##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI BamHI
BbeI BbrPI BfrI BsaAI BseAI
BsiWI BsmBI BspEI Bst1107I BstAPI
BstZ17I CpoI DraI EcoRV EcoT22I
FseI HincII HindII HpaI KasI
KspI MfeI MluI MroI MunI
NarI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfoI
SgrAI SnaBI SpeI SphI SwaI
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1967 GACGTC GACGT^C
AccI 3544 GTAGAC GT^[AC][GT]AC
AhdI 4381 GACTAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 3943 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4381 GACTAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 623 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2541 TGCGCA TGC^GCA
AvrII 4406 CCTAGG C^CTAGG
BcgI 3020 GCAAACGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 493 TGATCA T^GATCA
BlnI 4406 CCTAGG C^CTAGG
BlpI 2357 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2357 GCTGAGC GC^T[ACGT]AGC
BsaBI 3006 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1320 ACCGGA [AT]^CCGG[AT]
BsiEI 2697 CGGCCG CG[GA][CT]^CG
BsmI 1339 GAATGC GAATGC(1/-1)
Bsp1407I 2505 TGTACA T^GTACA
BspDI 653 ATCGAT AT^CGAT
BspHI 1140 TCATGA T^CATGA
BsrDI 2764 CATTGC GCAATG(2/0)
BsrGI 2505 TGTACA T^GTACA
BssHII 219 GCGCGC G^CGCGC
BstBI 2486 TTCGAA TT^CGAA
BstEII 4044 GGTCACC G^GT[ACGT]ACC
BstPI 4044 GGTCACC G^GT[ACGT]ACC
CelII 2357 GCTGAGC GC^T[ACGT]AGC
ClaI 653 ATCGAT AT^CGAT
DrdI 4470 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2697 CGGCCG C^GGCCG
Eam1105I 4381 GACTAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2697 CGGCCG C^GGCCG
Eco52I 2697 CGGCCG C^GGCCG
EcoO65I 4044 GGTCACC G^GT[ACGT]ACC
EcoRI 4287 GAATTC G^AATTC
FbaI 493 TGATCA T^GATCA
FspI 2541 TGCGCA TGC^GCA
HindIII 3284 AAGCTT A^AGCTT
MamI 3006 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1380 GCCGGC GCC^GGC
NdeI 948 CATATG CA^TATG
NgoMIV 1380 GCCGGC G^CCGGC
NspV 2486 TTCGAA TT^CGAA
PflFI 623 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3459 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1140 TCATGA T^CATGA
SapI 1930 GCTCTTC GCTCTTC(1/4)
SfiI 1379 GGCCGGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2486 TTCGAA TT^CGAA
SspBI 2505 TGTACA T^GTACA
SspI 974 AATATT AAT^ATT
Tth111I 623 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3943 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 564 ACATGT A^C[GA][CT]GT
3424 ACATGT
AocI 2887 CCTCAGG CC^T[ACGT]AGG
3937 CCTCAGG
Aor51HI 258 AGCGCT AGC^GCT
1924 AGCGCT
BalI 1980 TGGCCA TGG^CCA
4156 TGGCCA
BbvCI 3702 CCTCAGC CCTCAGC(-5/-2)
2468 GCTGAGG
BspLU11I 564 ACATGT A^CATGT
3424 ACATGT
BspMI 369 GCAGGT ACCTGC(4/8)
2038 GCAGGT
BsrBI 364 GAGCGG CCGCTC(-3/-3)
779 GAGCGG
BssSI 2066 CTCGTG CACGAG(-5/-1)
4128 CTCGTG
Bsu36I 2887 CCTCAGG CC^T[ACGT]AGG
3937 CCTCAGG
DraIII 599 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
3443 CACCAAGTG
Eco47III 258 AGCGCT AGC^GCT
1924 AGCGCT
Eco81I 2887 CCTCAGG CC^T[ACGT]AGG
3937 CCTCAGG
MluNI 1980 TGGCCA TGG^CCA
4156 TGGCCA
MscI 1980 TGGCCA TGG^CCA
4156 TGGCCA
MseI 2279 TTAA T^TAA
2285 TTAA
NheI 412 GCTAGC G^CTAGC
1921 GCTAGC
SacI 995 GAGCTC GAGCT^C
3999 GAGCTC
Tru9I 2279 TTAA T^TAA
2285 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI Asp718I AspHI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmaI SmlI Sse8387I
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI