################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AviII BbeI BciVI BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MroI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 21 GACGTC GACGT^C AccI 3885 GTCTAC GT^[AC][GT]AC AcsI 5046 GAATTC [GA]^AATT[CT] AcyI 21 GACGTC G[GA]^CG[CT]C AflII 1703 CTTAAG C^TTAAG AflIII 4807 ACGCGT A^C[GA][CT]GT AhdI 582 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1125 GTGCAC G^TGCAC ApaLI 1125 GTGCAC G^TGCAC ApoI 5046 GAATTC [GA]^AATT[CT] AspEI 582 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 740 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 859 CCTAGG C^CTAGG BamHI 3070 GGATCC G^GATCC BclI 3646 TGATCA T^GATCA BfrI 1703 CTTAAG C^TTAAG BlnI 859 CCTAGG C^CTAGG BsaHI 21 GACGTC G[GA]^CG[CT]C BsmBI 2159 GAGACG CGTCTC(1/5) BspHI 2676 TCATGA T^CATGA BsrBI 4207 CCGCTC CCGCTC(-3/-3) BsrDI 3770 GCAATG GCAATG(2/0) BssSI 1758 CACGAG CACGAG(-5/-1) BstXI 789 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 2743 GACCATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 582 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 5046 GAATTC G^AATTC EcoRV 3367 GATATC GAT^ATC FbaI 3646 TGATCA T^GATCA HaeII 1866 GGCGCT [GA]GCGC^[CT] Hin1I 21 GACGTC G[GA]^CG[CT]C HincII 4390 GTTGAC GT[CT]^[GA]AC HindII 4390 GTTGAC GT[CT]^[GA]AC MfeI 4189 CAATTG C^AATTG MluI 4807 ACGCGT A^CGCGT MunI 4189 CAATTG C^AATTG NruI 4905 TCGCGA TCG^CGA PaeR7I 1375 CTCGAG C^TCGAG PflFI 740 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 2676 TCATGA T^CATGA SapI 270 GAAGAGC GCTCTTC(1/4) SexAI 3107 ACCAGGT A^CC[AT]GGT SphI 1778 GCATGC GCATG^C Tth111I 740 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4655 TCTAGA T^CTAGA XhoI 1375 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AocI 635 CCTGAGG CC^T[ACGT]AGG 1922 CCTGAGG BbrPI 212 CACGTG CAC^GTG 675 CACGTG BbvCI 3429 CCTCAGC CCTCAGC(-5/-2) 2138 GCTGAGG BcgI 77 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2994 CGAGTGACCTGC BglI 515 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1893 GCCTGCCTGGC BglII 774 AGATCT A^GATCT 4312 AGATCT BsaI 1796 GAGACC GGTCTC(1/5) 1981 GAGACC BsaWI 1260 TCCGGT [AT]^CCGG[AT] 1270 TCCGGT BstAPI 311 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1357 GCAGCCTTTGC Bsu36I 635 CCTGAGG CC^T[ACGT]AGG 1922 CCTGAGG DraIII 2184 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG 2984 CACTGGGTG Eco81I 635 CCTGAGG CC^T[ACGT]AGG 1922 CCTGAGG EcoNI 2061 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3160 CCTTGTAAAGG HgaI 1495 GACGC GACGC(5/10) 1679 GCGTC PflMI 1601 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3061 CCATGCTTTGG PmaCI 212 CACGTG CAC^GTG 675 CACGTG PmlI 212 CACGTG CAC^GTG 675 CACGTG PshAI 293 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3957 GACTCAAGTC Van91I 1601 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3061 CCATGCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI