##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Alw44I ApaLI AscI Asp718I AspEI
AviII AvrII BlnI BsiEI BsiWI
BsmBI BsmI BspDI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV FseI FspI HpaI KpnI
KspI MluI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2199 GACGTC GACGT^C
AccIII 83 TCCGGA T^CCGGA
AflII 785 CTTAAG C^TTAAG
AflIII 2772 ACACGT A^C[GA][CT]GT
Aor51HI 3885 AGCGCT AGC^GCT
ApaI 1391 GGGCCC GGGCC^C
AseI 4617 ATTAAT AT^TAAT
AsnI 4617 ATTAAT AT^TAAT
Asp700I 3829 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 374 GGATCC G^GATCC
BbrPI 2773 CACGTG CAC^GTG
BclI 2243 TGATCA T^GATCA
BfrI 785 CTTAAG C^TTAAG
BsaI 2713 GGTCTC GGTCTC(1/5)
BsaWI 83 TCCGGA [AT]^CCGG[AT]
BseAI 83 TCCGGA T^CCGGA
BspEI 83 TCCGGA T^CCGGA
BspHI 4338 TCATGA T^CATGA
BssSI 1694 CACGAG CACGAG(-5/-1)
BstEII 3428 GGTCACC G^GT[ACGT]ACC
BstPI 3428 GGTCACC G^GT[ACGT]ACC
DrdI 3747 GACAATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3885 AGCGCT AGC^GCT
EcoNI 535 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3428 GGTCACC G^GT[ACGT]ACC
FbaI 2243 TGATCA T^GATCA
HindIII 675 AAGCTT A^AGCTT
MfeI 2780 CAATTG C^AATTG
MroI 83 TCCGGA T^CCGGA
MunI 2780 CAATTG C^AATTG
NdeI 2315 CATATG CA^TATG
NheI 2358 GCTAGC G^CTAGC
PmaCI 2773 CACGTG CAC^GTG
PmlI 2773 CACGTG CAC^GTG
PshBI 4617 ATTAAT AT^TAAT
RcaI 4338 TCATGA T^CATGA
ScaI 4493 AGTACT AGT^ACT
SfiI 3631 GGCCGGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 125 CCCGGG CCC^GGG
StuI 3639 AGGCCT AGG^CCT
XbaI 2872 TCTAGA T^CTAGA
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
XmnI 3829 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 2605 AAATTC [GA]^AATT[CT]
4627 AAATTT
ApoI 2605 AAATTC [GA]^AATT[CT]
4627 AAATTT
AspHI 3104 GAGCAC G[AT]GC[AT]^C
4258 GTGCTC
AvaI 125 CCCGGG C^[CT]CG[GA]G
1524 CCCGAG
BbeI 161 GGCGCC GGCGC^C
1422 GGCGCC
BcgI 1880 CGAGGCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1492 GCAGTTCTGTCG
BglI 2039 GCCTGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3632 GCCGGACAGGC
BglII 890 AGATCT A^GATCT
1012 AGATCT
BlpI 187 GCTCAGC GC^T[ACGT]AGC
623 GCTCAGC
Bpu1102I 187 GCTCAGC GC^T[ACGT]AGC
623 GCTCAGC
BsaAI 593 TACGTG [CT]AC^GT[GA]
2773 CACGTG
BsaBI 1135 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3073 GATGTGCATC
BsiHKAI 3104 GAGCAC G[AT]GC[AT]^C
4258 GTGCTC
BsoBI 125 CCCGGG C^[CT]CG[GA]G
1524 CCCGAG
Bsp1407I 2853 TGTACA T^GTACA
3715 TGTACA
BsrBI 272 GAGCGG CCGCTC(-3/-3)
1182 GAGCGG
BsrGI 2853 TGTACA T^GTACA
3715 TGTACA
CelII 187 GCTCAGC GC^T[ACGT]AGC
623 GCTCAGC
EcoT22I 3503 ATGCAT ATGCA^T
3997 ATGCAT
HincII 554 GTCAAC GT[CT]^[GA]AC
838 GTTGAC
HindII 554 GTCAAC GT[CT]^[GA]AC
838 GTTGAC
KasI 161 GGCGCC G^GCGCC
1422 GGCGCC
MamI 1135 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3073 GATGTGCATC
NarI 161 GGCGCC GG^CGCC
1422 GGCGCC
NsiI 3503 ATGCAT ATGCA^T
3997 ATGCAT
SapI 1179 GAAGAGC GCTCTTC(1/4)
2985 GAAGAGC
SexAI 730 ACCAGGT A^CC[AT]GGT
3942 ACCAGGT
SfoI 161 GGCGCC GGC^GCC
1422 GGCGCC
SphI 637 GCATGC GCATG^C
4474 GCATGC
SspBI 2853 TGTACA T^GTACA
3715 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII