##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AhdI AocI AscI
AseI AsnI Asp718I AspEI AspI
BbeI BfrI BlpI Bpu1102I BsaHI
BseAI BsiEI BsmBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
ClaI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I FseI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NotI NruI
PacI PaeR7I PflFI PmeI PshBI
Psp1406I PvuI RcaI SacII SalI
SbfI ScaI SfoI SmlI SnaBI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AgeI 1310 ACCGGT A^CCGGT
Aor51HI 853 AGCGCT AGC^GCT
ApaI 4099 GGGCCC GGGCC^C
AviII 1126 TGCGCA TGC^GCA
AvrII 4222 CCTAGG C^CTAGG
BamHI 2996 GGATCC G^GATCC
BcgI 1016 CGACCAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3709 GGATAC GTATCC(6/5)
BclI 2061 TGATCA T^GATCA
BglII 47 AGATCT A^GATCT
BlnI 4222 CCTAGG C^CTAGG
BsaBI 1049 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 3483 CGTACG C^GTACG
BspLU11I 1141 ACATGT A^CATGT
BssHII 124 GCGCGC G^CGCGC
CpoI 4200 CGGACCG CG^G[AT]CCG
DraI 2672 TTTAAA TTT^AAA
DraIII 1195 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 853 AGCGCT AGC^GCT
EcoRI 3530 GAATTC G^AATTC
EcoRV 1319 GATATC GAT^ATC
FbaI 2061 TGATCA T^GATCA
FspI 1126 TGCGCA TGC^GCA
HpaI 2447 GTTAAC GTT^AAC
MamI 1049 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1118 CAATTG C^AATTG
MunI 1118 CAATTG C^AATTG
NheI 1486 GCTAGC G^CTAGC
PinAI 1310 ACCGGT A^CCGGT
PshAI 1790 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4200 CGGACCG CG^G[AT]CCG
SapI 2911 GCTCTTC GCTCTTC(1/4)
SfiI 1471 GGCCACGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1309 CACCGGTG C[GA]^CCGG[CT]G
SpeI 2712 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 1340 GTGCAC G^TGCAC
1457 GTGCAC
AlwNI 255 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
4107 CAGTCCCTG
ApaLI 1340 GTGCAC G^TGCAC
1457 GTGCAC
Asp700I 1762 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1897 GAAGGACTTC
BbrPI 3425 CACGTG CAC^GTG
4121 CACGTG
BbvCI 4213 CCTCAGC CCTCAGC(-5/-2)
1586 GCTGAGG
BglI 798 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1472 GCCACGCTGGC
BsaAI 3425 CACGTG [CT]AC^GT[GA]
4121 CACGTG
BsaI 1530 GAGACC GGTCTC(1/5)
288 GGTCTC
BsrFI 1310 ACCGGT [GA]^CCGG[CT]
3453 ACCGGC
BssSI 3557 CACGAG CACGAG(-5/-1)
1167 CTCGTG
BstBI 3098 TTCGAA TT^CGAA
4244 TTCGAA
Cfr10I 1310 ACCGGT [GA]^CCGG[CT]
3453 ACCGGC
EcoT22I 1040 ATGCAT ATGCA^T
3044 ATGCAT
HaeII 351 AGCGCC [GA]GCGC^[CT]
853 AGCGCT
HincII 2447 GTTAAC GT[CT]^[GA]AC
2704 GTTGAC
HindII 2447 GTTAAC GT[CT]^[GA]AC
2704 GTTGAC
HindIII 185 AAGCTT A^AGCTT
1270 AAGCTT
NcoI 7 CCATGG C^CATGG
3871 CCATGG
NdeI 293 CATATG CA^TATG
1427 CATATG
NsiI 1040 ATGCAT ATGCA^T
3044 ATGCAT
NspV 3098 TTCGAA TT^CGAA
4244 TTCGAA
PflMI 1197 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2029 CCAAATGCTGG
PmaCI 3425 CACGTG CAC^GTG
4121 CACGTG
PmlI 3425 CACGTG CAC^GTG
4121 CACGTG
PvuII 2359 CAGCTG CAG^CTG
2849 CAGCTG
SfuI 3098 TTCGAA TT^CGAA
4244 TTCGAA
SmaI 247 CCCGGG CCC^GGG
1806 CCCGGG
SphI 511 GCATGC GCATG^C
1042 GCATGC
Van91I 1197 CCAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2029 CCAAATGCTGG
XmaCI 247 CCCGGG C^CCGGG
1806 CCCGGG
XmaI 247 CCCGGG C^CCGGG
1806 CCCGGG
XmnI 1762 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1897 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII