##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BalI
BbrPI BbvCI BcgI BcnI BfrI
BglI BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FauI
FseI FspI HapII HpaI HpaII
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NciI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 419 GACGTC GACGT^C
AclI 512 AACGTT AA^CGTT
Alw44I 4091 GTGCAC G^TGCAC
AlwNI 286 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1401 CCTGAGG CC^T[ACGT]AGG
ApaLI 4091 GTGCAC G^TGCAC
AvaI 407 CTCGAG C^[CT]CG[GA]G
BanII 969 GAGCCC G[GA]GC[CT]^C
BbeI 3385 GGCGCC GGCGC^C
BclI 2049 TGATCA T^GATCA
BlpI 2079 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2079 GCTGAGC GC^T[ACGT]AGC
BsgI 442 GTGCAG GTGCAG(16/14)
BsmI 577 GAATGC GAATGC(1/-1)
BsoBI 407 CTCGAG C^[CT]CG[GA]G
BsrDI 462 CATTGC GCAATG(2/0)
Bst1107I 2549 GTATAC GTA^TAC
BstAPI 3942 GCAAACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2549 GTATAC GTA^TAC
Bsu36I 1401 CCTGAGG CC^T[ACGT]AGG
CelII 2079 GCTGAGC GC^T[ACGT]AGC
DrdI 1800 GACATACTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 29 CCATGG C^C[GA][CT]GG
Eco81I 1401 CCTGAGG CC^T[ACGT]AGG
EcoRI 118 GAATTC G^AATTC
EcoT22I 4357 ATGCAT ATGCA^T
FbaI 2049 TGATCA T^GATCA
HgaI 14 GCGTC GACGC(5/10)
HindIII 1537 AAGCTT A^AGCTT
KasI 3385 GGCGCC G^GCGCC
NarI 3385 GGCGCC GG^CGCC
NcoI 29 CCATGG C^CATGG
NheI 909 GCTAGC G^CTAGC
NsiI 4357 ATGCAT ATGCA^T
PaeR7I 407 CTCGAG C^TCGAG
Psp1406I 512 AACGTT AA^CGTT
PvuII 3069 CAGCTG CAG^CTG
SalI 159 GTCGAC G^TCGAC
SfoI 3385 GGCGCC GGC^GCC
StuI 2097 AGGCCT AGG^CCT
XbaI 2834 TCTAGA T^CTAGA
XhoI 407 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 419 GACGTC G[GA]^CG[CT]C
3385 GGCGCC
AspHI 2414 GTGCTC G[AT]GC[AT]^C
4091 GTGCAC
BamHI 2948 GGATCC G^GATCC
3621 GGATCC
BanI 25 GGCACC G^G[CT][GA]CC
3385 GGCGCC
BciVI 207 GTATCC GTATCC(6/5)
768 GTATCC
BglII 2793 AGATCT A^GATCT
3547 AGATCT
BsaHI 419 GACGTC G[GA]^CG[CT]C
3385 GGCGCC
BsiHKAI 2414 GTGCTC G[AT]GC[AT]^C
4091 GTGCAC
BsmFI 1406 GGGAC GGGAC(10/14)
2772 GGGAC
BspHI 1687 TCATGA T^CATGA
2442 TCATGA
BstXI 2382 CCAACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3653 CCATAATGATGG
CfoI 3302 GCGC GCG^C
3386 GCGC
HaeII 3301 GGCGCT [GA]GCGC^[CT]
3385 GGCGCC
HhaI 3302 GCGC GCG^C
3386 GCGC
Hin1I 419 GACGTC G[GA]^CG[CT]C
3385 GGCGCC
HinP1I 3302 GCGC G^CGC
3386 GCGC
HincII 16 GTCAAC GT[CT]^[GA]AC
159 GTCGAC
HindII 16 GTCAAC GT[CT]^[GA]AC
159 GTCGAC
MspA1I 3069 CAGCTG C[AC]G^C[GT]G
3381 CAGCGG
RcaI 1687 TCATGA T^CATGA
2442 TCATGA
SmlI 49 CTTGAG C^T[CT][GA]AG
407 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I Bsp1407I
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SpeI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI