##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AvrII BamHI BcgI BfrI BglII
BlnI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FseI
HpaI KspI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI PvuI RsrII SacII
SalI SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2439 GGTACC G^GTACC
AccI 4122 GTAGAC GT^[AC][GT]AC
AclI 2407 AACGTT AA^CGTT
Alw44I 4350 GTGCAC G^TGCAC
ApaLI 4350 GTGCAC G^TGCAC
Asp718I 2439 GGTACC G^GTACC
AspI 3603 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4003 TGCGCA TGC^GCA
BbrPI 595 CACGTG CAC^GTG
BclI 935 TGATCA T^GATCA
BlpI 2664 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2664 GCTGAGC GC^T[ACGT]AGC
BsaBI 1369 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3062 GAATGC GAATGC(1/-1)
BsrFI 23 GCCGGC [GA]^CCGG[CT]
BstXI 4159 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2664 GCTGAGC GC^T[ACGT]AGC
Cfr10I 23 GCCGGC [GA]^CCGG[CT]
DraI 962 TTTAAA TTT^AAA
DraIII 3612 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3045 GACTTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 935 TGATCA T^GATCA
FspI 4003 TGCGCA TGC^GCA
KpnI 2439 GGTACC GGTAC^C
MamI 1369 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 23 GCCGGC GCC^GGC
NcoI 1361 CCATGG C^CATGG
NgoMIV 23 GCCGGC G^CCGGC
NspI 3976 ACATGC [GA]CATG^[CT]
PaeR7I 4062 CTCGAG C^TCGAG
PflFI 3603 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 595 CACGTG CAC^GTG
PmlI 595 CACGTG CAC^GTG
Psp1406I 2407 AACGTT AA^CGTT
ScaI 1290 AGTACT AGT^ACT
SmaI 1640 CCCGGG CCC^GGG
Tth111I 3603 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4062 CTCGAG C^TCGAG
XmaCI 1640 CCCGGG C^CCGGG
XmaI 1640 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 596 ACGTGT A^C[GA][CT]GT
4243 ACGTGT
Asp700I 750 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
980 GAATGGGTTC
AvaI 1640 CCCGGG C^[CT]CG[GA]G
4062 CTCGAG
BbeI 149 GGCGCC GGCGC^C
222 GGCGCC
BbsI 2676 GAAGAC GAAGAC(2/6)
3779 GAAGAC
BciVI 3222 GGATAC GTATCC(6/5)
3455 GTATCC
BpuAI 2676 GAAGAC GAAGAC(2/6)
3779 GAAGAC
BsaI 3387 GAGACC GGTCTC(1/5)
3579 GGTCTC
BsgI 1696 GTGCAG GTGCAG(16/14)
2931 GTGCAG
BsoBI 1640 CCCGGG C^[CT]CG[GA]G
4062 CTCGAG
BsrBI 2229 CCGCTC CCGCTC(-3/-3)
185 GAGCGG
BsrDI 3903 CATTGC GCAATG(2/0)
4006 GCAATG
BstAPI 155 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4311 GCAGCCACTGC
EarI 3207 GAAGAG CTCTTC(1/4)
3216 GAAGAG
HindIII 753 AAGCTT A^AGCTT
1679 AAGCTT
KasI 149 GGCGCC G^GCGCC
222 GGCGCC
Ksp632I 3207 GAAGAG CTCTTC(1/4)
3216 GAAGAG
MslI 1130 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4322 CATCCCAGTG
NarI 149 GGCGCC GG^CGCC
222 GGCGCC
SacI 1110 GAGCTC GAGCT^C
2631 GAGCTC
SexAI 1577 ACCTGGT A^CC[AT]GGT
1691 ACCTGGT
SfoI 149 GGCGCC GGC^GCC
222 GGCGCC
StuI 167 AGGCCT AGG^CCT
1884 AGGCCT
XmnI 750 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
980 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII