BamHI 65 GGATCC G^GATCC
1162 GGATCC
BciVI 934 GGATAC GTATCC(6/5)
621 GTATCC
BsiHKAI 325 GAGCTC G[AT]GC[AT]^C
1299 GAGCTC
HaeII 89 GGCGCT [GA]GCGC^[CT]
184 GGCGCT
HgaI 257 GACGC GACGC(5/10)
230 GCGTC
NspI 1147 GCATGT [GA]CATG^[CT]
2670 GCATGT
PflFI 1889 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2414 GACAGGGTC
SacI 325 GAGCTC GAGCT^C
1299 GAGCTC
StuI 909 AGGCCT AGG^CCT
2268 AGGCCT
Tth111I 1889 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2414 GACAGGGTC
XbaI 647 TCTAGA T^CTAGA
3019 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII