##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
BcgI BciVI BclI BfrI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
DraI EcoRI EcoRV FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NotI NruI
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3016 GTCTAC GT^[AC][GT]AC
AhdI 1313 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1101 GTGCAC G^TGCAC
ApaLI 1101 GTGCAC G^TGCAC
AspEI 1313 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1218 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1366 CCTAGG C^CTAGG
BamHI 313 GGATCC G^GATCC
BbeI 1713 GGCGCC GGCGC^C
BglII 3576 AGATCT A^GATCT
BlnI 1366 CCTAGG C^CTAGG
BlpI 1725 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1725 GCTCAGC GC^T[ACGT]AGC
BsmBI 54 GAGACG CGTCTC(1/5)
BspHI 3990 TCATGA T^CATGA
BsrFI 1387 ACCGGC [GA]^CCGG[CT]
BstBI 2599 TTCGAA TT^CGAA
CelII 1725 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1387 ACCGGC [GA]^CCGG[CT]
EagI 1389 CGGCCG C^GGCCG
Eam1105I 1313 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1389 CGGCCG C^GGCCG
Eco52I 1389 CGGCCG C^GGCCG
EcoT22I 495 ATGCAT ATGCA^T
HincII 3915 GTTGAC GT[CT]^[GA]AC
HindII 3915 GTTGAC GT[CT]^[GA]AC
HindIII 1592 AAGCTT A^AGCTT
KasI 1713 GGCGCC G^GCGCC
MseI 3981 TTAA T^TAA
MslI 3807 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1713 GGCGCC GG^CGCC
NheI 3382 GCTAGC G^CTAGC
NsiI 495 ATGCAT ATGCA^T
NspV 2599 TTCGAA TT^CGAA
PaeR7I 18 CTCGAG C^TCGAG
PflFI 1218 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3990 TCATGA T^CATGA
SfoI 1713 GGCGCC GGC^GCC
SfuI 2599 TTCGAA TT^CGAA
Tru9I 3981 TTAA T^TAA
Tsp509I 3974 AATT ^AATT
Tth111I 1218 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 18 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 1014 GGGCCC GGGCC^C
3722 GGGCCC
AspHI 1101 GTGCAC G[AT]GC[AT]^C
2354 GTGCTC
BanI 1713 GGCGCC G^G[CT][GA]CC
1779 GGCACC
BbrPI 1293 CACGTG CAC^GTG
1581 CACGTG
BbvCI 389 GCTGAGG CCTCAGC(-5/-2)
1052 GCTGAGG
BsaAI 1293 CACGTG [CT]AC^GT[GA]
1581 CACGTG
BsaI 144 GAGACC GGTCTC(1/5)
2166 GGTCTC
BsiEI 1389 CGGCCG CG[GA][CT]^CG
3670 CGGTCG
BsiHKAI 1101 GTGCAC G[AT]GC[AT]^C
2354 GTGCTC
BstAPI 1289 GCACCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2696 GCAGCAAGTGC
BstEII 1682 GGTCACC G^GT[ACGT]ACC
3265 GGTGACC
BstPI 1682 GGTCACC G^GT[ACGT]ACC
3265 GGTGACC
DraIII 1290 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1767 CACGACGTG
DrdI 1674 GACGTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2879 GACACTGGGGTC
DsaI 759 CCACGG C^C[GA][CT]GG
1608 CCACGG
EcoO65I 1682 GGTCACC G^GT[ACGT]ACC
3265 GGTGACC
NspI 372 ACATGC [GA]CATG^[CT]
1327 ACATGC
PmaCI 1293 CACGTG CAC^GTG
1581 CACGTG
PmlI 1293 CACGTG CAC^GTG
1581 CACGTG
SfiI 1006 GGCCGAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2500 GGCCAAGGTGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI