################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI ApoI AscI AseI AsnI Asp700I Asp718I AviII BcgI BciVI BclI BfrI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstZ17I ClaI CpoI DraI EcoRI EcoRV FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NotI NruI PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 3016 GTCTAC GT^[AC][GT]AC AhdI 1313 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1101 GTGCAC G^TGCAC ApaLI 1101 GTGCAC G^TGCAC AspEI 1313 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1218 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1366 CCTAGG C^CTAGG BamHI 313 GGATCC G^GATCC BbeI 1713 GGCGCC GGCGC^C BglII 3576 AGATCT A^GATCT BlnI 1366 CCTAGG C^CTAGG BlpI 1725 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1725 GCTCAGC GC^T[ACGT]AGC BsmBI 54 GAGACG CGTCTC(1/5) BspHI 3990 TCATGA T^CATGA BsrFI 1387 ACCGGC [GA]^CCGG[CT] BstBI 2599 TTCGAA TT^CGAA CelII 1725 GCTCAGC GC^T[ACGT]AGC Cfr10I 1387 ACCGGC [GA]^CCGG[CT] EagI 1389 CGGCCG C^GGCCG Eam1105I 1313 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1389 CGGCCG C^GGCCG Eco52I 1389 CGGCCG C^GGCCG EcoT22I 495 ATGCAT ATGCA^T HincII 3915 GTTGAC GT[CT]^[GA]AC HindII 3915 GTTGAC GT[CT]^[GA]AC HindIII 1592 AAGCTT A^AGCTT KasI 1713 GGCGCC G^GCGCC MseI 3981 TTAA T^TAA MslI 3807 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1713 GGCGCC GG^CGCC NheI 3382 GCTAGC G^CTAGC NsiI 495 ATGCAT ATGCA^T NspV 2599 TTCGAA TT^CGAA PaeR7I 18 CTCGAG C^TCGAG PflFI 1218 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 3990 TCATGA T^CATGA SfoI 1713 GGCGCC GGC^GCC SfuI 2599 TTCGAA TT^CGAA Tru9I 3981 TTAA T^TAA Tsp509I 3974 AATT ^AATT Tth111I 1218 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 18 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### ApaI 1014 GGGCCC GGGCC^C 3722 GGGCCC AspHI 1101 GTGCAC G[AT]GC[AT]^C 2354 GTGCTC BanI 1713 GGCGCC G^G[CT][GA]CC 1779 GGCACC BbrPI 1293 CACGTG CAC^GTG 1581 CACGTG BbvCI 389 GCTGAGG CCTCAGC(-5/-2) 1052 GCTGAGG BsaAI 1293 CACGTG [CT]AC^GT[GA] 1581 CACGTG BsaI 144 GAGACC GGTCTC(1/5) 2166 GGTCTC BsiEI 1389 CGGCCG CG[GA][CT]^CG 3670 CGGTCG BsiHKAI 1101 GTGCAC G[AT]GC[AT]^C 2354 GTGCTC BstAPI 1289 GCACCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2696 GCAGCAAGTGC BstEII 1682 GGTCACC G^GT[ACGT]ACC 3265 GGTGACC BstPI 1682 GGTCACC G^GT[ACGT]ACC 3265 GGTGACC DraIII 1290 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 1767 CACGACGTG DrdI 1674 GACGTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2879 GACACTGGGGTC DsaI 759 CCACGG C^C[GA][CT]GG 1608 CCACGG EcoO65I 1682 GGTCACC G^GT[ACGT]ACC 3265 GGTGACC NspI 372 ACATGC [GA]CATG^[CT] 1327 ACATGC PmaCI 1293 CACGTG CAC^GTG 1581 CACGTG PmlI 1293 CACGTG CAC^GTG 1581 CACGTG SfiI 1006 GGCCGAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2500 GGCCAAGGTGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI