##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AhdI AscI AseI AsnI
AspEI AspI AvrII BamHI BcgI
BglI BlnI BsaBI BseAI BsiWI
BspDI BspEI BstEII BstPI BstXI
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRV
FseI MamI MfeI MluI MroI
MunI NheI NotI NruI PacI
PflFI PflMI PmeI PshAI PshBI
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1198 GACGTC GACGT^C
AclI 3116 AACGTT AA^CGTT
AgeI 1981 ACCGGT A^CCGGT
AocI 3355 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3216 AGCGCT AGC^GCT
ApaI 4519 GGGCCC GGGCC^C
Asp700I 4198 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3518 TGCGCA TGC^GCA
BbeI 231 GGCGCC GGCGC^C
BbvCI 706 CCTCAGC CCTCAGC(-5/-2)
BclI 2382 TGATCA T^GATCA
BsiEI 1281 CGGTCG CG[GA][CT]^CG
BsmI 1118 GAATGC GAATGC(1/-1)
Bst1107I 4347 GTATAC GTA^TAC
BstBI 4064 TTCGAA TT^CGAA
BstZ17I 4347 GTATAC GTA^TAC
Bsu36I 3355 CCTGAGG CC^T[ACGT]AGG
DraIII 2813 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2513 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3216 AGCGCT AGC^GCT
Eco81I 3355 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1601 ATGCAT ATGCA^T
FbaI 2382 TGATCA T^GATCA
FspI 3518 TGCGCA TGC^GCA
HindIII 4873 AAGCTT A^AGCTT
HpaI 3232 GTTAAC GTT^AAC
KasI 231 GGCGCC G^GCGCC
NaeI 3599 GCCGGC GCC^GGC
NarI 231 GGCGCC GG^CGCC
NgoMIV 3599 GCCGGC G^CCGGC
NsiI 1601 ATGCAT ATGCA^T
NspV 4064 TTCGAA TT^CGAA
PaeR7I 3124 CTCGAG C^TCGAG
PinAI 1981 ACCGGT A^CCGGT
Psp1406I 3116 AACGTT AA^CGTT
SacI 3089 GAGCTC GAGCT^C
SapI 1904 GAAGAGC GCTCTTC(1/4)
SfoI 231 GGCGCC GGC^GCC
SfuI 4064 TTCGAA TT^CGAA
SspI 2026 AATATT AAT^ATT
XhoI 3124 CTCGAG C^TCGAG
XmnI 4198 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 114 GGTACC G^GTACC
4079 GGTACC
AlwNI 2752 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3503 CAGAAACTG
Asp718I 114 GGTACC G^GTACC
4079 GGTACC
BalI 575 TGGCCA TGG^CCA
3490 TGGCCA
BbrPI 2363 CACGTG CAC^GTG
2627 CACGTG
BciVI 3274 GGATAC GTATCC(6/5)
4760 GGATAC
BglII 3553 AGATCT A^GATCT
4170 AGATCT
BlpI 1478 GCTCAGC GC^T[ACGT]AGC
2885 GCTGAGC
Bpu1102I 1478 GCTCAGC GC^T[ACGT]AGC
2885 GCTGAGC
BsaAI 2363 CACGTG [CT]AC^GT[GA]
2627 CACGTG
BsaWI 1981 ACCGGT [AT]^CCGG[AT]
3278 ACCGGA
BsmBI 1196 GAGACG CGTCTC(1/5)
3011 GAGACG
Bsp1407I 2012 TGTACA T^GTACA
4636 TGTACA
BspHI 988 TCATGA T^CATGA
1206 TCATGA
BsrFI 1981 ACCGGT [GA]^CCGG[CT]
3599 GCCGGC
BsrGI 2012 TGTACA T^GTACA
4636 TGTACA
BssHII 151 GCGCGC G^CGCGC
249 GCGCGC
CelII 1478 GCTCAGC GC^T[ACGT]AGC
2885 GCTGAGC
Cfr10I 1981 ACCGGT [GA]^CCGG[CT]
3599 GCCGGC
EcoRI 2333 GAATTC G^AATTC
3361 GAATTC
HincII 545 GTTGAC GT[CT]^[GA]AC
3232 GTTAAC
HindII 545 GTTGAC GT[CT]^[GA]AC
3232 GTTAAC
KpnI 114 GGTACC GGTAC^C
4079 GGTACC
MluNI 575 TGGCCA TGG^CCA
3490 TGGCCA
MscI 575 TGGCCA TGG^CCA
3490 TGGCCA
NdeI 977 CATATG CA^TATG
3585 CATATG
PmaCI 2363 CACGTG CAC^GTG
2627 CACGTG
PmlI 2363 CACGTG CAC^GTG
2627 CACGTG
RcaI 988 TCATGA T^CATGA
1206 TCATGA
SspBI 2012 TGTACA T^GTACA
4636 TGTACA
XcmI 529 CCAGCTGACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3546 CCAAGCAAGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI