################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AatII AclI AgeI Alw44I Aor51HI ApaLI AscI AviII AvrII BlnI BsaBI BspDI BspHI BssSI BstBI BstEII BstPI ClaI DrdI Eco47III EcoO65I EcoRI FspI HpaI KspI MamI MluI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI SacII SalI ScaI SexAI SfiI SfuI SgrAI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1957 GGTACC G^GTACC AccIII 108 TCCGGA T^CCGGA AseI 2620 ATTAAT AT^TAAT AsnI 2620 ATTAAT AT^TAAT Asp700I 3407 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1957 GGTACC G^GTACC BalI 311 TGGCCA TGG^CCA BamHI 4130 GGATCC G^GATCC BbeI 2045 GGCGCC GGCGC^C BbrPI 4660 CACGTG CAC^GTG BcgI 199 GCAAGTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 970 TGATCA T^GATCA BglII 946 AGATCT A^GATCT BseAI 108 TCCGGA T^CCGGA BsiWI 240 CGTACG C^GTACG Bsp1407I 4945 TGTACA T^GTACA BspEI 108 TCCGGA T^CCGGA BspLU11I 4496 ACATGT A^CATGT BsrBI 73 CCGCTC CCGCTC(-3/-3) BsrGI 4945 TGTACA T^GTACA Bst1107I 1451 GTATAC GTA^TAC BstZ17I 1451 GTATAC GTA^TAC CpoI 155 CGGTCCG CG^G[AT]CCG DraIII 873 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 2102 GATATC GAT^ATC FbaI 970 TGATCA T^GATCA HincII 2894 GTCAAC GT[CT]^[GA]AC HindII 2894 GTCAAC GT[CT]^[GA]AC HindIII 2698 AAGCTT A^AGCTT KasI 2045 GGCGCC G^GCGCC KpnI 1957 GGTACC GGTAC^C MfeI 1244 CAATTG C^AATTG MluNI 311 TGGCCA TGG^CCA MroI 108 TCCGGA T^CCGGA MscI 311 TGGCCA TGG^CCA MunI 1244 CAATTG C^AATTG NarI 2045 GGCGCC GG^CGCC PmaCI 4660 CACGTG CAC^GTG PmlI 4660 CACGTG CAC^GTG PshAI 2739 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2620 ATTAAT AT^TAAT RsrII 155 CGGTCCG CG^G[AT]CCG SfoI 2045 GGCGCC GGC^GCC SmaI 1782 CCCGGG CCC^GGG SnaBI 238 TACGTA TAC^GTA SspBI 4945 TGTACA T^GTACA XbaI 3808 TCTAGA T^CTAGA XcmI 2066 CCAGTGAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1782 CCCGGG C^CCGGG XmaI 1782 CCCGGG C^CCGGG XmnI 3407 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 1451 GTATAC GT^[AC][GT]AC 4841 GTAGAC AcsI 2200 AAATTC [GA]^AATT[CT] 2664 AAATTC AcyI 65 GGCGTC G[GA]^CG[CT]C 2045 GGCGCC AflIII 4496 ACATGT A^C[GA][CT]GT 4661 ACGTGT AhdI 2565 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3772 GACAGTGAGTC ApaI 231 GGGCCC GGGCC^C 4815 GGGCCC ApoI 2200 AAATTC [GA]^AATT[CT] 2664 AAATTC AspEI 2565 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3772 GACAGTGAGTC AspI 1487 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4227 GACTTTGTC AvaI 136 CTCGGG C^[CT]CG[GA]G 1782 CCCGGG BbsI 2354 GTCTTC GAAGAC(2/6) 2483 GTCTTC BpuAI 2354 GTCTTC GAAGAC(2/6) 2483 GTCTTC BsaAI 238 TACGTA [CT]AC^GT[GA] 4660 CACGTG BsaHI 65 GGCGTC G[GA]^CG[CT]C 2045 GGCGCC BsiEI 264 CGGCCG CG[GA][CT]^CG 521 CGGCCG BsmBI 67 CGTCTC CGTCTC(1/5) 323 GAGACG BsmI 893 GAATGC GAATGC(1/-1) 4378 GCATTC BsoBI 136 CTCGGG C^[CT]CG[GA]G 1782 CCCGGG BsrDI 1354 GCAATG GCAATG(2/0) 2556 GCAATG DraI 1419 TTTAAA TTT^AAA 4342 TTTAAA EagI 264 CGGCCG C^GGCCG 521 CGGCCG Eam1105I 2565 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3772 GACAGTGAGTC EclXI 264 CGGCCG C^GGCCG 521 CGGCCG Eco52I 264 CGGCCG C^GGCCG 521 CGGCCG EcoT22I 3723 ATGCAT ATGCA^T 3739 ATGCAT FseI 261 GGCCGGCC GGCCGG^CC 518 GGCCGGCC HgaI 325 GACGC GACGC(5/10) 66 GCGTC Hin1I 65 GGCGTC G[GA]^CG[CT]C 2045 GGCGCC MaeII 239 ACGT A^CGT 4661 ACGT NaeI 262 GCCGGC GCC^GGC 519 GCCGGC NgoMIV 262 GCCGGC G^CCGGC 519 GCCGGC NsiI 3723 ATGCAT ATGCA^T 3739 ATGCAT PflFI 1487 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4227 GACTTTGTC SapI 423 GCTCTTC GCTCTTC(1/4) 1681 GCTCTTC SbfI 836 CCTGCAGG CCTGCA^GG 4488 CCTGCAGG Sse8387I 836 CCTGCAGG CCTGCA^GG 4488 CCTGCAGG StuI 464 AGGCCT AGG^CCT 751 AGGCCT TaqI 1435 TCGA T^CGA 2537 TCGA Tth111I 1487 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4227 GACTTTGTC TthHB8I 1435 TCGA T^CGA 2537 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AfaI AflII AluI AlwI AlwNI AocI AspHI AvaII BanI BanII BbvCI BbvI BciVI BcnI BfaI BfrI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII