################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AspI BbeI BbrPI BbvCI BglII BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT22I FseI Hin1I HincII HindII HpaI KasI KspI MamI MluI MroI NarI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SgrAI SnaBI SpeI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### Acc65I 2929 GGTACC G^GTACC AccI 2777 GTAGAC GT^[AC][GT]AC AflII 3664 CTTAAG C^TTAAG AlwNI 891 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1570 AGCGCT AGC^GCT ApaI 2822 GGGCCC GGGCC^C Asp700I 240 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2929 GGTACC G^GTACC AviII 154 TGCGCA TGC^GCA AvrII 1634 CCTAGG C^CTAGG BamHI 1260 GGATCC G^GATCC BcgI 899 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 120 GGATAC GTATCC(6/5) BclI 307 TGATCA T^GATCA BfrI 3664 CTTAAG C^TTAAG BglI 93 GCCAGTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1634 CCTAGG C^CTAGG BlpI 1697 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1697 GCTTAGC GC^T[ACGT]AGC BsaAI 1874 TACGTG [CT]AC^GT[GA] BsmBI 1681 GAGACG CGTCTC(1/5) Bsp1407I 2030 TGTACA T^GTACA BspHI 2770 TCATGA T^CATGA BsrBI 2148 CCGCTC CCGCTC(-3/-3) BsrGI 2030 TGTACA T^GTACA BstAPI 2459 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2020 TTCGAA TT^CGAA BstEII 3425 GGTGACC G^GT[ACGT]ACC BstPI 3425 GGTGACC G^GT[ACGT]ACC BstXI 928 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1697 GCTTAGC GC^T[ACGT]AGC DrdI 3070 GACTATTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1570 AGCGCT AGC^GCT EcoO65I 3425 GGTGACC G^GT[ACGT]ACC FbaI 307 TGATCA T^GATCA FspI 154 TGCGCA TGC^GCA HgaI 385 GCGTC GACGC(5/10) HindIII 2024 AAGCTT A^AGCTT KpnI 2929 GGTACC GGTAC^C MfeI 3925 CAATTG C^AATTG MslI 2620 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 3925 CAATTG C^AATTG NaeI 448 GCCGGC GCC^GGC NcoI 490 CCATGG C^CATGG NdeI 1987 CATATG CA^TATG NgoMIV 448 GCCGGC G^CCGGC NspV 2020 TTCGAA TT^CGAA RcaI 2770 TCATGA T^CATGA SapI 2007 GAAGAGC GCTCTTC(1/4) SbfI 1339 CCTGCAGG CCTGCA^GG ScaI 1091 AGTACT AGT^ACT SfuI 2020 TTCGAA TT^CGAA SmaI 1459 CCCGGG CCC^GGG SphI 262 GCATGC GCATG^C Sse8387I 1339 CCTGCAGG CCTGCA^GG SspBI 2030 TGTACA T^GTACA XmaCI 1459 CCCGGG C^CCGGG XmaI 1459 CCCGGG C^CCGGG XmnI 240 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 384 CGCG CG^CG 2845 CGCG AvaI 1459 CCCGGG C^[CT]CG[GA]G 2584 CCCGAG BalI 1750 TGGCCA TGG^CCA 3341 TGGCCA BseRI 576 CTCCTC GAGGAG(10/8) 3334 CTCCTC BsoBI 1459 CCCGGG C^[CT]CG[GA]G 2584 CCCGAG BsrFI 448 GCCGGC [GA]^CCGG[CT] 2070 GCCGGT BstUI 384 CGCG CG^CG 2845 CGCG Cfr10I 448 GCCGGC [GA]^CCGG[CT] 2070 GCCGGT DraIII 1942 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 2695 CACGTCGTG FauI 1762 CCCGC CCCGC(4/6) 2933 CCCGC MaeII 1875 ACGT A^CGT 2696 ACGT MluNI 1750 TGGCCA TGG^CCA 3341 TGGCCA MscI 1750 TGGCCA TGG^CCA 3341 TGGCCA MvnI 384 CGCG CG^CG 2845 CGCG NheI 19 GCTAGC G^CTAGC 3139 GCTAGC PflMI 1162 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1870 CCAATACGTGG PvuII 582 CAGCTG CAG^CTG 2034 CAGCTG SspI 3184 AATATT AAT^ATT 3714 AATATT TaqI 173 TCGA T^CGA 2021 TCGA TfiI 1950 GAATC G^A[AT]TC 2767 GAATC TthHB8I 173 TCGA T^CGA 2021 TCGA Van91I 1162 CCAATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1870 CCAATACGTGG XcmI 928 CCAAGTTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1603 CCATAGCGCCTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII