##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspI AvrII BbeI BbvCI
BcgI BciVI BclI BlnI BsaBI
BsaHI BsiWI BspDI BsrDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
Eco47III EcoNI EcoT22I FbaI FseI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 2573 CGCG CG^CG
AccIII 3287 TCCGGA T^CCGGA
AflII 2377 CTTAAG C^TTAAG
AhdI 2387 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3651 ATTAAT AT^TAAT
AsnI 3651 ATTAAT AT^TAAT
AspEI 2387 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 636 TGCGCA TGC^GCA
BamHI 2484 GGATCC G^GATCC
BbrPI 3310 CACGTG CAC^GTG
BfrI 2377 CTTAAG C^TTAAG
BglI 2311 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1096 AGATCT A^GATCT
BlpI 4110 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4110 GCTCAGC GC^T[ACGT]AGC
BsaI 1056 GGTCTC GGTCTC(1/5)
BsaWI 3287 TCCGGA [AT]^CCGG[AT]
BseAI 3287 TCCGGA T^CCGGA
BsiEI 1782 CGGCCG CG[GA][CT]^CG
BsmBI 2546 CGTCTC CGTCTC(1/5)
BspEI 3287 TCCGGA T^CCGGA
BspHI 2850 TCATGA T^CATGA
BspMI 2155 GCAGGT ACCTGC(4/8)
BssSI 3899 CTCGTG CACGAG(-5/-1)
BstUI 2573 CGCG CG^CG
CelII 4110 GCTCAGC GC^T[ACGT]AGC
DraIII 3578 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1309 GACTGTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1782 CGGCCG C^GGCCG
Eam1105I 2387 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3522 GAAGAG CTCTTC(1/4)
EclXI 1782 CGGCCG C^GGCCG
Eco52I 1782 CGGCCG C^GGCCG
EcoRI 4415 GAATTC G^AATTC
EcoRV 3204 GATATC GAT^ATC
FspI 636 TGCGCA TGC^GCA
HaeII 1293 AGCGCC [GA]GCGC^[CT]
HindIII 857 AAGCTT A^AGCTT
Ksp632I 3522 GAAGAG CTCTTC(1/4)
MroI 3287 TCCGGA T^CCGGA
MvnI 2573 CGCG CG^CG
NaeI 1780 GCCGGC GCC^GGC
NdeI 2248 CATATG CA^TATG
NgoMIV 1780 GCCGGC G^CCGGC
PaeR7I 1110 CTCGAG C^TCGAG
PmaCI 3310 CACGTG CAC^GTG
PmlI 3310 CACGTG CAC^GTG
PshBI 3651 ATTAAT AT^TAAT
RcaI 2850 TCATGA T^CATGA
ScaI 4583 AGTACT AGT^ACT
SmaI 1525 CCCGGG CCC^GGG
SphI 513 GCATGC GCATG^C
StuI 3511 AGGCCT AGG^CCT
XbaI 2661 TCTAGA T^CTAGA
XhoI 1110 CTCGAG C^TCGAG
XmaCI 1525 CCCGGG C^CCGGG
XmaI 1525 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 1110 CTCGAG C^[CT]CG[GA]G
1525 CCCGGG
BalI 964 TGGCCA TGG^CCA
1631 TGGCCA
BbsI 605 GAAGAC GAAGAC(2/6)
3414 GAAGAC
BcnI 1525 CCCGG CC^[GC]GG
1526 CCGGG
BpuAI 605 GAAGAC GAAGAC(2/6)
3414 GAAGAC
BsoBI 1110 CTCGAG C^[CT]CG[GA]G
1525 CCCGGG
BspLU11I 2675 ACATGT A^CATGT
3822 ACATGT
BsrBI 43 CCGCTC CCGCTC(-3/-3)
145 CCGCTC
BsrFI 1780 GCCGGC [GA]^CCGG[CT]
4388 GCCGGT
BstEII 3878 GGTTACC G^GT[ACGT]ACC
4465 GGTCACC
BstPI 3878 GGTTACC G^GT[ACGT]ACC
4465 GGTCACC
Cfr10I 1780 GCCGGC [GA]^CCGG[CT]
4388 GCCGGT
DsaI 392 CCACGG C^C[GA][CT]GG
4061 CCACGG
EcoO65I 3878 GGTTACC G^GT[ACGT]ACC
4465 GGTCACC
MluNI 964 TGGCCA TGG^CCA
1631 TGGCCA
MscI 964 TGGCCA TGG^CCA
1631 TGGCCA
MspA1I 290 CAGCTG C[AC]G^C[GT]G
594 CAGCTG
NciI 1525 CCCGG CC^[GC]GG
1526 CCGGG
PvuII 290 CAGCTG CAG^CTG
594 CAGCTG
SacI 2213 GAGCTC GAGCT^C
4108 GAGCTC
TaqI 270 TCGA T^CGA
1111 TCGA
TthHB8I 270 TCGA T^CGA
1111 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BanI BanII
BbvI BfaI BmyI BpmI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmnI