##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbrPI BclI
BfrI BlnI BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 116 GACGTC GACGT^C
AcsI 2632 GAATTT [GA]^AATT[CT]
ApoI 2632 GAATTT [GA]^AATT[CT]
Asp700I 3649 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 204 GGCGCC GGCGC^C
BcgI 1017 CGACCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4128 GGATAC GTATCC(6/5)
BglII 364 AGATCT A^GATCT
BsaAI 546 TACGTG [CT]AC^GT[GA]
BsaWI 42 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1530 TGTACA T^GTACA
BsrBI 3191 GAGCGG CCGCTC(-3/-3)
BsrDI 691 CATTGC GCAATG(2/0)
BsrGI 1530 TGTACA T^GTACA
BssHII 2487 GCGCGC G^CGCGC
BssSI 2897 CTCGTG CACGAG(-5/-1)
BstEII 1457 GGTGACC G^GT[ACGT]ACC
BstPI 1457 GGTGACC G^GT[ACGT]ACC
DraIII 1244 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3014 GACTATGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 948 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1457 GGTGACC G^GT[ACGT]ACC
HincII 969 GTCAAC GT[CT]^[GA]AC
HindII 969 GTCAAC GT[CT]^[GA]AC
KasI 204 GGCGCC G^GCGCC
NarI 204 GGCGCC GG^CGCC
NheI 1714 GCTAGC G^CTAGC
PaeR7I 3068 CTCGAG C^TCGAG
PshAI 3262 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 204 GGCGCC GGC^GCC
SmaI 3559 CCCGGG CCC^GGG
SphI 2491 GCATGC GCATG^C
SspBI 1530 TGTACA T^GTACA
SspI 2284 AATATT AAT^ATT
XhoI 3068 CTCGAG C^TCGAG
XmaCI 3559 CCCGGG C^CCGGG
XmaI 3559 CCCGGG C^CCGGG
XmnI 3649 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 1848 CGCG CG^CG
2488 CGCG
AocI 1586 CCTCAGG CC^T[ACGT]AGG
3590 CCTGAGG
Aor51HI 888 AGCGCT AGC^GCT
1891 AGCGCT
BanI 204 GGCGCC G^G[CT][GA]CC
3750 GGCACC
BbsI 332 GTCTTC GAAGAC(2/6)
1154 GTCTTC
BglI 2470 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2783 GCCTACTGGGC
BpuAI 332 GTCTTC GAAGAC(2/6)
1154 GTCTTC
BstAPI 1858 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1921 GCAGTTCCTGC
BstUI 1848 CGCG CG^CG
2488 CGCG
BstXI 285 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3093 CCATAACCGTGG
Bsu36I 1586 CCTCAGG CC^T[ACGT]AGG
3590 CCTGAGG
Eco47III 888 AGCGCT AGC^GCT
1891 AGCGCT
Eco81I 1586 CCTCAGG CC^T[ACGT]AGG
3590 CCTGAGG
HindIII 1206 AAGCTT A^AGCTT
2217 AAGCTT
MseI 225 TTAA T^TAA
1803 TTAA
MvnI 1848 CGCG CG^CG
2488 CGCG
NaeI 984 GCCGGC GCC^GGC
3063 GCCGGC
NgoMIV 984 GCCGGC G^CCGGC
3063 GCCGGC
SexAI 1398 ACCAGGT A^CC[AT]GGT
3428 ACCTGGT
SpeI 3445 ACTAGT A^CTAGT
3889 ACTAGT
Tru9I 225 TTAA T^TAA
1803 TTAA
XcmI 578 CCAAAACGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
834 CCAGGGTGGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BamHI BanII BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII