################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AhdI AocI AscI AseI AsnI Asp718I AspEI AspI BbeI BfrI BlpI Bpu1102I BsaHI BseAI BsiEI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I FseI Hin1I KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SfoI SmlI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AgeI 1310 ACCGGT A^CCGGT Aor51HI 853 AGCGCT AGC^GCT ApaI 4099 GGGCCC GGGCC^C AviII 1126 TGCGCA TGC^GCA AvrII 4222 CCTAGG C^CTAGG BamHI 2996 GGATCC G^GATCC BcgI 1016 CGACCAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3709 GGATAC GTATCC(6/5) BclI 2061 TGATCA T^GATCA BglII 47 AGATCT A^GATCT BlnI 4222 CCTAGG C^CTAGG BsaBI 1049 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 3483 CGTACG C^GTACG BspLU11I 1141 ACATGT A^CATGT BssHII 124 GCGCGC G^CGCGC CpoI 4200 CGGACCG CG^G[AT]CCG DraI 2672 TTTAAA TTT^AAA DraIII 1195 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 853 AGCGCT AGC^GCT EcoRI 3530 GAATTC G^AATTC EcoRV 1319 GATATC GAT^ATC FbaI 2061 TGATCA T^GATCA FspI 1126 TGCGCA TGC^GCA HpaI 2447 GTTAAC GTT^AAC MamI 1049 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1118 CAATTG C^AATTG MunI 1118 CAATTG C^AATTG NheI 1486 GCTAGC G^CTAGC PinAI 1310 ACCGGT A^CCGGT PshAI 1790 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 4200 CGGACCG CG^G[AT]CCG SapI 2911 GCTCTTC GCTCTTC(1/4) SfiI 1471 GGCCACGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1309 CACCGGTG C[GA]^CCGG[CT]G SpeI 2712 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Alw44I 1340 GTGCAC G^TGCAC 1457 GTGCAC AlwNI 255 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG 4107 CAGTCCCTG ApaLI 1340 GTGCAC G^TGCAC 1457 GTGCAC Asp700I 1762 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1897 GAAGGACTTC BbrPI 3425 CACGTG CAC^GTG 4121 CACGTG BbvCI 4213 CCTCAGC CCTCAGC(-5/-2) 1586 GCTGAGG BglI 798 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1472 GCCACGCTGGC BsaAI 3425 CACGTG [CT]AC^GT[GA] 4121 CACGTG BsaI 1530 GAGACC GGTCTC(1/5) 288 GGTCTC BsrFI 1310 ACCGGT [GA]^CCGG[CT] 3453 ACCGGC BssSI 3557 CACGAG CACGAG(-5/-1) 1167 CTCGTG BstBI 3098 TTCGAA TT^CGAA 4244 TTCGAA Cfr10I 1310 ACCGGT [GA]^CCGG[CT] 3453 ACCGGC EcoT22I 1040 ATGCAT ATGCA^T 3044 ATGCAT HaeII 351 AGCGCC [GA]GCGC^[CT] 853 AGCGCT HincII 2447 GTTAAC GT[CT]^[GA]AC 2704 GTTGAC HindII 2447 GTTAAC GT[CT]^[GA]AC 2704 GTTGAC HindIII 185 AAGCTT A^AGCTT 1270 AAGCTT NcoI 7 CCATGG C^CATGG 3871 CCATGG NdeI 293 CATATG CA^TATG 1427 CATATG NsiI 1040 ATGCAT ATGCA^T 3044 ATGCAT NspV 3098 TTCGAA TT^CGAA 4244 TTCGAA PflMI 1197 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2029 CCAAATGCTGG PmaCI 3425 CACGTG CAC^GTG 4121 CACGTG PmlI 3425 CACGTG CAC^GTG 4121 CACGTG PvuII 2359 CAGCTG CAG^CTG 2849 CAGCTG SfuI 3098 TTCGAA TT^CGAA 4244 TTCGAA SmaI 247 CCCGGG CCC^GGG 1806 CCCGGG SphI 511 GCATGC GCATG^C 1042 GCATGC Van91I 1197 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2029 CCAAATGCTGG XmaCI 247 CCCGGG C^CCGGG 1806 CCCGGG XmaI 247 CCCGGG C^CCGGG 1806 CCCGGG XmnI 1762 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1897 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII