##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccI AccIII AflII AflIII
AscI AviII AvrII BamHI BbrPI
BclI BfrI BglII BlnI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I ClaI DrdI EcoRI EcoRV
EcoT22I FbaI FseI FspI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PmaCI PmeI PmlI
PvuI RcaI SacII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI Sse8387I SspBI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 1057 AACGTT AA^CGTT
AhdI 879 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1292 GTGCAC G^TGCAC
AocI 3205 CCTCAGG CC^T[ACGT]AGG
ApaI 332 GGGCCC GGGCC^C
ApaLI 1292 GTGCAC G^TGCAC
AseI 3418 ATTAAT AT^TAAT
AsnI 3418 ATTAAT AT^TAAT
Asp700I 4053 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 879 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 455 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1240 TGGCCA TGG^CCA
BbeI 2178 GGCGCC GGCGC^C
BcgI 3012 GCATCTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1559 GTATCC GTATCC(6/5)
BsaI 412 GGTCTC GGTCTC(1/5)
BsmBI 1651 CGTCTC CGTCTC(1/5)
BsmI 1202 GAATGC GAATGC(1/-1)
BsrBI 3031 CCGCTC CCGCTC(-3/-3)
BssHII 2869 GCGCGC G^CGCGC
Bsu36I 3205 CCTCAGG CC^T[ACGT]AGG
CpoI 3281 CGGACCG CG^G[AT]CCG
DraIII 1295 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 879 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3205 CCTCAGG CC^T[ACGT]AGG
HgaI 35 GACGC GACGC(5/10)
HpaI 681 GTTAAC GTT^AAC
KasI 2178 GGCGCC G^GCGCC
MluNI 1240 TGGCCA TGG^CCA
MscI 1240 TGGCCA TGG^CCA
NaeI 441 GCCGGC GCC^GGC
NarI 2178 GGCGCC GG^CGCC
NgoMIV 441 GCCGGC G^CCGGC
NspI 3806 GCATGC [GA]CATG^[CT]
PaeR7I 2904 CTCGAG C^TCGAG
PflFI 455 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3330 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3418 ATTAAT AT^TAAT
Psp1406I 1057 AACGTT AA^CGTT
RsrII 3281 CGGACCG CG^G[AT]CCG
SfoI 2178 GGCGCC GGC^GCC
SgrAI 1825 CACCGGTG C[GA]^CCGG[CT]G
SphI 3806 GCATGC GCATG^C
StuI 2222 AGGCCT AGG^CCT
SwaI 3402 ATTTAAAT ATTT^AAAT
Tth111I 455 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2904 CTCGAG C^TCGAG
XmnI 4053 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1691 GGTACC G^GTACC
2194 GGTACC
AcyI 35 GACGCC G[GA]^CG[CT]C
2178 GGCGCC
AgeI 880 ACCGGT A^CCGGT
1826 ACCGGT
Aor51HI 1565 AGCGCT AGC^GCT
2888 AGCGCT
Asp718I 1691 GGTACC G^GTACC
2194 GGTACC
BbsI 651 GAAGAC GAAGAC(2/6)
876 GAAGAC
BbvCI 1738 CCTCAGC CCTCAGC(-5/-2)
1791 CCTCAGC
BpuAI 651 GAAGAC GAAGAC(2/6)
876 GAAGAC
BsaAI 1333 TACGTG [CT]AC^GT[GA]
1556 CACGTA
BsaHI 35 GACGCC G[GA]^CG[CT]C
2178 GGCGCC
BsiEI 3064 CGGCCG CG[GA][CT]^CG
3136 CGGCCG
BspMI 2197 ACCTGC ACCTGC(4/8)
2761 ACCTGC
BssSI 2114 CACGAG CACGAG(-5/-1)
2936 CACGAG
BstAPI 2309 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3715 GCAGGGCCTGC
BstEII 3182 GGTTACC G^GT[ACGT]ACC
3937 GGTCACC
BstF5I 1577 CATCC GGATG(2/0)
1513 GGATG
BstPI 3182 GGTTACC G^GT[ACGT]ACC
3937 GGTCACC
EagI 3064 CGGCCG C^GGCCG
3136 CGGCCG
EclXI 3064 CGGCCG C^GGCCG
3136 CGGCCG
Eco47III 1565 AGCGCT AGC^GCT
2888 AGCGCT
Eco52I 3064 CGGCCG C^GGCCG
3136 CGGCCG
EcoNI 2246 CCTATCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3677 CCTTTTAAAGG
EcoO65I 3182 GGTTACC G^GT[ACGT]ACC
3937 GGTCACC
FokI 1577 CATCC GGATG(9/13)
1513 GGATG
Hin1I 35 GACGCC G[GA]^CG[CT]C
2178 GGCGCC
HindIII 3373 AAGCTT A^AGCTT
3462 AAGCTT
KpnI 1691 GGTACC GGTAC^C
2194 GGTACC
PinAI 880 ACCGGT A^CCGGT
1826 ACCGGT
SacI 14 GAGCTC GAGCT^C
370 GAGCTC
SspI 1411 AATATT AAT^ATT
3628 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI