################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI BamHI BbeI BbrPI BfrI BsaAI BseAI BsiWI BsmBI BspEI Bst1107I BstAPI BstZ17I CpoI DraI EcoRV EcoT22I FseI HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NarI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfoI SgrAI SnaBI SpeI SphI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1967 GACGTC GACGT^C AccI 3544 GTAGAC GT^[AC][GT]AC AhdI 4381 GACTAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 3943 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4381 GACTAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 623 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2541 TGCGCA TGC^GCA AvrII 4406 CCTAGG C^CTAGG BcgI 3020 GCAAACGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 493 TGATCA T^GATCA BlnI 4406 CCTAGG C^CTAGG BlpI 2357 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2357 GCTGAGC GC^T[ACGT]AGC BsaBI 3006 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1320 ACCGGA [AT]^CCGG[AT] BsiEI 2697 CGGCCG CG[GA][CT]^CG BsmI 1339 GAATGC GAATGC(1/-1) Bsp1407I 2505 TGTACA T^GTACA BspDI 653 ATCGAT AT^CGAT BspHI 1140 TCATGA T^CATGA BsrDI 2764 CATTGC GCAATG(2/0) BsrGI 2505 TGTACA T^GTACA BssHII 219 GCGCGC G^CGCGC BstBI 2486 TTCGAA TT^CGAA BstEII 4044 GGTCACC G^GT[ACGT]ACC BstPI 4044 GGTCACC G^GT[ACGT]ACC CelII 2357 GCTGAGC GC^T[ACGT]AGC ClaI 653 ATCGAT AT^CGAT DrdI 4470 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2697 CGGCCG C^GGCCG Eam1105I 4381 GACTAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2697 CGGCCG C^GGCCG Eco52I 2697 CGGCCG C^GGCCG EcoO65I 4044 GGTCACC G^GT[ACGT]ACC EcoRI 4287 GAATTC G^AATTC FbaI 493 TGATCA T^GATCA FspI 2541 TGCGCA TGC^GCA HindIII 3284 AAGCTT A^AGCTT MamI 3006 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1380 GCCGGC GCC^GGC NdeI 948 CATATG CA^TATG NgoMIV 1380 GCCGGC G^CCGGC NspV 2486 TTCGAA TT^CGAA PflFI 623 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3459 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1140 TCATGA T^CATGA SapI 1930 GCTCTTC GCTCTTC(1/4) SfiI 1379 GGCCGGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 2486 TTCGAA TT^CGAA SspBI 2505 TGTACA T^GTACA SspI 974 AATATT AAT^ATT Tth111I 623 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3943 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 564 ACATGT A^C[GA][CT]GT 3424 ACATGT AocI 2887 CCTCAGG CC^T[ACGT]AGG 3937 CCTCAGG Aor51HI 258 AGCGCT AGC^GCT 1924 AGCGCT BalI 1980 TGGCCA TGG^CCA 4156 TGGCCA BbvCI 3702 CCTCAGC CCTCAGC(-5/-2) 2468 GCTGAGG BspLU11I 564 ACATGT A^CATGT 3424 ACATGT BspMI 369 GCAGGT ACCTGC(4/8) 2038 GCAGGT BsrBI 364 GAGCGG CCGCTC(-3/-3) 779 GAGCGG BssSI 2066 CTCGTG CACGAG(-5/-1) 4128 CTCGTG Bsu36I 2887 CCTCAGG CC^T[ACGT]AGG 3937 CCTCAGG DraIII 599 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 3443 CACCAAGTG Eco47III 258 AGCGCT AGC^GCT 1924 AGCGCT Eco81I 2887 CCTCAGG CC^T[ACGT]AGG 3937 CCTCAGG MluNI 1980 TGGCCA TGG^CCA 4156 TGGCCA MscI 1980 TGGCCA TGG^CCA 4156 TGGCCA MseI 2279 TTAA T^TAA 2285 TTAA NheI 412 GCTAGC G^CTAGC 1921 GCTAGC SacI 995 GAGCTC GAGCT^C 3999 GAGCTC Tru9I 2279 TTAA T^TAA 2285 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### Acc65I AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp718I AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmaI SmlI Sse8387I StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI