##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbrPI BcgI
BclI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BspHI BspLU11I BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FbaI FseI
FspI HpaI KpnI MamI MluI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
TfiI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1857 GTCTAC GT^[AC][GT]AC
AccIII 2081 TCCGGA T^CCGGA
AcyI 316 GGCGCC G[GA]^CG[CT]C
AflII 2188 CTTAAG C^TTAAG
Alw44I 2739 GTGCAC G^TGCAC
ApaLI 2739 GTGCAC G^TGCAC
BbeI 316 GGCGCC GGCGC^C
BbsI 3061 GTCTTC GAAGAC(2/6)
BciVI 1952 GTATCC GTATCC(6/5)
BfrI 2188 CTTAAG C^TTAAG
BglI 213 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3061 GTCTTC GAAGAC(2/6)
BsaHI 316 GGCGCC G[GA]^CG[CT]C
BsaI 1285 GAGACC GGTCTC(1/5)
BseAI 2081 TCCGGA T^CCGGA
Bsp1407I 2954 TGTACA T^GTACA
BspDI 2222 ATCGAT AT^CGAT
BspEI 2081 TCCGGA T^CCGGA
BsrGI 2954 TGTACA T^GTACA
BssSI 55 CTCGTG CACGAG(-5/-1)
BstXI 1380 CCACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2222 ATCGAT AT^CGAT
DraI 1867 TTTAAA TTT^AAA
DraIII 2595 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1848 GACAGAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3376 GAATTC G^AATTC
EcoT22I 400 ATGCAT ATGCA^T
HgaI 2861 GCGTC GACGC(5/10)
Hin1I 316 GGCGCC G[GA]^CG[CT]C
HindIII 1178 AAGCTT A^AGCTT
KasI 316 GGCGCC G^GCGCC
KspI 193 CCGCGG CCGC^GG
MfeI 3335 CAATTG C^AATTG
MroI 2081 TCCGGA T^CCGGA
MunI 3335 CAATTG C^AATTG
NarI 316 GGCGCC GG^CGCC
NsiI 400 ATGCAT ATGCA^T
PaeR7I 249 CTCGAG C^TCGAG
PshAI 1481 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3321 CAGCTG CAG^CTG
SacII 193 CCGCGG CCGC^GG
SfoI 316 GGCGCC GGC^GCC
SpeI 1015 ACTAGT A^CTAGT
SphI 2603 GCATGC GCATG^C
SspBI 2954 TGTACA T^GTACA
XhoI 249 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1457 AAATTC [GA]^AATT[CT]
3376 GAATTC
Aor51HI 105 AGCGCT AGC^GCT
2387 AGCGCT
ApoI 1457 AAATTC [GA]^AATT[CT]
3376 GAATTC
AspI 2143 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2431 GACTGGGTC
BbvCI 646 CCTCAGC CCTCAGC(-5/-2)
1003 GCTGAGG
BlpI 268 GCTGAGC GC^T[ACGT]AGC
449 GCTCAGC
Bpu1102I 268 GCTGAGC GC^T[ACGT]AGC
449 GCTCAGC
BsaWI 722 ACCGGA [AT]^CCGG[AT]
2081 TCCGGA
BsmI 2096 GAATGC GAATGC(1/-1)
3405 GAATGC
BspMI 899 ACCTGC ACCTGC(4/8)
1288 ACCTGC
BsrFI 525 GCCGGT [GA]^CCGG[CT]
1382 ACCGGC
CelII 268 GCTGAGC GC^T[ACGT]AGC
449 GCTCAGC
Cfr10I 525 GCCGGT [GA]^CCGG[CT]
1382 ACCGGC
Eco47III 105 AGCGCT AGC^GCT
2387 AGCGCT
HincII 1402 GTTGAC GT[CT]^[GA]AC
1837 GTCAAC
HindII 1402 GTTGAC GT[CT]^[GA]AC
1837 GTCAAC
MaeII 837 ACGT A^CGT
2546 ACGT
MslI 2065 CACTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2598 CATGTGCATG
PflFI 2143 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2431 GACTGGGTC
PpuMI 783 AGGTCCC [GA]G^G[AT]CC[CT]
1156 GGGTCCC
Tth111I 2143 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2431 GACTGGGTC
XcmI 139 CCACTCCCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2078 CCATCCGGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI XmnI