##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AclI AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AvaI
BciVI BclI BlpI Bpu1102I BsiEI
BsiWI BsoBI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV FbaI FseI HpaI
KspI MluI NheI NotI NruI
NspV PacI PaeR7I PinAI Psp1406I
PvuI RsrII SacI SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SmaI SpeI SspBI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 2271 GGTACC G^GTACC
AcyI 1218 GGCGCC G[GA]^CG[CT]C
AflII 2539 CTTAAG C^TTAAG
AhdI 4234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4792 ATTAAT AT^TAAT
AsnI 4792 ATTAAT AT^TAAT
Asp718I 2271 GGTACC G^GTACC
AspEI 4234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 227 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3743 TGCGCA TGC^GCA
BamHI 4663 GGATCC G^GATCC
BbeI 1218 GGCGCC GGCGC^C
BbvCI 1775 GCTGAGG CCTCAGC(-5/-2)
BcgI 1398 GCAACATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2539 CTTAAG C^TTAAG
BglI 1082 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 2374 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1218 GGCGCC G[GA]^CG[CT]C
BsgI 1421 CTGCAC GTGCAG(16/14)
BsmBI 3374 CGTCTC CGTCTC(1/5)
BspHI 622 TCATGA T^CATGA
BspMI 1520 ACCTGC ACCTGC(4/8)
BsrDI 1914 CATTGC GCAATG(2/0)
BssSI 41 CACGAG CACGAG(-5/-1)
BstEII 4251 GGTTACC G^GT[ACGT]ACC
BstPI 4251 GGTTACC G^GT[ACGT]ACC
DrdI 3309 GACAAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4234 GACTGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4251 GGTTACC G^GT[ACGT]ACC
EcoT22I 188 ATGCAT ATGCA^T
FspI 3743 TGCGCA TGC^GCA
HaeII 1218 GGCGCC [GA]GCGC^[CT]
Hin1I 1218 GGCGCC G[GA]^CG[CT]C
HincII 4499 GTTGAC GT[CT]^[GA]AC
HindII 4499 GTTGAC GT[CT]^[GA]AC
KasI 1218 GGCGCC G^GCGCC
KpnI 2271 GGTACC GGTAC^C
MamI 2374 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2019 CAATTG C^AATTG
MunI 2019 CAATTG C^AATTG
NaeI 2176 GCCGGC GCC^GGC
NarI 1218 GGCGCC GG^CGCC
NgoMIV 2176 GCCGGC G^CCGGC
NsiI 188 ATGCAT ATGCA^T
PflFI 227 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3676 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 4688 GTTTAAAC GTTT^AAAC
PpuMI 3316 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1532 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4792 ATTAAT AT^TAAT
RcaI 622 TCATGA T^CATGA
SapI 1430 GAAGAGC GCTCTTC(1/4)
SbfI 3516 CCTGCAGG CCTGCA^GG
SfoI 1218 GGCGCC GGC^GCC
SnaBI 3981 TACGTA TAC^GTA
SphI 186 GCATGC GCATG^C
Sse8387I 3516 CCTGCAGG CCTGCA^GG
SspI 4341 AATATT AAT^ATT
StuI 443 AGGCCT AGG^CCT
SwaI 2305 ATTTAAAT ATTT^AAAT
Tth111I 227 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3676 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4158 TCTAGA T^CTAGA
XcmI 2058 CCATCTGTGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 26 GTCTAC GT^[AC][GT]AC
3631 GTCTAC
AccII 1106 CGCG CG^CG
3749 CGCG
AccIII 558 TCCGGA T^CCGGA
2355 TCCGGA
AlwNI 1058 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1158 CAGAAGCTG
BalI 645 TGGCCA TGG^CCA
3013 TGGCCA
BanII 1040 GAGCCC G[GA]GC[CT]^C
1873 GGGCTC
BglII 1354 AGATCT A^GATCT
2081 AGATCT
BsaI 1604 GAGACC GGTCTC(1/5)
2420 GGTCTC
BsaWI 558 TCCGGA [AT]^CCGG[AT]
2355 TCCGGA
BseAI 558 TCCGGA T^CCGGA
2355 TCCGGA
BspEI 558 TCCGGA T^CCGGA
2355 TCCGGA
BstUI 1106 CGCG CG^CG
3749 CGCG
DraIII 1412 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
1649 CACAAGGTG
HgaI 4482 GACGC GACGC(5/10)
582 GCGTC
MluNI 645 TGGCCA TGG^CCA
3013 TGGCCA
MroI 558 TCCGGA T^CCGGA
2355 TCCGGA
MscI 645 TGGCCA TGG^CCA
3013 TGGCCA
MvnI 1106 CGCG CG^CG
3749 CGCG
NcoI 913 CCATGG C^CATGG
3950 CCATGG
NdeI 725 CATATG CA^TATG
3082 CATATG
PstI 2561 CTGCAG CTGCA^G
3517 CTGCAG
SfcI 2561 CTGCAG C^T[GA][CT]AG
3517 CTGCAG
TfiI 1066 GAATC G^A[AT]TC
4166 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
AvrII BanI BbrPI BbsI BbvI
BcnI BfaI BlnI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI