##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflII AflIII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BclI
BfrI BglII BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI Bst1107I BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoT22I FbaI FseI FspI
HindIII KpnI KspI MluI NaeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfiI SgrAI SnaBI
SphI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 884 GACGTC GACGT^C
AccIII 1221 TCCGGA T^CCGGA
AcsI 2867 GAATTC [GA]^AATT[CT]
ApoI 2867 GAATTC [GA]^AATT[CT]
Asp700I 174 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 137 TGGCCA TGG^CCA
BbeI 1452 GGCGCC GGCGC^C
BbrPI 3416 CACGTG CAC^GTG
BciVI 4193 GGATAC GTATCC(6/5)
BglI 3914 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4388 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4388 GCTCAGC GC^T[ACGT]AGC
BsaBI 1337 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1221 TCCGGA T^CCGGA
BsmBI 672 CGTCTC CGTCTC(1/5)
BsmI 3264 GAATGC GAATGC(1/-1)
BspEI 1221 TCCGGA T^CCGGA
BssHII 3501 GCGCGC G^CGCGC
BssSI 1239 CTCGTG CACGAG(-5/-1)
BstAPI 525 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 578 TTCGAA TT^CGAA
CelII 4388 GCTCAGC GC^T[ACGT]AGC
DrdI 2368 GACACTAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1829 CGGCCG C^GGCCG
EclXI 1829 CGGCCG C^GGCCG
Eco52I 1829 CGGCCG C^GGCCG
EcoRI 2867 GAATTC G^AATTC
EcoRV 3136 GATATC GAT^ATC
HpaI 2763 GTTAAC GTT^AAC
KasI 1452 GGCGCC G^GCGCC
MamI 1337 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 312 CAATTG C^AATTG
MluNI 137 TGGCCA TGG^CCA
MroI 1221 TCCGGA T^CCGGA
MscI 137 TGGCCA TGG^CCA
MunI 312 CAATTG C^AATTG
NarI 1452 GGCGCC GG^CGCC
NdeI 1134 CATATG CA^TATG
NspV 578 TTCGAA TT^CGAA
PflMI 2927 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3416 CACGTG CAC^GTG
PmlI 3416 CACGTG CAC^GTG
SfoI 1452 GGCGCC GGC^GCC
SfuI 578 TTCGAA TT^CGAA
SpeI 2371 ACTAGT A^CTAGT
Van91I 2927 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 174 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AgeI 245 ACCGGT A^CCGGT
2341 ACCGGT
AvrII 1265 CCTAGG C^CTAGG
2283 CCTAGG
BbsI 4512 GAAGAC GAAGAC(2/6)
2237 GTCTTC
BcgI 1956 CGAACCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1571 GCAGGTGCTTCG
BlnI 1265 CCTAGG C^CTAGG
2283 CCTAGG
BpuAI 4512 GAAGAC GAAGAC(2/6)
2237 GTCTTC
BsaAI 2231 TACGTG [CT]AC^GT[GA]
3416 CACGTG
BsiEI 1829 CGGCCG CG[GA][CT]^CG
3599 CGACCG
HgaI 1539 GCGTC GACGC(5/10)
3443 GCGTC
NcoI 3107 CCATGG C^CATGG
3574 CCATGG
PinAI 245 ACCGGT A^CCGGT
2341 ACCGGT
SapI 3184 GAAGAGC GCTCTTC(1/4)
4173 GAAGAGC
SexAI 824 ACCTGGT A^CC[AT]GGT
3399 ACCTGGT
SfaNI 656 GCATC GCATC(5/9)
4352 GCATC
TfiI 345 GAATC G^A[AT]TC
665 GAATC
XcmI 2194 CCAGCCCTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4535 CCAGAGCTGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BamHI
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfcI SmaI SmlI
Sse8387I StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmaCI XmaI