##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvrII
BbrPI BcgI BciVI BfrI BglII
BlnI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I Eco47III EcoO65I EcoRI EcoT22I
FseI HindIII KpnI MamI MluI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshBI Psp1406I RsrII SalI
SapI SbfI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2047 TCCGGA T^CCGGA
AgeI 103 ACCGGT A^CCGGT
AspHI 194 GAGCTC G[AT]GC[AT]^C
AspI 2805 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 235 CCCGAG C^[CT]CG[GA]G
BamHI 2478 GGATCC G^GATCC
BbeI 2495 GGCGCC GGCGC^C
BbvCI 2019 CCTCAGC CCTCAGC(-5/-2)
BclI 2128 TGATCA T^GATCA
BseAI 2047 TCCGGA T^CCGGA
BsiHKAI 194 GAGCTC G[AT]GC[AT]^C
BsoBI 235 CCCGAG C^[CT]CG[GA]G
BspEI 2047 TCCGGA T^CCGGA
BsrBI 22 GAGCGG CCGCTC(-3/-3)
BssHII 1029 GCGCGC G^CGCGC
BstAPI 1481 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1624 TTCGAA TT^CGAA
BstXI 3098 CCATCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1347 GACATGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 513 GATATC GAT^ATC
FbaI 2128 TGATCA T^GATCA
HpaI 99 GTTAAC GTT^AAC
KasI 2495 GGCGCC G^GCGCC
KspI 71 CCGCGG CCGC^GG
MfeI 912 CAATTG C^AATTG
MroI 2047 TCCGGA T^CCGGA
MunI 912 CAATTG C^AATTG
NaeI 186 GCCGGC GCC^GGC
NarI 2495 GGCGCC GG^CGCC
NgoMIV 186 GCCGGC G^CCGGC
NspV 1624 TTCGAA TT^CGAA
PflFI 2805 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 103 ACCGGT A^CCGGT
SacI 194 GAGCTC GAGCT^C
SacII 71 CCGCGG CCGC^GG
ScaI 1681 AGTACT AGT^ACT
SexAI 2053 ACCTGGT A^CC[AT]GGT
SfoI 2495 GGCGCC GGC^GCC
SfuI 1624 TTCGAA TT^CGAA
SmlI 621 CTCAAG C^T[CT][GA]AG
Tth111I 2805 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 969 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3146 CAGCAGCTG
AocI 1922 CCTGAGG CC^T[ACGT]AGG
2614 CCTGAGG
ApaI 48 GGGCCC GGGCC^C
2450 GGGCCC
Asp700I 326 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1790 GAAGGGCTTC
AviII 742 TGCGCA TGC^GCA
2248 TGCGCA
BfaI 462 CTAG C^TAG
720 CTAG
BlpI 1557 GCTCAGC GC^T[ACGT]AGC
2226 GCTCAGC
Bpu1102I 1557 GCTCAGC GC^T[ACGT]AGC
2226 GCTCAGC
BspHI 1660 TCATGA T^CATGA
2125 TCATGA
Bsu36I 1922 CCTGAGG CC^T[ACGT]AGG
2614 CCTGAGG
CelII 1557 GCTCAGC GC^T[ACGT]AGC
2226 GCTCAGC
EarI 1266 CTCTTC CTCTTC(1/4)
1824 CTCTTC
Eco81I 1922 CCTGAGG CC^T[ACGT]AGG
2614 CCTGAGG
EcoNI 890 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1918 CCTTCCTGAGG
FspI 742 TGCGCA TGC^GCA
2248 TGCGCA
Ksp632I 1266 CTCTTC CTCTTC(1/4)
1824 CTCTTC
MaeI 462 CTAG C^TAG
720 CTAG
NcoI 61 CCATGG C^CATGG
1247 CCATGG
PshAI 482 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2303 GACCACCGTC
PvuI 377 CGATCG CGAT^CG
2635 CGATCG
RcaI 1660 TCATGA T^CATGA
2125 TCATGA
XcmI 592 CCAACTCGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2044 CCATCCGGAACCTGG
XmnI 326 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1790 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII