##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp718I AspEI AspI AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BfrI BlnI BsiWI BsmI
BspDI BspLU11I BsrBI BstBI BstEII
BstPI Bsu36I ClaI CpoI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI KasI KpnI KspI
MluNI MscI NarI NcoI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 2293 TCCGGA T^CCGGA
AcyI 2843 GACGCC G[GA]^CG[CT]C
AflIII 363 ACGCGT A^C[GA][CT]GT
AseI 1385 ATTAAT AT^TAAT
AsnI 1385 ATTAAT AT^TAAT
BanI 1037 GGCACC G^G[CT][GA]CC
BciVI 815 GTATCC GTATCC(6/5)
BlpI 655 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 655 GCTCAGC GC^T[ACGT]AGC
BsaAI 71 CACGTA [CT]AC^GT[GA]
BsaHI 2843 GACGCC G[GA]^CG[CT]C
BseAI 2293 TCCGGA T^CCGGA
BsmBI 185 CGTCTC CGTCTC(1/5)
BspEI 2293 TCCGGA T^CCGGA
BssSI 1661 CACGAG CACGAG(-5/-1)
Bst1107I 3125 GTATAC GTA^TAC
BstZ17I 3125 GTATAC GTA^TAC
CelII 655 GCTCAGC GC^T[ACGT]AGC
Hin1I 2843 GACGCC G[GA]^CG[CT]C
HpaI 3401 GTTAAC GTT^AAC
MfeI 3064 CAATTG C^AATTG
MluI 363 ACGCGT A^CGCGT
MroI 2293 TCCGGA T^CCGGA
MunI 3064 CAATTG C^AATTG
NruI 1906 TCGCGA TCG^CGA
PmeI 3472 GTTTAAAC GTTT^AAAC
PshAI 712 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1385 ATTAAT AT^TAAT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 323 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
658 CAGCCGCTG
Aor51HI 276 AGCGCT AGC^GCT
481 AGCGCT
Asp700I 619 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1516 GAACGACTTC
AviII 2046 TGCGCA TGC^GCA
2644 TGCGCA
BamHI 791 GGATCC G^GATCC
1356 GGATCC
BbsI 1616 GAAGAC GAAGAC(2/6)
1087 GTCTTC
BcnI 238 CCGGG CC^[GC]GG
3248 CCCGG
BglI 306 GCCACGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
393 GCCAGGAGGGC
BpuAI 1616 GAAGAC GAAGAC(2/6)
1087 GTCTTC
BsaWI 1278 ACCGGA [AT]^CCGG[AT]
2293 TCCGGA
Bsp1407I 3171 TGTACA T^GTACA
3884 TGTACA
BsrGI 3171 TGTACA T^GTACA
3884 TGTACA
DraI 3422 TTTAAA TTT^AAA
3473 TTTAAA
DraIII 58 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1267 CACTGTGTG
DrdI 1078 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1670 GACAGTGGTGTC
EagI 235 CGGCCG C^GGCCG
763 CGGCCG
EclXI 235 CGGCCG C^GGCCG
763 CGGCCG
Eco47III 276 AGCGCT AGC^GCT
481 AGCGCT
Eco52I 235 CGGCCG C^GGCCG
763 CGGCCG
EcoRV 1181 GATATC GAT^ATC
3746 GATATC
FspI 2046 TGCGCA TGC^GCA
2644 TGCGCA
HindIII 933 AAGCTT A^AGCTT
2355 AAGCTT
NaeI 83 GCCGGC GCC^GGC
539 GCCGGC
NciI 238 CCGGG CC^[GC]GG
3248 CCCGG
NgoMIV 83 GCCGGC G^CCGGC
539 GCCGGC
SapI 1130 GAAGAGC GCTCTTC(1/4)
1205 GAAGAGC
SspBI 3171 TGTACA T^GTACA
3884 TGTACA
SspI 3352 AATATT AAT^ATT
3855 AATATT
XmnI 619 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1516 GAACGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbvI BfaI BglII
BmyI BpmI BsaBI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII