##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AscI Asp700I AvrII BcgI BciVI
BclI BlnI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI MamI MfeI MroI
MunI NotI NruI NsiI NspV
PacI PflMI PinAI PmeI Psp1406I
PvuI RsrII SalI SapI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI Van91I XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 2683 CTTAAG C^TTAAG
Alw44I 2245 GTGCAC G^TGCAC
ApaLI 2245 GTGCAC G^TGCAC
AseI 4190 ATTAAT AT^TAAT
AsnI 4190 ATTAAT AT^TAAT
AspI 4769 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 471 GGCGCC GGCGC^C
BbrPI 3175 CACGTG CAC^GTG
BfrI 2683 CTTAAG C^TTAAG
BglII 765 AGATCT A^GATCT
BsiEI 3186 CGGCCG CG[GA][CT]^CG
Bsp1407I 2855 TGTACA T^GTACA
BspHI 2757 TCATGA T^CATGA
BsrGI 2855 TGTACA T^GTACA
BssSI 3432 CACGAG CACGAG(-5/-1)
EagI 3186 CGGCCG C^GGCCG
EclXI 3186 CGGCCG C^GGCCG
Eco52I 3186 CGGCCG C^GGCCG
HindIII 5013 AAGCTT A^AGCTT
KasI 471 GGCGCC G^GCGCC
MluI 3871 ACGCGT A^CGCGT
NarI 471 GGCGCC GG^CGCC
NdeI 3293 CATATG CA^TATG
NheI 2178 GCTAGC G^CTAGC
PaeR7I 430 CTCGAG C^TCGAG
PflFI 4769 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3175 CACGTG CAC^GTG
PmlI 3175 CACGTG CAC^GTG
PshBI 4190 ATTAAT AT^TAAT
RcaI 2757 TCATGA T^CATGA
SexAI 3394 ACCAGGT A^CC[AT]GGT
SfiI 4828 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 471 GGCGCC GGC^GCC
SspBI 2855 TGTACA T^GTACA
Tth111I 4769 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 430 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 4250 GGTACC G^GTACC
4691 GGTACC
AcsI 2503 GAATTT [GA]^AATT[CT]
2993 GAATTT
AcyI 471 GGCGCC G[GA]^CG[CT]C
2355 GGCGTC
Aor51HI 2537 AGCGCT AGC^GCT
3602 AGCGCT
ApoI 2503 GAATTT [GA]^AATT[CT]
2993 GAATTT
Asp718I 4250 GGTACC G^GTACC
4691 GGTACC
AviII 3047 TGCGCA TGC^GCA
3215 TGCGCA
BamHI 4260 GGATCC G^GATCC
4515 GGATCC
BsaAI 2284 CACGTA [CT]AC^GT[GA]
3175 CACGTG
BsaHI 471 GGCGCC G[GA]^CG[CT]C
2355 GGCGTC
BsmBI 1777 CGTCTC CGTCTC(1/5)
3874 CGTCTC
DraI 4202 TTTAAA TTT^AAA
5187 TTTAAA
DrdI 3334 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3352 GACACCTTTGTC
Eco47III 2537 AGCGCT AGC^GCT
3602 AGCGCT
FspI 3047 TGCGCA TGC^GCA
3215 TGCGCA
Hin1I 471 GGCGCC G[GA]^CG[CT]C
2355 GGCGTC
HincII 364 GTCAAC GT[CT]^[GA]AC
3382 GTTGAC
HindII 364 GTCAAC GT[CT]^[GA]AC
3382 GTTGAC
KpnI 4250 GGTACC GGTAC^C
4691 GGTACC
NaeI 176 GCCGGC GCC^GGC
1979 GCCGGC
NcoI 4292 CCATGG C^CATGG
4605 CCATGG
NgoMIV 176 GCCGGC G^CCGGC
1979 GCCGGC
ScaI 611 AGTACT AGT^ACT
4873 AGTACT
XbaI 768 TCTAGA T^CTAGA
4079 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AfaI AflIII AhdI
AluI AlwI AlwNI AocI ApaI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI