################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AhdI Aor51HI AscI AseI AsnI AspEI AvrII BbeI BbrPI BclI BfrI BlnI BsaBI BsaHI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI BstBI BstUI ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI HaeII Hin1I KasI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SexAI SfoI SfuI SpeI SphI SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 4645 GGTACC G^GTACC AccI 3168 GTATAC GT^[AC][GT]AC AcsI 3675 AAATTT [GA]^AATT[CT] AgeI 3939 ACCGGT A^CCGGT Alw44I 479 GTGCAC G^TGCAC ApaLI 479 GTGCAC G^TGCAC ApoI 3675 AAATTT [GA]^AATT[CT] Asp718I 4645 GGTACC G^GTACC AspI 3339 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2746 TGCGCA TGC^GCA BamHI 817 GGATCC G^GATCC BcgI 44 GCAGCCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 612 AGATCT A^GATCT BsaI 1813 GGTCTC GGTCTC(1/5) BsmBI 3889 GAGACG CGTCTC(1/5) Bst1107I 3168 GTATAC GTA^TAC BstZ17I 3168 GTATAC GTA^TAC DraIII 2081 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2994 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 2436 ATGCAT ATGCA^T FseI 2798 GGCCGGCC GGCCGG^CC FspI 2746 TGCGCA TGC^GCA HgaI 2723 GACGC GACGC(5/10) HpaI 2958 GTTAAC GTT^AAC KpnI 4645 GGTACC GGTAC^C MseI 2959 TTAA T^TAA NsiI 2436 ATGCAT ATGCA^T PflFI 3339 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3939 ACCGGT A^CCGGT PpuMI 3337 GGGACCC [GA]G^G[AT]CC[CT] SapI 1175 GAAGAGC GCTCTTC(1/4) SbfI 1436 CCTGCAGG CCTGCA^GG SfiI 975 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 3938 CACCGGTG C[GA]^CCGG[CT]G SmaI 822 CCCGGG CCC^GGG SnaBI 3520 TACGTA TAC^GTA Sse8387I 1436 CCTGCAGG CCTGCA^GG TaqI 1971 TCGA T^CGA Tru9I 2959 TTAA T^TAA Tth111I 3339 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1971 TCGA T^CGA XmaCI 822 CCCGGG C^CCGGG XmaI 822 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AflIII 3164 ACATGT A^C[GA][CT]GT 4492 ACATGT Asp700I 1133 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1414 GAAGGAGTTC AvaI 822 CCCGGG C^[CT]CG[GA]G 2190 CCCGAG BbvCI 2125 CCTCAGC CCTCAGC(-5/-2) 2144 GCTGAGG BciVI 994 GGATAC GTATCC(6/5) 1778 GTATCC BlpI 405 GCTCAGC GC^T[ACGT]AGC 525 GCTCAGC Bpu1102I 405 GCTCAGC GC^T[ACGT]AGC 525 GCTCAGC BsaAI 2858 TACGTG [CT]AC^GT[GA] 3520 TACGTA BsaWI 2256 TCCGGT [AT]^CCGG[AT] 3939 ACCGGT BsgI 4447 CTGCAC GTGCAG(16/14) 4716 CTGCAC BsoBI 822 CCCGGG C^[CT]CG[GA]G 2190 CCCGAG BspLU11I 3164 ACATGT A^CATGT 4492 ACATGT BsrBI 4184 CCGCTC CCGCTC(-3/-3) 2421 GAGCGG BstAPI 895 GCATTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4016 GCAGCGTGTGC BstXI 762 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3326 CCATGTCACTGG BstYI 612 AGATCT [GA]^GATC[CT] 817 GGATCC CelII 405 GCTCAGC GC^T[ACGT]AGC 525 GCTCAGC HincII 2958 GTTAAC GT[CT]^[GA]AC 4584 GTCAAC HindII 2958 GTTAAC GT[CT]^[GA]AC 4584 GTCAAC HindIII 1169 AAGCTT A^AGCTT 1641 AAGCTT MflI 612 AGATCT [GA]^GATC[CT] 817 GGATCC NaeI 1270 GCCGGC GCC^GGC 2799 GCCGGC NgoMIV 1270 GCCGGC G^CCGGC 2799 GCCGGC NheI 329 GCTAGC G^CTAGC 4458 GCTAGC PflMI 1820 CCAATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3314 CCAGCTTATGG ScaI 1455 AGTACT AGT^ACT 2906 AGTACT SspI 1955 AATATT AAT^ATT 4203 AATATT Tsp509I 3676 AATT ^AATT 4612 AATT Van91I 1820 CCAATAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3314 CCAGCTTATGG XhoII 612 AGATCT [GA]^GATC[CT] 817 GGATCC XmnI 1133 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1414 GAAGGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI TseI Tsp45I TspRI XcmI