##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI Asp700I
AspEI AviII BamHI BbrPI BciVI
BclI BfrI BsaAI BsiWI Bsp1407I
BspDI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DrdI
Eam1105I Eco47III EcoO65I EcoRI FbaI
FspI KspI MfeI MluI MunI
NheI NruI PacI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SapI SgrAI
SmaI SnaBI SpeI SphI SspBI
StuI SwaI Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3234 GGTACC G^GTACC
AccI 1073 GTCGAC GT^[AC][GT]AC
AccIII 1112 TCCGGA T^CCGGA
AflIII 678 ACATGT A^C[GA][CT]GT
Alw44I 2320 GTGCAC G^TGCAC
AocI 3328 CCTCAGG CC^T[ACGT]AGG
ApaLI 2320 GTGCAC G^TGCAC
AseI 2061 ATTAAT AT^TAAT
AsnI 2061 ATTAAT AT^TAAT
Asp718I 3234 GGTACC G^GTACC
AvaI 653 CTCGAG C^[CT]CG[GA]G
BbeI 505 GGCGCC GGCGC^C
BglI 1366 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 144 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2917 GGTCTC GGTCTC(1/5)
BseAI 1112 TCCGGA T^CCGGA
BsmBI 3678 CGTCTC CGTCTC(1/5)
BsmI 1527 GAATGC GAATGC(1/-1)
BsoBI 653 CTCGAG C^[CT]CG[GA]G
BspEI 1112 TCCGGA T^CCGGA
BspHI 2705 TCATGA T^CATGA
BspLU11I 678 ACATGT A^CATGT
BspMI 2437 GCAGGT ACCTGC(4/8)
BsrBI 1453 GAGCGG CCGCTC(-3/-3)
BssSI 1462 CACGAG CACGAG(-5/-1)
BstBI 863 TTCGAA TT^CGAA
Bsu36I 3328 CCTCAGG CC^T[ACGT]AGG
DraI 193 TTTAAA TTT^AAA
DraIII 1125 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3328 CCTCAGG CC^T[ACGT]AGG
EcoRV 174 GATATC GAT^ATC
FseI 105 GGCCGGCC GGCCGG^CC
HindIII 1425 AAGCTT A^AGCTT
HpaI 3727 GTTAAC GTT^AAC
KasI 505 GGCGCC G^GCGCC
KpnI 3234 GGTACC GGTAC^C
MaeII 3312 ACGT A^CGT
MamI 144 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1112 TCCGGA T^CCGGA
NarI 505 GGCGCC GG^CGCC
NotI 121 GCGGCCGC GC^GGCCGC
NspV 863 TTCGAA TT^CGAA
PaeR7I 653 CTCGAG C^TCGAG
PshAI 2668 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2061 ATTAAT AT^TAAT
RcaI 2705 TCATGA T^CATGA
SalI 1073 GTCGAC G^TCGAC
SbfI 1837 CCTGCAGG CCTGCA^GG
SfiI 1365 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 505 GGCGCC GGC^GCC
SfuI 863 TTCGAA TT^CGAA
Sse8387I 1837 CCTGCAGG CCTGCA^GG
SspI 3173 AATATT AAT^ATT
XhoI 653 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwI 457 GATCC GGATC(4/5)
2642 GATCC
ApaI 1882 GGGCCC GGGCC^C
2257 GGGCCC
AspI 95 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1336 GACATTGTC
AvrII 3145 CCTAGG C^CTAGG
3230 CCTAGG
BalI 2805 TGGCCA TGG^CCA
2944 TGGCCA
BanI 505 GGCGCC G^G[CT][GA]CC
3234 GGTACC
BbvCI 2234 CCTCAGC CCTCAGC(-5/-2)
3618 CCTCAGC
BcgI 1030 CGAGTGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1035 GCAGTGCAATCG
BglII 188 AGATCT A^GATCT
3051 AGATCT
BlnI 3145 CCTAGG C^CTAGG
3230 CCTAGG
BsaWI 1112 TCCGGA [AT]^CCGG[AT]
1600 TCCGGT
BsiEI 104 CGGCCG CG[GA][CT]^CG
122 CGGCCG
BsrFI 42 GCCGGC [GA]^CCGG[CT]
106 GCCGGC
BstYI 188 AGATCT [GA]^GATC[CT]
3051 AGATCT
Cfr10I 42 GCCGGC [GA]^CCGG[CT]
106 GCCGGC
DsaI 995 CCATGG C^C[GA][CT]GG
3073 CCATGG
EagI 104 CGGCCG C^GGCCG
122 CGGCCG
EclXI 104 CGGCCG C^GGCCG
122 CGGCCG
Eco52I 104 CGGCCG C^GGCCG
122 CGGCCG
EcoT22I 223 ATGCAT ATGCA^T
1583 ATGCAT
HaeII 505 GGCGCC [GA]GCGC^[CT]
926 GGCGCT
MflI 188 AGATCT [GA]^GATC[CT]
3051 AGATCT
MluNI 2805 TGGCCA TGG^CCA
2944 TGGCCA
MscI 2805 TGGCCA TGG^CCA
2944 TGGCCA
NaeI 42 GCCGGC GCC^GGC
106 GCCGGC
NcoI 995 CCATGG C^CATGG
3073 CCATGG
NgoMIV 42 GCCGGC G^CCGGC
106 GCCGGC
NsiI 223 ATGCAT ATGCA^T
1583 ATGCAT
NspI 678 ACATGT [GA]CATG^[CT]
1229 ACATGC
PflFI 95 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1336 GACATTGTC
PpuMI 383 AGGACCC [GA]G^G[AT]CC[CT]
3129 AGGACCT
ScaI 1301 AGTACT AGT^ACT
1385 AGTACT
SexAI 96 ACCTGGT A^CC[AT]GGT
3023 ACCTGGT
Tth111I 95 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1336 GACATTGTC
XhoII 188 AGATCT [GA]^GATC[CT]
3051 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI