##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AviII BbeI BciVI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoT22I FseI FspI HpaI KasI
KpnI KspI MamI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 21 GACGTC GACGT^C
AccI 3885 GTCTAC GT^[AC][GT]AC
AcsI 5046 GAATTC [GA]^AATT[CT]
AcyI 21 GACGTC G[GA]^CG[CT]C
AflII 1703 CTTAAG C^TTAAG
AflIII 4807 ACGCGT A^C[GA][CT]GT
AhdI 582 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1125 GTGCAC G^TGCAC
ApaLI 1125 GTGCAC G^TGCAC
ApoI 5046 GAATTC [GA]^AATT[CT]
AspEI 582 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 740 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 859 CCTAGG C^CTAGG
BamHI 3070 GGATCC G^GATCC
BclI 3646 TGATCA T^GATCA
BfrI 1703 CTTAAG C^TTAAG
BlnI 859 CCTAGG C^CTAGG
BsaHI 21 GACGTC G[GA]^CG[CT]C
BsmBI 2159 GAGACG CGTCTC(1/5)
BspHI 2676 TCATGA T^CATGA
BsrBI 4207 CCGCTC CCGCTC(-3/-3)
BsrDI 3770 GCAATG GCAATG(2/0)
BssSI 1758 CACGAG CACGAG(-5/-1)
BstXI 789 CCAGGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2743 GACCATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 582 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5046 GAATTC G^AATTC
EcoRV 3367 GATATC GAT^ATC
FbaI 3646 TGATCA T^GATCA
HaeII 1866 GGCGCT [GA]GCGC^[CT]
Hin1I 21 GACGTC G[GA]^CG[CT]C
HincII 4390 GTTGAC GT[CT]^[GA]AC
HindII 4390 GTTGAC GT[CT]^[GA]AC
MfeI 4189 CAATTG C^AATTG
MluI 4807 ACGCGT A^CGCGT
MunI 4189 CAATTG C^AATTG
NruI 4905 TCGCGA TCG^CGA
PaeR7I 1375 CTCGAG C^TCGAG
PflFI 740 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2676 TCATGA T^CATGA
SapI 270 GAAGAGC GCTCTTC(1/4)
SexAI 3107 ACCAGGT A^CC[AT]GGT
SphI 1778 GCATGC GCATG^C
Tth111I 740 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4655 TCTAGA T^CTAGA
XhoI 1375 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 635 CCTGAGG CC^T[ACGT]AGG
1922 CCTGAGG
BbrPI 212 CACGTG CAC^GTG
675 CACGTG
BbvCI 3429 CCTCAGC CCTCAGC(-5/-2)
2138 GCTGAGG
BcgI 77 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2994 CGAGTGACCTGC
BglI 515 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1893 GCCTGCCTGGC
BglII 774 AGATCT A^GATCT
4312 AGATCT
BsaI 1796 GAGACC GGTCTC(1/5)
1981 GAGACC
BsaWI 1260 TCCGGT [AT]^CCGG[AT]
1270 TCCGGT
BstAPI 311 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1357 GCAGCCTTTGC
Bsu36I 635 CCTGAGG CC^T[ACGT]AGG
1922 CCTGAGG
DraIII 2184 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2984 CACTGGGTG
Eco81I 635 CCTGAGG CC^T[ACGT]AGG
1922 CCTGAGG
EcoNI 2061 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3160 CCTTGTAAAGG
HgaI 1495 GACGC GACGC(5/10)
1679 GCGTC
PflMI 1601 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3061 CCATGCTTTGG
PmaCI 212 CACGTG CAC^GTG
675 CACGTG
PmlI 212 CACGTG CAC^GTG
675 CACGTG
PshAI 293 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3957 GACTCAAGTC
Van91I 1601 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3061 CCATGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI