##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        Asp718I     AspEI       AspI        AvrII       
BalI        BbeI        BbrPI       BbvCI       BcgI        
BclI        BfrI        BlnI        BsiWI       BsmI        
BspDI       BspLU11I    BsrBI       BstBI       BstEII      
BstPI       Bsu36I      ClaI        CpoI        Eam1105I    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FbaI        FseI        KasI        KpnI        KspI        
MluNI       MscI        NarI        NcoI        NdeI        
NheI        NotI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmlI        
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AccIII       2293  TCCGGA               T^CCGGA
AcyI         2843  GACGCC               G[GA]^CG[CT]C
AflIII        363  ACGCGT               A^C[GA][CT]GT
AseI         1385  ATTAAT               AT^TAAT
AsnI         1385  ATTAAT               AT^TAAT
BanI         1037  GGCACC               G^G[CT][GA]CC
BciVI         815  GTATCC               GTATCC(6/5)
BlpI          655  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      655  GCTCAGC              GC^T[ACGT]AGC
BsaAI          71  CACGTA               [CT]AC^GT[GA]
BsaHI        2843  GACGCC               G[GA]^CG[CT]C
BseAI        2293  TCCGGA               T^CCGGA
BsmBI         185  CGTCTC               CGTCTC(1/5)
BspEI        2293  TCCGGA               T^CCGGA
BssSI        1661  CACGAG               CACGAG(-5/-1)
Bst1107I     3125  GTATAC               GTA^TAC
BstZ17I      3125  GTATAC               GTA^TAC
CelII         655  GCTCAGC              GC^T[ACGT]AGC
Hin1I        2843  GACGCC               G[GA]^CG[CT]C
HpaI         3401  GTTAAC               GTT^AAC
MfeI         3064  CAATTG               C^AATTG
MluI          363  ACGCGT               A^CGCGT
MroI         2293  TCCGGA               T^CCGGA
MunI         3064  CAATTG               C^AATTG
NruI         1906  TCGCGA               TCG^CGA
PmeI         3472  GTTTAAAC             GTTT^AAAC
PshAI         712  GACTCTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1385  ATTAAT               AT^TAAT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AlwNI         323  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              658  CAGCCGCTG           
Aor51HI       276  AGCGCT               AGC^GCT
              481  AGCGCT              
Asp700I       619  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1516  GAACGACTTC          
AviII        2046  TGCGCA               TGC^GCA
             2644  TGCGCA              
BamHI         791  GGATCC               G^GATCC
             1356  GGATCC              
BbsI         1616  GAAGAC               GAAGAC(2/6)
             1087  GTCTTC              
BcnI          238  CCGGG                CC^[GC]GG
             3248  CCCGG               
BglI          306  GCCACGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              393  GCCAGGAGGGC         
BpuAI        1616  GAAGAC               GAAGAC(2/6)
             1087  GTCTTC              
BsaWI        1278  ACCGGA               [AT]^CCGG[AT]
             2293  TCCGGA              
Bsp1407I     3171  TGTACA               T^GTACA
             3884  TGTACA              
BsrGI        3171  TGTACA               T^GTACA
             3884  TGTACA              
DraI         3422  TTTAAA               TTT^AAA
             3473  TTTAAA              
DraIII         58  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1267  CACTGTGTG           
DrdI         1078  GACAAAGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1670  GACAGTGGTGTC        
EagI          235  CGGCCG               C^GGCCG
              763  CGGCCG              
EclXI         235  CGGCCG               C^GGCCG
              763  CGGCCG              
Eco47III      276  AGCGCT               AGC^GCT
              481  AGCGCT              
Eco52I        235  CGGCCG               C^GGCCG
              763  CGGCCG              
EcoRV        1181  GATATC               GAT^ATC
             3746  GATATC              
FspI         2046  TGCGCA               TGC^GCA
             2644  TGCGCA              
HindIII       933  AAGCTT               A^AGCTT
             2355  AAGCTT              
NaeI           83  GCCGGC               GCC^GGC
              539  GCCGGC              
NciI          238  CCGGG                CC^[GC]GG
             3248  CCCGG               
NgoMIV         83  GCCGGC               G^CCGGC
              539  GCCGGC              
SapI         1130  GAAGAGC              GCTCTTC(1/4)
             1205  GAAGAGC             
SspBI        3171  TGTACA               T^GTACA
             3884  TGTACA              
SspI         3352  AATATT               AAT^ATT
             3855  AATATT              
XmnI          619  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1516  GAACGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        ApoI        AspHI       AvaI        
AvaII       BanII       BbvI        BfaI        BglII       
BmyI        BpmI        BsaBI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrDI       BsrFI       BsrI        
BssHII      BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RcaI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII