##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccII AclI AflII
Alw44I AocI ApaI ApaLI AscI
AspI AvrII BamHI BbeI BfrI
BlnI BsaBI BsiEI BsiWI BspDI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI HindIII HpaI
KasI KspI MamI MluI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 158 GGTACC G^GTACC
AccIII 301 TCCGGA T^CCGGA
AcyI 5286 GGCGTC G[GA]^CG[CT]C
AgeI 3340 ACCGGT A^CCGGT
Aor51HI 2581 AGCGCT AGC^GCT
AseI 1383 ATTAAT AT^TAAT
AsnI 1383 ATTAAT AT^TAAT
Asp718I 158 GGTACC G^GTACC
AspHI 1825 GAGCAC G[AT]GC[AT]^C
AviII 67 TGCGCA TGC^GCA
BbrPI 5181 CACGTG CAC^GTG
BcgI 4694 GCAATTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2506 TGATCA T^GATCA
BglI 2469 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3998 AGATCT A^GATCT
BsaAI 5181 CACGTG [CT]AC^GT[GA]
BsaHI 5286 GGCGTC G[GA]^CG[CT]C
BseAI 301 TCCGGA T^CCGGA
BsiHKAI 1825 GAGCAC G[AT]GC[AT]^C
BsmBI 4438 CGTCTC CGTCTC(1/5)
BsmI 2353 GAATGC GAATGC(1/-1)
BspEI 301 TCCGGA T^CCGGA
BspHI 2503 TCATGA T^CATGA
BsrBI 197 CCGCTC CCGCTC(-3/-3)
DraI 5132 TTTAAA TTT^AAA
Eco47III 2581 AGCGCT AGC^GCT
EcoRI 165 GAATTC G^AATTC
FauI 1150 CCCGC CCCGC(4/6)
FbaI 2506 TGATCA T^GATCA
FspI 67 TGCGCA TGC^GCA
HaeII 2581 AGCGCT [GA]GCGC^[CT]
Hin1I 5286 GGCGTC G[GA]^CG[CT]C
KpnI 158 GGTACC GGTAC^C
MroI 301 TCCGGA T^CCGGA
PinAI 3340 ACCGGT A^CCGGT
PmaCI 5181 CACGTG CAC^GTG
PmlI 5181 CACGTG CAC^GTG
PshBI 1383 ATTAAT AT^TAAT
RcaI 2503 TCATGA T^CATGA
SapI 1715 GAAGAGC GCTCTTC(1/4)
SmlI 693 CTCAAG C^T[CT][GA]AG
SspI 1380 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AhdI 698 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3198 GACTTCCGGTC
AspEI 698 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3198 GACTTCCGGTC
AvaI 4702 CTCGGG C^[CT]CG[GA]G
4968 CTCGGG
BlpI 260 GCTAAGC GC^T[ACGT]AGC
3655 GCTCAGC
Bpu1102I 260 GCTAAGC GC^T[ACGT]AGC
3655 GCTCAGC
BsoBI 4702 CTCGGG C^[CT]CG[GA]G
4968 CTCGGG
BspLU11I 2542 ACATGT A^CATGT
4901 ACATGT
BsrFI 3340 ACCGGT [GA]^CCGG[CT]
4238 ACCGGC
BssSI 2458 CACGAG CACGAG(-5/-1)
4588 CTCGTG
CelII 260 GCTAAGC GC^T[ACGT]AGC
3655 GCTCAGC
Cfr10I 3340 ACCGGT [GA]^CCGG[CT]
4238 ACCGGC
DraIII 2119 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4123 CACAGAGTG
DrdI 5058 GACATACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5335 GACAAAGGGGTC
Eam1105I 698 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3198 GACTTCCGGTC
MfeI 427 CAATTG C^AATTG
4695 CAATTG
MunI 427 CAATTG C^AATTG
4695 CAATTG
NdeI 4243 CATATG CA^TATG
4796 CATATG
SbfI 584 CCTGCAGG CCTGCA^GG
5280 CCTGCAGG
SphI 256 GCATGC GCATG^C
4918 GCATGC
Sse8387I 584 CCTGCAGG CCTGCA^GG
5280 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI