################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccIII AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AvrII BamHI BcgI BfrI BglII BlnI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FseI HpaI KspI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 2439 GGTACC G^GTACC AccI 4122 GTAGAC GT^[AC][GT]AC AclI 2407 AACGTT AA^CGTT Alw44I 4350 GTGCAC G^TGCAC ApaLI 4350 GTGCAC G^TGCAC Asp718I 2439 GGTACC G^GTACC AspI 3603 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4003 TGCGCA TGC^GCA BbrPI 595 CACGTG CAC^GTG BclI 935 TGATCA T^GATCA BlpI 2664 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2664 GCTGAGC GC^T[ACGT]AGC BsaBI 1369 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 3062 GAATGC GAATGC(1/-1) BsrFI 23 GCCGGC [GA]^CCGG[CT] BstXI 4159 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2664 GCTGAGC GC^T[ACGT]AGC Cfr10I 23 GCCGGC [GA]^CCGG[CT] DraI 962 TTTAAA TTT^AAA DraIII 3612 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3045 GACTTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 935 TGATCA T^GATCA FspI 4003 TGCGCA TGC^GCA KpnI 2439 GGTACC GGTAC^C MamI 1369 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 23 GCCGGC GCC^GGC NcoI 1361 CCATGG C^CATGG NgoMIV 23 GCCGGC G^CCGGC NspI 3976 ACATGC [GA]CATG^[CT] PaeR7I 4062 CTCGAG C^TCGAG PflFI 3603 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 595 CACGTG CAC^GTG PmlI 595 CACGTG CAC^GTG Psp1406I 2407 AACGTT AA^CGTT ScaI 1290 AGTACT AGT^ACT SmaI 1640 CCCGGG CCC^GGG Tth111I 3603 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 4062 CTCGAG C^TCGAG XmaCI 1640 CCCGGG C^CCGGG XmaI 1640 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 596 ACGTGT A^C[GA][CT]GT 4243 ACGTGT Asp700I 750 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 980 GAATGGGTTC AvaI 1640 CCCGGG C^[CT]CG[GA]G 4062 CTCGAG BbeI 149 GGCGCC GGCGC^C 222 GGCGCC BbsI 2676 GAAGAC GAAGAC(2/6) 3779 GAAGAC BciVI 3222 GGATAC GTATCC(6/5) 3455 GTATCC BpuAI 2676 GAAGAC GAAGAC(2/6) 3779 GAAGAC BsaI 3387 GAGACC GGTCTC(1/5) 3579 GGTCTC BsgI 1696 GTGCAG GTGCAG(16/14) 2931 GTGCAG BsoBI 1640 CCCGGG C^[CT]CG[GA]G 4062 CTCGAG BsrBI 2229 CCGCTC CCGCTC(-3/-3) 185 GAGCGG BsrDI 3903 CATTGC GCAATG(2/0) 4006 GCAATG BstAPI 155 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4311 GCAGCCACTGC EarI 3207 GAAGAG CTCTTC(1/4) 3216 GAAGAG HindIII 753 AAGCTT A^AGCTT 1679 AAGCTT KasI 149 GGCGCC G^GCGCC 222 GGCGCC Ksp632I 3207 GAAGAG CTCTTC(1/4) 3216 GAAGAG MslI 1130 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4322 CATCCCAGTG NarI 149 GGCGCC GG^CGCC 222 GGCGCC SacI 1110 GAGCTC GAGCT^C 2631 GAGCTC SexAI 1577 ACCTGGT A^CC[AT]GGT 1691 ACCTGGT SfoI 149 GGCGCC GGC^GCC 222 GGCGCC StuI 167 AGGCCT AGG^CCT 1884 AGGCCT XmnI 750 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 980 GAATGGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII