##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI BbeI BbrPI
BbvCI BglII BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoNI EcoRV EcoT22I FseI Hin1I
HincII HindII HpaI KasI KspI
MamI MluI MroI NarI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfiI SfoI SgrAI
SnaBI SpeI StuI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 2929 GGTACC G^GTACC
AccI 2777 GTAGAC GT^[AC][GT]AC
AflII 3664 CTTAAG C^TTAAG
AlwNI 891 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1570 AGCGCT AGC^GCT
ApaI 2822 GGGCCC GGGCC^C
Asp700I 240 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2929 GGTACC G^GTACC
AviII 154 TGCGCA TGC^GCA
AvrII 1634 CCTAGG C^CTAGG
BamHI 1260 GGATCC G^GATCC
BcgI 899 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 120 GGATAC GTATCC(6/5)
BclI 307 TGATCA T^GATCA
BfrI 3664 CTTAAG C^TTAAG
BglI 93 GCCAGTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1634 CCTAGG C^CTAGG
BlpI 1697 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1697 GCTTAGC GC^T[ACGT]AGC
BsaAI 1874 TACGTG [CT]AC^GT[GA]
BsmBI 1681 GAGACG CGTCTC(1/5)
Bsp1407I 2030 TGTACA T^GTACA
BspHI 2770 TCATGA T^CATGA
BsrBI 2148 CCGCTC CCGCTC(-3/-3)
BsrGI 2030 TGTACA T^GTACA
BstAPI 2459 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2020 TTCGAA TT^CGAA
BstEII 3425 GGTGACC G^GT[ACGT]ACC
BstPI 3425 GGTGACC G^GT[ACGT]ACC
BstXI 928 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1697 GCTTAGC GC^T[ACGT]AGC
DrdI 3070 GACTATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1570 AGCGCT AGC^GCT
EcoO65I 3425 GGTGACC G^GT[ACGT]ACC
FbaI 307 TGATCA T^GATCA
FspI 154 TGCGCA TGC^GCA
HgaI 385 GCGTC GACGC(5/10)
HindIII 2024 AAGCTT A^AGCTT
KpnI 2929 GGTACC GGTAC^C
MfeI 3925 CAATTG C^AATTG
MslI 2620 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3925 CAATTG C^AATTG
NaeI 448 GCCGGC GCC^GGC
NcoI 490 CCATGG C^CATGG
NdeI 1987 CATATG CA^TATG
NgoMIV 448 GCCGGC G^CCGGC
NspV 2020 TTCGAA TT^CGAA
RcaI 2770 TCATGA T^CATGA
SapI 2007 GAAGAGC GCTCTTC(1/4)
SbfI 1339 CCTGCAGG CCTGCA^GG
ScaI 1091 AGTACT AGT^ACT
SfuI 2020 TTCGAA TT^CGAA
SmaI 1459 CCCGGG CCC^GGG
SphI 262 GCATGC GCATG^C
Sse8387I 1339 CCTGCAGG CCTGCA^GG
SspBI 2030 TGTACA T^GTACA
XmaCI 1459 CCCGGG C^CCGGG
XmaI 1459 CCCGGG C^CCGGG
XmnI 240 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 384 CGCG CG^CG
2845 CGCG
AvaI 1459 CCCGGG C^[CT]CG[GA]G
2584 CCCGAG
BalI 1750 TGGCCA TGG^CCA
3341 TGGCCA
BseRI 576 CTCCTC GAGGAG(10/8)
3334 CTCCTC
BsoBI 1459 CCCGGG C^[CT]CG[GA]G
2584 CCCGAG
BsrFI 448 GCCGGC [GA]^CCGG[CT]
2070 GCCGGT
BstUI 384 CGCG CG^CG
2845 CGCG
Cfr10I 448 GCCGGC [GA]^CCGG[CT]
2070 GCCGGT
DraIII 1942 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
2695 CACGTCGTG
FauI 1762 CCCGC CCCGC(4/6)
2933 CCCGC
MaeII 1875 ACGT A^CGT
2696 ACGT
MluNI 1750 TGGCCA TGG^CCA
3341 TGGCCA
MscI 1750 TGGCCA TGG^CCA
3341 TGGCCA
MvnI 384 CGCG CG^CG
2845 CGCG
NheI 19 GCTAGC G^CTAGC
3139 GCTAGC
PflMI 1162 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1870 CCAATACGTGG
PvuII 582 CAGCTG CAG^CTG
2034 CAGCTG
SspI 3184 AATATT AAT^ATT
3714 AATATT
TaqI 173 TCGA T^CGA
2021 TCGA
TfiI 1950 GAATC G^A[AT]TC
2767 GAATC
TthHB8I 173 TCGA T^CGA
2021 TCGA
Van91I 1162 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1870 CCAATACGTGG
XcmI 928 CCAAGTTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1603 CCATAGCGCCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII