##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AhdI Aor51HI AscI AseI
AsnI AspEI AvrII BbeI BbrPI
BclI BfrI BlnI BsaBI BsaHI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI BstBI BstUI ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FbaI
HaeII Hin1I KasI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NdeI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SexAI
SfoI SfuI SpeI SphI SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 4645 GGTACC G^GTACC
AccI 3168 GTATAC GT^[AC][GT]AC
AcsI 3675 AAATTT [GA]^AATT[CT]
AgeI 3939 ACCGGT A^CCGGT
Alw44I 479 GTGCAC G^TGCAC
ApaLI 479 GTGCAC G^TGCAC
ApoI 3675 AAATTT [GA]^AATT[CT]
Asp718I 4645 GGTACC G^GTACC
AspI 3339 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2746 TGCGCA TGC^GCA
BamHI 817 GGATCC G^GATCC
BcgI 44 GCAGCCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 612 AGATCT A^GATCT
BsaI 1813 GGTCTC GGTCTC(1/5)
BsmBI 3889 GAGACG CGTCTC(1/5)
Bst1107I 3168 GTATAC GTA^TAC
BstZ17I 3168 GTATAC GTA^TAC
DraIII 2081 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2994 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2436 ATGCAT ATGCA^T
FseI 2798 GGCCGGCC GGCCGG^CC
FspI 2746 TGCGCA TGC^GCA
HgaI 2723 GACGC GACGC(5/10)
HpaI 2958 GTTAAC GTT^AAC
KpnI 4645 GGTACC GGTAC^C
MseI 2959 TTAA T^TAA
NsiI 2436 ATGCAT ATGCA^T
PflFI 3339 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3939 ACCGGT A^CCGGT
PpuMI 3337 GGGACCC [GA]G^G[AT]CC[CT]
SapI 1175 GAAGAGC GCTCTTC(1/4)
SbfI 1436 CCTGCAGG CCTGCA^GG
SfiI 975 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3938 CACCGGTG C[GA]^CCGG[CT]G
SmaI 822 CCCGGG CCC^GGG
SnaBI 3520 TACGTA TAC^GTA
Sse8387I 1436 CCTGCAGG CCTGCA^GG
TaqI 1971 TCGA T^CGA
Tru9I 2959 TTAA T^TAA
Tth111I 3339 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1971 TCGA T^CGA
XmaCI 822 CCCGGG C^CCGGG
XmaI 822 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 3164 ACATGT A^C[GA][CT]GT
4492 ACATGT
Asp700I 1133 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1414 GAAGGAGTTC
AvaI 822 CCCGGG C^[CT]CG[GA]G
2190 CCCGAG
BbvCI 2125 CCTCAGC CCTCAGC(-5/-2)
2144 GCTGAGG
BciVI 994 GGATAC GTATCC(6/5)
1778 GTATCC
BlpI 405 GCTCAGC GC^T[ACGT]AGC
525 GCTCAGC
Bpu1102I 405 GCTCAGC GC^T[ACGT]AGC
525 GCTCAGC
BsaAI 2858 TACGTG [CT]AC^GT[GA]
3520 TACGTA
BsaWI 2256 TCCGGT [AT]^CCGG[AT]
3939 ACCGGT
BsgI 4447 CTGCAC GTGCAG(16/14)
4716 CTGCAC
BsoBI 822 CCCGGG C^[CT]CG[GA]G
2190 CCCGAG
BspLU11I 3164 ACATGT A^CATGT
4492 ACATGT
BsrBI 4184 CCGCTC CCGCTC(-3/-3)
2421 GAGCGG
BstAPI 895 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4016 GCAGCGTGTGC
BstXI 762 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3326 CCATGTCACTGG
BstYI 612 AGATCT [GA]^GATC[CT]
817 GGATCC
CelII 405 GCTCAGC GC^T[ACGT]AGC
525 GCTCAGC
HincII 2958 GTTAAC GT[CT]^[GA]AC
4584 GTCAAC
HindII 2958 GTTAAC GT[CT]^[GA]AC
4584 GTCAAC
HindIII 1169 AAGCTT A^AGCTT
1641 AAGCTT
MflI 612 AGATCT [GA]^GATC[CT]
817 GGATCC
NaeI 1270 GCCGGC GCC^GGC
2799 GCCGGC
NgoMIV 1270 GCCGGC G^CCGGC
2799 GCCGGC
NheI 329 GCTAGC G^CTAGC
4458 GCTAGC
PflMI 1820 CCAATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3314 CCAGCTTATGG
ScaI 1455 AGTACT AGT^ACT
2906 AGTACT
SspI 1955 AATATT AAT^ATT
4203 AATATT
Tsp509I 3676 AATT ^AATT
4612 AATT
Van91I 1820 CCAATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3314 CCAGCTTATGG
XhoII 612 AGATCT [GA]^GATC[CT]
817 GGATCC
XmnI 1133 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1414 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI TseI Tsp45I TspRI
XcmI