################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccI AccIII AclI AgeI AscI Asp700I AvrII BcgI BciVI BclI BlnI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI MamI MfeI MroI MunI NotI NruI NsiI NspV PacI PflMI PinAI PmeI Psp1406I PvuI RsrII SalI SapI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Van91I XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflII 2683 CTTAAG C^TTAAG Alw44I 2245 GTGCAC G^TGCAC ApaLI 2245 GTGCAC G^TGCAC AseI 4190 ATTAAT AT^TAAT AsnI 4190 ATTAAT AT^TAAT AspI 4769 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 471 GGCGCC GGCGC^C BbrPI 3175 CACGTG CAC^GTG BfrI 2683 CTTAAG C^TTAAG BglII 765 AGATCT A^GATCT BsiEI 3186 CGGCCG CG[GA][CT]^CG Bsp1407I 2855 TGTACA T^GTACA BspHI 2757 TCATGA T^CATGA BsrGI 2855 TGTACA T^GTACA BssSI 3432 CACGAG CACGAG(-5/-1) EagI 3186 CGGCCG C^GGCCG EclXI 3186 CGGCCG C^GGCCG Eco52I 3186 CGGCCG C^GGCCG HindIII 5013 AAGCTT A^AGCTT KasI 471 GGCGCC G^GCGCC MluI 3871 ACGCGT A^CGCGT NarI 471 GGCGCC GG^CGCC NdeI 3293 CATATG CA^TATG NheI 2178 GCTAGC G^CTAGC PaeR7I 430 CTCGAG C^TCGAG PflFI 4769 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3175 CACGTG CAC^GTG PmlI 3175 CACGTG CAC^GTG PshBI 4190 ATTAAT AT^TAAT RcaI 2757 TCATGA T^CATGA SexAI 3394 ACCAGGT A^CC[AT]GGT SfiI 4828 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 471 GGCGCC GGC^GCC SspBI 2855 TGTACA T^GTACA Tth111I 4769 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 430 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 4250 GGTACC G^GTACC 4691 GGTACC AcsI 2503 GAATTT [GA]^AATT[CT] 2993 GAATTT AcyI 471 GGCGCC G[GA]^CG[CT]C 2355 GGCGTC Aor51HI 2537 AGCGCT AGC^GCT 3602 AGCGCT ApoI 2503 GAATTT [GA]^AATT[CT] 2993 GAATTT Asp718I 4250 GGTACC G^GTACC 4691 GGTACC AviII 3047 TGCGCA TGC^GCA 3215 TGCGCA BamHI 4260 GGATCC G^GATCC 4515 GGATCC BsaAI 2284 CACGTA [CT]AC^GT[GA] 3175 CACGTG BsaHI 471 GGCGCC G[GA]^CG[CT]C 2355 GGCGTC BsmBI 1777 CGTCTC CGTCTC(1/5) 3874 CGTCTC DraI 4202 TTTAAA TTT^AAA 5187 TTTAAA DrdI 3334 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3352 GACACCTTTGTC Eco47III 2537 AGCGCT AGC^GCT 3602 AGCGCT FspI 3047 TGCGCA TGC^GCA 3215 TGCGCA Hin1I 471 GGCGCC G[GA]^CG[CT]C 2355 GGCGTC HincII 364 GTCAAC GT[CT]^[GA]AC 3382 GTTGAC HindII 364 GTCAAC GT[CT]^[GA]AC 3382 GTTGAC KpnI 4250 GGTACC GGTAC^C 4691 GGTACC NaeI 176 GCCGGC GCC^GGC 1979 GCCGGC NcoI 4292 CCATGG C^CATGG 4605 CCATGG NgoMIV 176 GCCGGC G^CCGGC 1979 GCCGGC ScaI 611 AGTACT AGT^ACT 4873 AGTACT XbaI 768 TCTAGA T^CTAGA 4079 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApaI AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI