################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BbrPI BcgI BciVI BfrI BglII BlnI BsaAI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoT22I FseI HindIII KpnI MamI MluI NdeI NheI NotI NruI NsiI PacI PaeR7I PflMI PmaCI PmeI PmlI PshBI Psp1406I RsrII SalI SapI SbfI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 2047 TCCGGA T^CCGGA AgeI 103 ACCGGT A^CCGGT AspHI 194 GAGCTC G[AT]GC[AT]^C AspI 2805 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 235 CCCGAG C^[CT]CG[GA]G BamHI 2478 GGATCC G^GATCC BbeI 2495 GGCGCC GGCGC^C BbvCI 2019 CCTCAGC CCTCAGC(-5/-2) BclI 2128 TGATCA T^GATCA BseAI 2047 TCCGGA T^CCGGA BsiHKAI 194 GAGCTC G[AT]GC[AT]^C BsoBI 235 CCCGAG C^[CT]CG[GA]G BspEI 2047 TCCGGA T^CCGGA BsrBI 22 GAGCGG CCGCTC(-3/-3) BssHII 1029 GCGCGC G^CGCGC BstAPI 1481 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1624 TTCGAA TT^CGAA BstXI 3098 CCATCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 1347 GACATGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 513 GATATC GAT^ATC FbaI 2128 TGATCA T^GATCA HpaI 99 GTTAAC GTT^AAC KasI 2495 GGCGCC G^GCGCC KspI 71 CCGCGG CCGC^GG MfeI 912 CAATTG C^AATTG MroI 2047 TCCGGA T^CCGGA MunI 912 CAATTG C^AATTG NaeI 186 GCCGGC GCC^GGC NarI 2495 GGCGCC GG^CGCC NgoMIV 186 GCCGGC G^CCGGC NspV 1624 TTCGAA TT^CGAA PflFI 2805 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 103 ACCGGT A^CCGGT SacI 194 GAGCTC GAGCT^C SacII 71 CCGCGG CCGC^GG ScaI 1681 AGTACT AGT^ACT SexAI 2053 ACCTGGT A^CC[AT]GGT SfoI 2495 GGCGCC GGC^GCC SfuI 1624 TTCGAA TT^CGAA SmlI 621 CTCAAG C^T[CT][GA]AG Tth111I 2805 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 969 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 3146 CAGCAGCTG AocI 1922 CCTGAGG CC^T[ACGT]AGG 2614 CCTGAGG ApaI 48 GGGCCC GGGCC^C 2450 GGGCCC Asp700I 326 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1790 GAAGGGCTTC AviII 742 TGCGCA TGC^GCA 2248 TGCGCA BfaI 462 CTAG C^TAG 720 CTAG BlpI 1557 GCTCAGC GC^T[ACGT]AGC 2226 GCTCAGC Bpu1102I 1557 GCTCAGC GC^T[ACGT]AGC 2226 GCTCAGC BspHI 1660 TCATGA T^CATGA 2125 TCATGA Bsu36I 1922 CCTGAGG CC^T[ACGT]AGG 2614 CCTGAGG CelII 1557 GCTCAGC GC^T[ACGT]AGC 2226 GCTCAGC EarI 1266 CTCTTC CTCTTC(1/4) 1824 CTCTTC Eco81I 1922 CCTGAGG CC^T[ACGT]AGG 2614 CCTGAGG EcoNI 890 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1918 CCTTCCTGAGG FspI 742 TGCGCA TGC^GCA 2248 TGCGCA Ksp632I 1266 CTCTTC CTCTTC(1/4) 1824 CTCTTC MaeI 462 CTAG C^TAG 720 CTAG NcoI 61 CCATGG C^CATGG 1247 CCATGG PshAI 482 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2303 GACCACCGTC PvuI 377 CGATCG CGAT^CG 2635 CGATCG RcaI 1660 TCATGA T^CATGA 2125 TCATGA XcmI 592 CCAACTCGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2044 CCATCCGGAACCTGG XmnI 326 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1790 GAAGGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AvaII BalI BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII