##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Aor51HI AscI
Asp718I AspEI AvaI AviII BalI
BbeI BbrPI BbvCI BcnI BglII
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
FseI FspI HgaI Hin1I KasI
KpnI KspI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AciI 2465 GCGG CCGC(-3/-1)
AflIII 678 ACATGT A^C[GA][CT]GT
Alw44I 1426 GTGCAC G^TGCAC
AocI 255 CCTCAGG CC^T[ACGT]AGG
ApaI 322 GGGCCC GGGCC^C
ApaLI 1426 GTGCAC G^TGCAC
Asp700I 1797 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1174 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1999 CCTAGG C^CTAGG
BcgI 3096 GCAGTTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3577 GTATCC GTATCC(6/5)
BclI 4917 TGATCA T^GATCA
BglI 3327 GCCGACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1999 CCTAGG C^CTAGG
BsaBI 4671 GATAGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2264 GGTCTC GGTCTC(1/5)
BsaWI 4778 ACCGGA [AT]^CCGG[AT]
Bsp1407I 4717 TGTACA T^GTACA
BspLU11I 678 ACATGT A^CATGT
BsrFI 211 ACCGGC [GA]^CCGG[CT]
BsrGI 4717 TGTACA T^GTACA
BstXI 2472 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 255 CCTCAGG CC^T[ACGT]AGG
Cfr10I 211 ACCGGC [GA]^CCGG[CT]
DraIII 2895 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 294 GACAATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 255 CCTCAGG CC^T[ACGT]AGG
EcoRV 974 GATATC GAT^ATC
FauI 2465 GCGGG CCCGC(4/6)
FbaI 4917 TGATCA T^GATCA
HaeII 4047 GGCGCT [GA]GCGC^[CT]
HpaI 3815 GTTAAC GTT^AAC
MaeII 1161 ACGT A^CGT
MamI 4671 GATAGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 136 CAATTG C^AATTG
MunI 136 CAATTG C^AATTG
PflFI 1174 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 164 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 877 GAAGAGC GCTCTTC(1/4)
SpeI 2072 ACTAGT A^CTAGT
SphI 3301 GCATGC GCATG^C
SspBI 4717 TGTACA T^GTACA
Tth111I 1174 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1144 TCTAGA T^CTAGA
XmnI 1797 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 3071 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
3120 CAGCTGCTG
BamHI 4676 GGATCC G^GATCC
4691 GGATCC
BanII 322 GGGCCC G[GA]GC[CT]^C
4814 GAGCCC
BlpI 1048 GCTTAGC GC^T[ACGT]AGC
1752 GCTCAGC
Bpu1102I 1048 GCTTAGC GC^T[ACGT]AGC
1752 GCTCAGC
BsmI 4272 GAATGC GAATGC(1/-1)
1614 GCATTC
BspMI 3201 ACCTGC ACCTGC(4/8)
3987 GCAGGT
CelII 1048 GCTTAGC GC^T[ACGT]AGC
1752 GCTCAGC
CfoI 1022 GCGC GCG^C
4048 GCGC
EcoT22I 124 ATGCAT ATGCA^T
174 ATGCAT
HapII 212 CCGG C^CGG
4779 CCGG
HhaI 1022 GCGC GCG^C
4048 GCGC
HinP1I 1022 GCGC G^CGC
4048 GCGC
HincII 1180 GTCAAC GT[CT]^[GA]AC
3815 GTTAAC
HindII 1180 GTCAAC GT[CT]^[GA]AC
3815 GTTAAC
HindIII 3728 AAGCTT A^AGCTT
3917 AAGCTT
HpaII 212 CCGG C^CGG
4779 CCGG
MspI 212 CCGG C^CGG
4779 CCGG
NsiI 124 ATGCAT ATGCA^T
174 ATGCAT
PacI 3779 TTAATTAA TTAAT^TAA
4382 TTAATTAA
PflMI 1442 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2655 CCAGGATGTGG
PpuMI 867 AGGACCT [GA]G^G[AT]CC[CT]
3698 AGGTCCC
SspI 1941 AATATT AAT^ATT
3906 AATATT
StuI 4009 AGGCCT AGG^CCT
4500 AGGCCT
TaqI 506 TCGA T^CGA
3797 TCGA
TthHB8I 506 TCGA T^CGA
3797 TCGA
Van91I 1442 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2655 CCAGGATGTGG
XcmI 841 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4888 CCATATTTACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AcsI AfaI AflII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanI BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII