##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AgeI Alw44I Aor51HI
ApaLI AscI AviII AvrII BlnI
BsaBI BspDI BspHI BssSI BstBI
BstEII BstPI ClaI DrdI Eco47III
EcoO65I EcoRI FspI HpaI KspI
MamI MluI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RcaI SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1957 GGTACC G^GTACC
AccIII 108 TCCGGA T^CCGGA
AseI 2620 ATTAAT AT^TAAT
AsnI 2620 ATTAAT AT^TAAT
Asp700I 3407 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1957 GGTACC G^GTACC
BalI 311 TGGCCA TGG^CCA
BamHI 4130 GGATCC G^GATCC
BbeI 2045 GGCGCC GGCGC^C
BbrPI 4660 CACGTG CAC^GTG
BcgI 199 GCAAGTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 970 TGATCA T^GATCA
BglII 946 AGATCT A^GATCT
BseAI 108 TCCGGA T^CCGGA
BsiWI 240 CGTACG C^GTACG
Bsp1407I 4945 TGTACA T^GTACA
BspEI 108 TCCGGA T^CCGGA
BspLU11I 4496 ACATGT A^CATGT
BsrBI 73 CCGCTC CCGCTC(-3/-3)
BsrGI 4945 TGTACA T^GTACA
Bst1107I 1451 GTATAC GTA^TAC
BstZ17I 1451 GTATAC GTA^TAC
CpoI 155 CGGTCCG CG^G[AT]CCG
DraIII 873 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 2102 GATATC GAT^ATC
FbaI 970 TGATCA T^GATCA
HincII 2894 GTCAAC GT[CT]^[GA]AC
HindII 2894 GTCAAC GT[CT]^[GA]AC
HindIII 2698 AAGCTT A^AGCTT
KasI 2045 GGCGCC G^GCGCC
KpnI 1957 GGTACC GGTAC^C
MfeI 1244 CAATTG C^AATTG
MluNI 311 TGGCCA TGG^CCA
MroI 108 TCCGGA T^CCGGA
MscI 311 TGGCCA TGG^CCA
MunI 1244 CAATTG C^AATTG
NarI 2045 GGCGCC GG^CGCC
PmaCI 4660 CACGTG CAC^GTG
PmlI 4660 CACGTG CAC^GTG
PshAI 2739 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2620 ATTAAT AT^TAAT
RsrII 155 CGGTCCG CG^G[AT]CCG
SfoI 2045 GGCGCC GGC^GCC
SmaI 1782 CCCGGG CCC^GGG
SnaBI 238 TACGTA TAC^GTA
SspBI 4945 TGTACA T^GTACA
XbaI 3808 TCTAGA T^CTAGA
XcmI 2066 CCAGTGAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1782 CCCGGG C^CCGGG
XmaI 1782 CCCGGG C^CCGGG
XmnI 3407 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1451 GTATAC GT^[AC][GT]AC
4841 GTAGAC
AcsI 2200 AAATTC [GA]^AATT[CT]
2664 AAATTC
AcyI 65 GGCGTC G[GA]^CG[CT]C
2045 GGCGCC
AflIII 4496 ACATGT A^C[GA][CT]GT
4661 ACGTGT
AhdI 2565 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3772 GACAGTGAGTC
ApaI 231 GGGCCC GGGCC^C
4815 GGGCCC
ApoI 2200 AAATTC [GA]^AATT[CT]
2664 AAATTC
AspEI 2565 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3772 GACAGTGAGTC
AspI 1487 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4227 GACTTTGTC
AvaI 136 CTCGGG C^[CT]CG[GA]G
1782 CCCGGG
BbsI 2354 GTCTTC GAAGAC(2/6)
2483 GTCTTC
BpuAI 2354 GTCTTC GAAGAC(2/6)
2483 GTCTTC
BsaAI 238 TACGTA [CT]AC^GT[GA]
4660 CACGTG
BsaHI 65 GGCGTC G[GA]^CG[CT]C
2045 GGCGCC
BsiEI 264 CGGCCG CG[GA][CT]^CG
521 CGGCCG
BsmBI 67 CGTCTC CGTCTC(1/5)
323 GAGACG
BsmI 893 GAATGC GAATGC(1/-1)
4378 GCATTC
BsoBI 136 CTCGGG C^[CT]CG[GA]G
1782 CCCGGG
BsrDI 1354 GCAATG GCAATG(2/0)
2556 GCAATG
DraI 1419 TTTAAA TTT^AAA
4342 TTTAAA
EagI 264 CGGCCG C^GGCCG
521 CGGCCG
Eam1105I 2565 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3772 GACAGTGAGTC
EclXI 264 CGGCCG C^GGCCG
521 CGGCCG
Eco52I 264 CGGCCG C^GGCCG
521 CGGCCG
EcoT22I 3723 ATGCAT ATGCA^T
3739 ATGCAT
FseI 261 GGCCGGCC GGCCGG^CC
518 GGCCGGCC
HgaI 325 GACGC GACGC(5/10)
66 GCGTC
Hin1I 65 GGCGTC G[GA]^CG[CT]C
2045 GGCGCC
MaeII 239 ACGT A^CGT
4661 ACGT
NaeI 262 GCCGGC GCC^GGC
519 GCCGGC
NgoMIV 262 GCCGGC G^CCGGC
519 GCCGGC
NsiI 3723 ATGCAT ATGCA^T
3739 ATGCAT
PflFI 1487 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4227 GACTTTGTC
SapI 423 GCTCTTC GCTCTTC(1/4)
1681 GCTCTTC
SbfI 836 CCTGCAGG CCTGCA^GG
4488 CCTGCAGG
Sse8387I 836 CCTGCAGG CCTGCA^GG
4488 CCTGCAGG
StuI 464 AGGCCT AGG^CCT
751 AGGCCT
TaqI 1435 TCGA T^CGA
2537 TCGA
Tth111I 1487 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4227 GACTTTGTC
TthHB8I 1435 TCGA T^CGA
2537 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AfaI AflII AluI
AlwI AlwNI AocI AspHI AvaII
BanI BanII BbvCI BbvI BciVI
BcnI BfaI BfrI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII