##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AclI Aor51HI ApaI AscI
AviII BalI BcgI BseAI BsiWI
BsmBI BspDI BspEI BspMI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I FseI FspI HgaI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NcoI NgoMIV NotI
NruI NspV PacI PmeI Psp1406I
PvuI RsrII SacII SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
Sse8387I StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 3785 GACGTC GACGT^C
Acc65I 2680 GGTACC G^GTACC
AflII 4330 CTTAAG C^TTAAG
AgeI 7 ACCGGT A^CCGGT
AhdI 2291 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1984 CCTCAGG CC^T[ACGT]AGG
Asp700I 2330 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2680 GGTACC G^GTACC
AspEI 2291 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2294 CCTAGG C^CTAGG
BamHI 1513 GGATCC G^GATCC
BbeI 1970 GGCGCC GGCGC^C
BbrPI 2831 CACGTG CAC^GTG
BbsI 1621 GAAGAC GAAGAC(2/6)
BciVI 861 GTATCC GTATCC(6/5)
BfrI 4330 CTTAAG C^TTAAG
BglI 2644 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2294 CCTAGG C^CTAGG
BlpI 4128 GCTTAGC GC^T[ACGT]AGC
BpmI 2065 CTCCAG CTGGAG(16/14)
Bpu1102I 4128 GCTTAGC GC^T[ACGT]AGC
BpuAI 1621 GAAGAC GAAGAC(2/6)
BsaBI 1868 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 738 CGACCG CG[GA][CT]^CG
Bsp1407I 3949 TGTACA T^GTACA
BsrFI 7 ACCGGT [GA]^CCGG[CT]
BsrGI 3949 TGTACA T^GTACA
BstAPI 2519 GCAATTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1984 CCTCAGG CC^T[ACGT]AGG
CelII 4128 GCTTAGC GC^T[ACGT]AGC
Cfr10I 7 ACCGGT [GA]^CCGG[CT]
DraIII 4644 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2291 GACCCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1984 CCTCAGG CC^T[ACGT]AGG
EcoNI 2748 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 832 GATATC GAT^ATC
EcoT22I 4183 ATGCAT ATGCA^T
HindIII 1589 AAGCTT A^AGCTT
HpaI 4309 GTTAAC GTT^AAC
KasI 1970 GGCGCC G^GCGCC
KpnI 2680 GGTACC GGTAC^C
MamI 1868 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1970 GGCGCC GG^CGCC
NheI 1025 GCTAGC G^CTAGC
NsiI 4183 ATGCAT ATGCA^T
PaeR7I 808 CTCGAG C^TCGAG
PinAI 7 ACCGGT A^CCGGT
PmaCI 2831 CACGTG CAC^GTG
PmlI 2831 CACGTG CAC^GTG
PshAI 2461 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 315 GAGCTC GAGCT^C
SalI 2213 GTCGAC G^TCGAC
SapI 355 GCTCTTC GCTCTTC(1/4)
SfoI 1970 GGCGCC GGC^GCC
SgrAI 6 CACCGGTG C[GA]^CCGG[CT]G
SphI 4838 GCATGC GCATG^C
SspBI 3949 TGTACA T^GTACA
XhoI 808 CTCGAG C^TCGAG
XmnI 2330 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2213 GTCGAC GT^[AC][GT]AC
4354 GTAGAC
AcyI 1970 GGCGCC G[GA]^CG[CT]C
3785 GACGTC
Alw44I 4475 GTGCAC G^TGCAC
4730 GTGCAC
AlwNI 1860 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
2436 CAGAATCTG
ApaLI 4475 GTGCAC G^TGCAC
4730 GTGCAC
AseI 785 ATTAAT AT^TAAT
5047 ATTAAT
AsnI 785 ATTAAT AT^TAAT
5047 ATTAAT
AspI 114 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2108 GACAATGTC
BanII 315 GAGCTC G[GA]GC[CT]^C
353 GGGCTC
BbvCI 3266 CCTCAGC CCTCAGC(-5/-2)
1706 GCTGAGG
BsaHI 1970 GGCGCC G[GA]^CG[CT]C
3785 GACGTC
BsaWI 7 ACCGGT [AT]^CCGG[AT]
644 ACCGGA
BspHI 1999 TCATGA T^CATGA
4494 TCATGA
BstEII 2812 GGTGACC G^GT[ACGT]ACC
3536 GGTTACC
BstPI 2812 GGTGACC G^GT[ACGT]ACC
3536 GGTTACC
DraI 1610 TTTAAA TTT^AAA
3985 TTTAAA
DrdI 4665 GACAGATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4774 GACTGCATTGTC
EcoO65I 2812 GGTGACC G^GT[ACGT]ACC
3536 GGTTACC
Hin1I 1970 GGCGCC G[GA]^CG[CT]C
3785 GACGTC
HincII 2213 GTCGAC GT[CT]^[GA]AC
4309 GTTAAC
HindII 2213 GTCGAC GT[CT]^[GA]AC
4309 GTTAAC
NdeI 1626 CATATG CA^TATG
4870 CATATG
PflFI 114 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2108 GACAATGTC
PpuMI 1384 GGGTCCC [GA]G^G[AT]CC[CT]
3602 GGGACCC
PshBI 785 ATTAAT AT^TAAT
5047 ATTAAT
PvuII 752 CAGCTG CAG^CTG
2245 CAGCTG
RcaI 1999 TCATGA T^CATGA
4494 TCATGA
SmaI 65 CCCGGG CCC^GGG
4392 CCCGGG
SmlI 808 CTCGAG C^T[CT][GA]AG
4330 CTTAAG
SspI 2952 AATATT AAT^ATT
3925 AATATT
Tth111I 114 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2108 GACAATGTC
XmaCI 65 CCCGGG C^CCGGG
4392 CCCGGG
XmaI 65 CCCGGG C^CCGGG
4392 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BbvI BclI BcnI
BfaI BglII BmyI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII