##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I BamHI BbeI BbrPI BcgI
BclI BsaI BsiWI Bsp1407I BsrGI
BssHII CpoI DraI DraIII Eco47III
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI MfeI MluI MslI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AccI 1357 GTATAC GT^[AC][GT]AC
AccIII 2498 TCCGGA T^CCGGA
AcsI 3238 GAATTC [GA]^AATT[CT]
AcyI 556 GACGCC G[GA]^CG[CT]C
AflII 1430 CTTAAG C^TTAAG
AflIII 3600 ACATGT A^C[GA][CT]GT
AhdI 40 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2379 GGATC GGATC(4/5)
ApoI 3238 GAATTC [GA]^AATT[CT]
Asp700I 780 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 40 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1626 TGCGCA TGC^GCA
AvrII 2402 CCTAGG C^CTAGG
BalI 968 TGGCCA TGG^CCA
BbsI 2953 GAAGAC GAAGAC(2/6)
BciVI 1528 GGATAC GTATCC(6/5)
BfrI 1430 CTTAAG C^TTAAG
BglII 1191 AGATCT A^GATCT
BlnI 2402 CCTAGG C^CTAGG
BpuAI 2953 GAAGAC GAAGAC(2/6)
BsaAI 2703 TACGTG [CT]AC^GT[GA]
BsaBI 2708 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 556 GACGCC G[GA]^CG[CT]C
BseAI 2498 TCCGGA T^CCGGA
BspDI 2381 ATCGAT AT^CGAT
BspEI 2498 TCCGGA T^CCGGA
BspLU11I 3600 ACATGT A^CATGT
BsrDI 2460 GCAATG GCAATG(2/0)
BssSI 310 CTCGTG CACGAG(-5/-1)
Bst1107I 1357 GTATAC GTA^TAC
BstAPI 916 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1243 GGTGACC G^GT[ACGT]ACC
BstPI 1243 GGTGACC G^GT[ACGT]ACC
BstXI 86 CCATCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1191 AGATCT [GA]^GATC[CT]
BstZ17I 1357 GTATAC GTA^TAC
ClaI 2381 ATCGAT AT^CGAT
DrdI 375 GACGACGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 436 CGGCCG C^GGCCG
Eam1105I 40 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 436 CGGCCG C^GGCCG
Eco52I 436 CGGCCG C^GGCCG
EcoO65I 1243 GGTGACC G^GT[ACGT]ACC
EcoRI 3238 GAATTC G^AATTC
EcoRV 2633 GATATC GAT^ATC
FspI 1626 TGCGCA TGC^GCA
HaeII 1477 AGCGCC [GA]GCGC^[CT]
Hin1I 556 GACGCC G[GA]^CG[CT]C
HincII 2095 GTTGAC GT[CT]^[GA]AC
HindII 2095 GTTGAC GT[CT]^[GA]AC
KspI 328 CCGCGG CCGC^GG
MamI 2708 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1191 AGATCT [GA]^GATC[CT]
MluNI 968 TGGCCA TGG^CCA
MroI 2498 TCCGGA T^CCGGA
MscI 968 TGGCCA TGG^CCA
NspI 3600 ACATGT [GA]CATG^[CT]
PshAI 345 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 651 CAGCTG CAG^CTG
SacII 328 CCGCGG CCGC^GG
SgrAI 355 CACCGGCG C[GA]^CCGG[CT]G
SmaI 596 CCCGGG CCC^GGG
SspI 3616 AATATT AAT^ATT
StuI 3320 AGGCCT AGG^CCT
XhoII 1191 AGATCT [GA]^GATC[CT]
XmaCI 596 CCCGGG C^CCGGG
XmaI 596 CCCGGG C^CCGGG
XmnI 780 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 118 CGCG CG^CG
329 CGCG
AocI 671 CCTGAGG CC^T[ACGT]AGG
869 CCTGAGG
BanI 1321 GGTGCC G^G[CT][GA]CC
2407 GGCACC
BbvCI 1864 CCTCAGC CCTCAGC(-5/-2)
2420 CCTCAGC
BlpI 653 GCTGAGC GC^T[ACGT]AGC
1487 GCTCAGC
Bpu1102I 653 GCTGAGC GC^T[ACGT]AGC
1487 GCTCAGC
BsaWI 2498 TCCGGA [AT]^CCGG[AT]
3116 ACCGGA
BsmBI 47 CGTCTC CGTCTC(1/5)
2990 CGTCTC
BsmI 724 GAATGC GAATGC(1/-1)
1441 GCATTC
BspHI 1828 TCATGA T^CATGA
2266 TCATGA
BspMI 921 ACCTGC ACCTGC(4/8)
2444 GCAGGT
BsrBI 123 CCGCTC CCGCTC(-3/-3)
656 GAGCGG
BstBI 777 TTCGAA TT^CGAA
2796 TTCGAA
BstUI 118 CGCG CG^CG
329 CGCG
Bsu36I 671 CCTGAGG CC^T[ACGT]AGG
869 CCTGAGG
CelII 653 GCTGAGC GC^T[ACGT]AGC
1487 GCTCAGC
DpnII 1192 GATC ^GATC
2380 GATC
Eco81I 671 CCTGAGG CC^T[ACGT]AGG
869 CCTGAGG
MboI 1192 GATC ^GATC
2380 GATC
MvnI 118 CGCG CG^CG
329 CGCG
NaeI 434 GCCGGC GCC^GGC
438 GCCGGC
NcoI 1857 CCATGG C^CATGG
3449 CCATGG
NdeII 1192 GATC ^GATC
2380 GATC
NgoMIV 434 GCCGGC G^CCGGC
438 GCCGGC
NspV 777 TTCGAA TT^CGAA
2796 TTCGAA
PpuMI 343 GGGACCC [GA]G^G[AT]CC[CT]
2059 AGGACCT
RcaI 1828 TCATGA T^CATGA
2266 TCATGA
SacI 1696 GAGCTC GAGCT^C
3470 GAGCTC
Sau3AI 1192 GATC ^GATC
2380 GATC
SfuI 777 TTCGAA TT^CGAA
2796 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AspI AvaI AvaII BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PflFI PleI PstI RsaI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI