##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AcyI AgeI AhdI Aor51HI
AscI AspEI AspI AvaI BbeI
BcgI BciVI BlpI Bpu1102I BsaBI
BsaHI BsiEI BsiWI BsmBI BsoBI
BspDI BspHI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FseI HaeII HgaI
Hin1I HpaI KasI KspI MamI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3248 GGTACC G^GTACC
AccI 477 GTAGAC GT^[AC][GT]AC
AccIII 130 TCCGGA T^CCGGA
AclI 1178 AACGTT AA^CGTT
AflII 424 CTTAAG C^TTAAG
AocI 4142 CCTCAGG CC^T[ACGT]AGG
ApaI 4138 GGGCCC GGGCC^C
Asp718I 3248 GGTACC G^GTACC
AviII 4604 TGCGCA TGC^GCA
AvrII 2976 CCTAGG C^CTAGG
BamHI 3426 GGATCC G^GATCC
BbrPI 3364 CACGTG CAC^GTG
BbvCI 158 CCTCAGC CCTCAGC(-5/-2)
BcnI 36 CCCGG CC^[GC]GG
BfrI 424 CTTAAG C^TTAAG
BglII 655 AGATCT A^GATCT
BlnI 2976 CCTAGG C^CTAGG
BsaAI 3364 CACGTG [CT]AC^GT[GA]
BseAI 130 TCCGGA T^CCGGA
BseRI 156 CTCCTC GAGGAG(10/8)
Bsp1407I 4467 TGTACA T^GTACA
BspEI 130 TCCGGA T^CCGGA
BsrBI 1756 CCGCTC CCGCTC(-3/-3)
BsrGI 4467 TGTACA T^GTACA
BssSI 2356 CACGAG CACGAG(-5/-1)
BstAPI 4710 GCAACAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 81 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4142 CCTCAGG CC^T[ACGT]AGG
CfoI 4605 GCGC GCG^C
DraIII 4308 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4142 CCTCAGG CC^T[ACGT]AGG
EcoT22I 4598 ATGCAT ATGCA^T
FspI 4604 TGCGCA TGC^GCA
HhaI 4605 GCGC GCG^C
HinP1I 4605 GCGC G^CGC
KpnI 3248 GGTACC GGTAC^C
MfeI 1063 CAATTG C^AATTG
MluI 1187 ACGCGT A^CGCGT
MroI 130 TCCGGA T^CCGGA
MspA1I 3563 CAGCGG C[AC]G^C[GT]G
MunI 1063 CAATTG C^AATTG
NciI 36 CCCGG CC^[GC]GG
NdeI 4405 CATATG CA^TATG
NsiI 4598 ATGCAT ATGCA^T
PmaCI 3364 CACGTG CAC^GTG
PmlI 3364 CACGTG CAC^GTG
Psp1406I 1178 AACGTT AA^CGTT
PstI 234 CTGCAG CTGCA^G
SexAI 204 ACCTGGT A^CC[AT]GGT
SspBI 4467 TGTACA T^GTACA
StuI 1659 AGGCCT AGG^CCT
XbaI 868 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 107 CGCG CG^CG
1188 CGCG
Alw44I 2297 GTGCAC G^TGCAC
2533 GTGCAC
ApaLI 2297 GTGCAC G^TGCAC
2533 GTGCAC
BanI 3248 GGTACC G^G[CT][GA]CC
4617 GGCACC
BclI 495 TGATCA T^GATCA
736 TGATCA
BglI 34 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3330 GCCAGATAGGC
BsaI 3774 GAGACC GGTCTC(1/5)
208 GGTCTC
BsaWI 130 TCCGGA [AT]^CCGG[AT]
2304 ACCGGA
BsmI 143 GAATGC GAATGC(1/-1)
1201 GCATTC
BspLU11I 897 ACATGT A^CATGT
3397 ACATGT
BstUI 107 CGCG CG^CG
1188 CGCG
DraII 4137 GGGGCCC [GA]G^G[ACGT]CC[CT]
4138 GGGCCCT
DsaI 81 CCATGG C^C[GA][CT]GG
472 CCATGG
EcoO109I 4137 GGGGCCC [GA]G^G[ACGT]CC[CT]
4138 GGGCCCT
FauI 3979 CCCGC CCCGC(4/6)
3565 GCGGG
FbaI 495 TGATCA T^GATCA
736 TGATCA
MvnI 107 CGCG CG^CG
1188 CGCG
NcoI 81 CCATGG C^CATGG
472 CCATGG
PflMI 21 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4646 CCATACCTTGG
SacI 239 GAGCTC GAGCT^C
3962 GAGCTC
TaqI 744 TCGA T^CGA
4261 TCGA
TthHB8I 744 TCGA T^CGA
4261 TCGA
Van91I 21 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4646 CCATACCTTGG
XcmI 3834 CCAGGCTTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4680 CCATGTGAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EaeI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI