##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AscI        Asp700I     AvrII       BcgI        BciVI       
BclI        BlnI        BsaBI       BsaWI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BspLU11I    
BsrDI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraIII      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HpaI        MamI        MfeI        MroI        
MunI        NotI        NruI        NsiI        NspV        
PacI        PflMI       PinAI       PmeI        Psp1406I    
PvuI        RsrII       SalI        SapI        SbfI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        Van91I      XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AflII        2683  CTTAAG               C^TTAAG
Alw44I       2245  GTGCAC               G^TGCAC
ApaLI        2245  GTGCAC               G^TGCAC
AseI         4190  ATTAAT               AT^TAAT
AsnI         4190  ATTAAT               AT^TAAT
AspI         4769  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          471  GGCGCC               GGCGC^C
BbrPI        3175  CACGTG               CAC^GTG
BfrI         2683  CTTAAG               C^TTAAG
BglII         765  AGATCT               A^GATCT
BsiEI        3186  CGGCCG               CG[GA][CT]^CG
Bsp1407I     2855  TGTACA               T^GTACA
BspHI        2757  TCATGA               T^CATGA
BsrGI        2855  TGTACA               T^GTACA
BssSI        3432  CACGAG               CACGAG(-5/-1)
EagI         3186  CGGCCG               C^GGCCG
EclXI        3186  CGGCCG               C^GGCCG
Eco52I       3186  CGGCCG               C^GGCCG
HindIII      5013  AAGCTT               A^AGCTT
KasI          471  GGCGCC               G^GCGCC
MluI         3871  ACGCGT               A^CGCGT
NarI          471  GGCGCC               GG^CGCC
NdeI         3293  CATATG               CA^TATG
NheI         2178  GCTAGC               G^CTAGC
PaeR7I        430  CTCGAG               C^TCGAG
PflFI        4769  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3175  CACGTG               CAC^GTG
PmlI         3175  CACGTG               CAC^GTG
PshBI        4190  ATTAAT               AT^TAAT
RcaI         2757  TCATGA               T^CATGA
SexAI        3394  ACCAGGT              A^CC[AT]GGT
SfiI         4828  GGCCATCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          471  GGCGCC               GGC^GCC
SspBI        2855  TGTACA               T^GTACA
Tth111I      4769  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          430  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I       4250  GGTACC               G^GTACC
             4691  GGTACC              
AcsI         2503  GAATTT               [GA]^AATT[CT]
             2993  GAATTT              
AcyI          471  GGCGCC               G[GA]^CG[CT]C
             2355  GGCGTC              
Aor51HI      2537  AGCGCT               AGC^GCT
             3602  AGCGCT              
ApoI         2503  GAATTT               [GA]^AATT[CT]
             2993  GAATTT              
Asp718I      4250  GGTACC               G^GTACC
             4691  GGTACC              
AviII        3047  TGCGCA               TGC^GCA
             3215  TGCGCA              
BamHI        4260  GGATCC               G^GATCC
             4515  GGATCC              
BsaAI        2284  CACGTA               [CT]AC^GT[GA]
             3175  CACGTG              
BsaHI         471  GGCGCC               G[GA]^CG[CT]C
             2355  GGCGTC              
BsmBI        1777  CGTCTC               CGTCTC(1/5)
             3874  CGTCTC              
DraI         4202  TTTAAA               TTT^AAA
             5187  TTTAAA              
DrdI         3334  GACATGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3352  GACACCTTTGTC        
Eco47III     2537  AGCGCT               AGC^GCT
             3602  AGCGCT              
FspI         3047  TGCGCA               TGC^GCA
             3215  TGCGCA              
Hin1I         471  GGCGCC               G[GA]^CG[CT]C
             2355  GGCGTC              
HincII        364  GTCAAC               GT[CT]^[GA]AC
             3382  GTTGAC              
HindII        364  GTCAAC               GT[CT]^[GA]AC
             3382  GTTGAC              
KpnI         4250  GGTACC               GGTAC^C
             4691  GGTACC              
NaeI          176  GCCGGC               GCC^GGC
             1979  GCCGGC              
NcoI         4292  CCATGG               C^CATGG
             4605  CCATGG              
NgoMIV        176  GCCGGC               G^CCGGC
             1979  GCCGGC              
ScaI          611  AGTACT               AGT^ACT
             4873  AGTACT              
XbaI          768  TCTAGA               T^CTAGA
             4079  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AhdI        
AluI        AlwI        AlwNI       AocI        ApaI        
AspEI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eam1105I    EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PshAI       PstI        PvuII       RsaI        
SacI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        SphI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmaCI       XmaI