################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AspI AvrII BalI BbeI BbrPI BbvCI BcgI BclI BfrI BlnI BsiWI BsmI BspDI BspLU11I BsrBI BstBI BstEII BstPI Bsu36I ClaI CpoI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI KasI KpnI KspI MluNI MscI NarI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccIII 2293 TCCGGA T^CCGGA AcyI 2843 GACGCC G[GA]^CG[CT]C AflIII 363 ACGCGT A^C[GA][CT]GT AseI 1385 ATTAAT AT^TAAT AsnI 1385 ATTAAT AT^TAAT BanI 1037 GGCACC G^G[CT][GA]CC BciVI 815 GTATCC GTATCC(6/5) BlpI 655 GCTCAGC GC^T[ACGT]AGC Bpu1102I 655 GCTCAGC GC^T[ACGT]AGC BsaAI 71 CACGTA [CT]AC^GT[GA] BsaHI 2843 GACGCC G[GA]^CG[CT]C BseAI 2293 TCCGGA T^CCGGA BsmBI 185 CGTCTC CGTCTC(1/5) BspEI 2293 TCCGGA T^CCGGA BssSI 1661 CACGAG CACGAG(-5/-1) Bst1107I 3125 GTATAC GTA^TAC BstZ17I 3125 GTATAC GTA^TAC CelII 655 GCTCAGC GC^T[ACGT]AGC Hin1I 2843 GACGCC G[GA]^CG[CT]C HpaI 3401 GTTAAC GTT^AAC MfeI 3064 CAATTG C^AATTG MluI 363 ACGCGT A^CGCGT MroI 2293 TCCGGA T^CCGGA MunI 3064 CAATTG C^AATTG NruI 1906 TCGCGA TCG^CGA PmeI 3472 GTTTAAAC GTTT^AAAC PshAI 712 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1385 ATTAAT AT^TAAT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 323 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 658 CAGCCGCTG Aor51HI 276 AGCGCT AGC^GCT 481 AGCGCT Asp700I 619 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1516 GAACGACTTC AviII 2046 TGCGCA TGC^GCA 2644 TGCGCA BamHI 791 GGATCC G^GATCC 1356 GGATCC BbsI 1616 GAAGAC GAAGAC(2/6) 1087 GTCTTC BcnI 238 CCGGG CC^[GC]GG 3248 CCCGG BglI 306 GCCACGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 393 GCCAGGAGGGC BpuAI 1616 GAAGAC GAAGAC(2/6) 1087 GTCTTC BsaWI 1278 ACCGGA [AT]^CCGG[AT] 2293 TCCGGA Bsp1407I 3171 TGTACA T^GTACA 3884 TGTACA BsrGI 3171 TGTACA T^GTACA 3884 TGTACA DraI 3422 TTTAAA TTT^AAA 3473 TTTAAA DraIII 58 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 1267 CACTGTGTG DrdI 1078 GACAAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1670 GACAGTGGTGTC EagI 235 CGGCCG C^GGCCG 763 CGGCCG EclXI 235 CGGCCG C^GGCCG 763 CGGCCG Eco47III 276 AGCGCT AGC^GCT 481 AGCGCT Eco52I 235 CGGCCG C^GGCCG 763 CGGCCG EcoRV 1181 GATATC GAT^ATC 3746 GATATC FspI 2046 TGCGCA TGC^GCA 2644 TGCGCA HindIII 933 AAGCTT A^AGCTT 2355 AAGCTT NaeI 83 GCCGGC GCC^GGC 539 GCCGGC NciI 238 CCGGG CC^[GC]GG 3248 CCCGG NgoMIV 83 GCCGGC G^CCGGC 539 GCCGGC SapI 1130 GAAGAGC GCTCTTC(1/4) 1205 GAAGAGC SspBI 3171 TGTACA T^GTACA 3884 TGTACA SspI 3352 AATATT AAT^ATT 3855 AATATT XmnI 619 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1516 GAACGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanII BbvI BfaI BglII BmyI BpmI BsaBI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII