##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
AscI AspEI AviII AvrII BbvCI
BciVI BlnI BlpI Bpu1102I BsaBI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII CpoI
DraIII EagI Eam1105I EclXI Eco52I
Eco81I EcoRV FseI FspI HgaI
KspI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 3901 GGTACC G^GTACC
AccIII 2418 TCCGGA T^CCGGA
AcyI 96 GGCGCC G[GA]^CG[CT]C
AflII 608 CTTAAG C^TTAAG
Alw44I 1560 GTGCAC G^TGCAC
Aor51HI 3055 AGCGCT AGC^GCT
ApaLI 1560 GTGCAC G^TGCAC
AseI 2647 ATTAAT AT^TAAT
AsnI 2647 ATTAAT AT^TAAT
Asp718I 3901 GGTACC G^GTACC
AspI 2129 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 230 CTCGGG C^[CT]CG[GA]G
BalI 4182 TGGCCA TGG^CCA
BbeI 96 GGCGCC GGCGC^C
BbrPI 1511 CACGTG CAC^GTG
BcgI 222 GCAGTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 493 TGATCA T^GATCA
BfrI 608 CTTAAG C^TTAAG
BglI 1318 GCCATACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4236 AGATCT A^GATCT
BsaAI 1511 CACGTG [CT]AC^GT[GA]
BsaHI 96 GGCGCC G[GA]^CG[CT]C
BseAI 2418 TCCGGA T^CCGGA
BsiEI 167 CGACCG CG[GA][CT]^CG
BsmBI 2561 CGTCTC CGTCTC(1/5)
BsoBI 230 CTCGGG C^[CT]CG[GA]G
BspDI 4258 ATCGAT AT^CGAT
BspEI 2418 TCCGGA T^CCGGA
BspHI 4168 TCATGA T^CATGA
BsrDI 3881 GCAATG GCAATG(2/0)
BstAPI 2388 GCACACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2893 CCAAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 4258 ATCGAT AT^CGAT
DrdI 2129 GACCAAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 676 CCATGG C^C[GA][CT]GG
Eco47III 3055 AGCGCT AGC^GCT
FauI 138 GCGGG CCCGC(4/6)
FbaI 493 TGATCA T^GATCA
Hin1I 96 GGCGCC G[GA]^CG[CT]C
HincII 3278 GTTAAC GT[CT]^[GA]AC
HindII 3278 GTTAAC GT[CT]^[GA]AC
HpaI 3278 GTTAAC GTT^AAC
KasI 96 GGCGCC G^GCGCC
KpnI 3901 GGTACC GGTAC^C
MluNI 4182 TGGCCA TGG^CCA
MroI 2418 TCCGGA T^CCGGA
MscI 4182 TGGCCA TGG^CCA
MspA1I 2991 CAGCTG C[AC]G^C[GT]G
NarI 96 GGCGCC GG^CGCC
NcoI 676 CCATGG C^CATGG
PflFI 2129 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1511 CACGTG CAC^GTG
PmlI 1511 CACGTG CAC^GTG
PpuMI 1290 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2647 ATTAAT AT^TAAT
PstI 3 CTGCAG CTGCA^G
PvuII 2991 CAGCTG CAG^CTG
RcaI 4168 TCATGA T^CATGA
SacI 1205 GAGCTC GAGCT^C
SexAI 2614 ACCAGGT A^CC[AT]GGT
SfoI 96 GGCGCC GGC^GCC
SwaI 3366 ATTTAAAT ATTT^AAAT
Tth111I 2129 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 3502 GTCTAC GT^[AC][GT]AC
4318 GTAGAC
AflIII 1510 ACACGT A^C[GA][CT]GT
1512 ACGTGT
ApaI 79 GGGCCC GGGCC^C
3357 GGGCCC
BamHI 518 GGATCC G^GATCC
1738 GGATCC
BsaI 582 GAGACC GGTCTC(1/5)
2963 GAGACC
BsaWI 1143 ACCGGA [AT]^CCGG[AT]
2418 TCCGGA
BsiWI 1336 CGTACG C^GTACG
1591 CGTACG
BsrFI 2082 ACCGGC [GA]^CCGG[CT]
2215 ACCGGC
BstEII 347 GGTGACC G^GT[ACGT]ACC
822 GGTGACC
BstPI 347 GGTGACC G^GT[ACGT]ACC
822 GGTGACC
Cfr10I 2082 ACCGGC [GA]^CCGG[CT]
2215 ACCGGC
EaeI 2084 CGGCCA [CT]^GGCC[GA]
4182 TGGCCA
EcoNI 120 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3348 CCTTTTGGAGG
EcoO65I 347 GGTGACC G^GT[ACGT]ACC
822 GGTGACC
HaeII 96 GGCGCC [GA]GCGC^[CT]
3055 AGCGCT
PflMI 554 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3650 CCAGGCACTGG
ScaI 2724 AGTACT AGT^ACT
4359 AGTACT
SmlI 608 CTTAAG C^T[CT][GA]AG
930 CTTGAG
Van91I 554 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3650 CCAGGCACTGG
XcmI 187 CCAGGAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
736 CCAACTCAGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI