################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I BamHI BbeI BbrPI BcgI BclI BsaI BsiWI Bsp1407I BsrGI BssHII CpoI DraI DraIII Eco47III EcoT22I FbaI FseI HindIII HpaI KasI KpnI MfeI MluI MslI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AccI 1357 GTATAC GT^[AC][GT]AC AccIII 2498 TCCGGA T^CCGGA AcsI 3238 GAATTC [GA]^AATT[CT] AcyI 556 GACGCC G[GA]^CG[CT]C AflII 1430 CTTAAG C^TTAAG AflIII 3600 ACATGT A^C[GA][CT]GT AhdI 40 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 2379 GGATC GGATC(4/5) ApoI 3238 GAATTC [GA]^AATT[CT] Asp700I 780 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 40 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1626 TGCGCA TGC^GCA AvrII 2402 CCTAGG C^CTAGG BalI 968 TGGCCA TGG^CCA BbsI 2953 GAAGAC GAAGAC(2/6) BciVI 1528 GGATAC GTATCC(6/5) BfrI 1430 CTTAAG C^TTAAG BglII 1191 AGATCT A^GATCT BlnI 2402 CCTAGG C^CTAGG BpuAI 2953 GAAGAC GAAGAC(2/6) BsaAI 2703 TACGTG [CT]AC^GT[GA] BsaBI 2708 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 556 GACGCC G[GA]^CG[CT]C BseAI 2498 TCCGGA T^CCGGA BspDI 2381 ATCGAT AT^CGAT BspEI 2498 TCCGGA T^CCGGA BspLU11I 3600 ACATGT A^CATGT BsrDI 2460 GCAATG GCAATG(2/0) BssSI 310 CTCGTG CACGAG(-5/-1) Bst1107I 1357 GTATAC GTA^TAC BstAPI 916 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1243 GGTGACC G^GT[ACGT]ACC BstPI 1243 GGTGACC G^GT[ACGT]ACC BstXI 86 CCATCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1191 AGATCT [GA]^GATC[CT] BstZ17I 1357 GTATAC GTA^TAC ClaI 2381 ATCGAT AT^CGAT DrdI 375 GACGACGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 436 CGGCCG C^GGCCG Eam1105I 40 GACCCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 436 CGGCCG C^GGCCG Eco52I 436 CGGCCG C^GGCCG EcoO65I 1243 GGTGACC G^GT[ACGT]ACC EcoRI 3238 GAATTC G^AATTC EcoRV 2633 GATATC GAT^ATC FspI 1626 TGCGCA TGC^GCA HaeII 1477 AGCGCC [GA]GCGC^[CT] Hin1I 556 GACGCC G[GA]^CG[CT]C HincII 2095 GTTGAC GT[CT]^[GA]AC HindII 2095 GTTGAC GT[CT]^[GA]AC KspI 328 CCGCGG CCGC^GG MamI 2708 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1191 AGATCT [GA]^GATC[CT] MluNI 968 TGGCCA TGG^CCA MroI 2498 TCCGGA T^CCGGA MscI 968 TGGCCA TGG^CCA NspI 3600 ACATGT [GA]CATG^[CT] PshAI 345 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 651 CAGCTG CAG^CTG SacII 328 CCGCGG CCGC^GG SgrAI 355 CACCGGCG C[GA]^CCGG[CT]G SmaI 596 CCCGGG CCC^GGG SspI 3616 AATATT AAT^ATT StuI 3320 AGGCCT AGG^CCT XhoII 1191 AGATCT [GA]^GATC[CT] XmaCI 596 CCCGGG C^CCGGG XmaI 596 CCCGGG C^CCGGG XmnI 780 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 118 CGCG CG^CG 329 CGCG AocI 671 CCTGAGG CC^T[ACGT]AGG 869 CCTGAGG BanI 1321 GGTGCC G^G[CT][GA]CC 2407 GGCACC BbvCI 1864 CCTCAGC CCTCAGC(-5/-2) 2420 CCTCAGC BlpI 653 GCTGAGC GC^T[ACGT]AGC 1487 GCTCAGC Bpu1102I 653 GCTGAGC GC^T[ACGT]AGC 1487 GCTCAGC BsaWI 2498 TCCGGA [AT]^CCGG[AT] 3116 ACCGGA BsmBI 47 CGTCTC CGTCTC(1/5) 2990 CGTCTC BsmI 724 GAATGC GAATGC(1/-1) 1441 GCATTC BspHI 1828 TCATGA T^CATGA 2266 TCATGA BspMI 921 ACCTGC ACCTGC(4/8) 2444 GCAGGT BsrBI 123 CCGCTC CCGCTC(-3/-3) 656 GAGCGG BstBI 777 TTCGAA TT^CGAA 2796 TTCGAA BstUI 118 CGCG CG^CG 329 CGCG Bsu36I 671 CCTGAGG CC^T[ACGT]AGG 869 CCTGAGG CelII 653 GCTGAGC GC^T[ACGT]AGC 1487 GCTCAGC DpnII 1192 GATC ^GATC 2380 GATC Eco81I 671 CCTGAGG CC^T[ACGT]AGG 869 CCTGAGG MboI 1192 GATC ^GATC 2380 GATC MvnI 118 CGCG CG^CG 329 CGCG NaeI 434 GCCGGC GCC^GGC 438 GCCGGC NcoI 1857 CCATGG C^CATGG 3449 CCATGG NdeII 1192 GATC ^GATC 2380 GATC NgoMIV 434 GCCGGC G^CCGGC 438 GCCGGC NspV 777 TTCGAA TT^CGAA 2796 TTCGAA PpuMI 343 GGGACCC [GA]G^G[AT]CC[CT] 2059 AGGACCT RcaI 1828 TCATGA T^CATGA 2266 TCATGA SacI 1696 GAGCTC GAGCT^C 3470 GAGCTC Sau3AI 1192 GATC ^GATC 2380 GATC SfuI 777 TTCGAA TT^CGAA 2796 TTCGAA ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AspI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PflFI PleI PstI RsaI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI