##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI Aor51HI ApoI
AscI Asp718I AspI AvaI AviII
AvrII BamHI BbrPI BcgI BciVI
BfrI BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsoBI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I CpoI DraIII
EagI EclXI Eco47III Eco52I EcoRI
EcoT22I FseI FspI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI SspI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 3290 GTCTAC GT^[AC][GT]AC
AflIII 3144 ACATGT A^C[GA][CT]GT
Alw44I 1424 GTGCAC G^TGCAC
ApaLI 1424 GTGCAC G^TGCAC
AseI 294 ATTAAT AT^TAAT
AsnI 294 ATTAAT AT^TAAT
Asp700I 1106 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 988 GGCGCC GGCGC^C
BglI 1857 GCCAGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1281 AGATCT A^GATCT
BsaWI 278 TCCGGT [AT]^CCGG[AT]
BsmI 2480 GCATTC GAATGC(1/-1)
Bsp1407I 2247 TGTACA T^GTACA
BspDI 1770 ATCGAT AT^CGAT
BspHI 758 TCATGA T^CATGA
BspLU11I 3144 ACATGT A^CATGT
BsrDI 3075 CATTGC GCAATG(2/0)
BsrGI 2247 TGTACA T^GTACA
BssSI 703 CACGAG CACGAG(-5/-1)
BstAPI 2181 GCATATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1770 ATCGAT AT^CGAT
DrdI 2294 GACACTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2489 GATATC GAT^ATC
HincII 679 GTTAAC GT[CT]^[GA]AC
HindII 679 GTTAAC GT[CT]^[GA]AC
HindIII 544 AAGCTT A^AGCTT
HpaI 679 GTTAAC GTT^AAC
KasI 988 GGCGCC G^GCGCC
MaeII 3254 ACGT A^CGT
NarI 988 GGCGCC GG^CGCC
NheI 3384 GCTAGC G^CTAGC
PpuMI 2351 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 351 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 294 ATTAAT AT^TAAT
RcaI 758 TCATGA T^CATGA
SapI 564 GAAGAGC GCTCTTC(1/4)
SbfI 855 CCTGCAGG CCTGCA^GG
SfoI 988 GGCGCC GGC^GCC
SmlI 1365 CTCAAG C^T[CT][GA]AG
SpeI 3286 ACTAGT A^CTAGT
Sse8387I 855 CCTGCAGG CCTGCA^GG
SspBI 2247 TGTACA T^GTACA
StuI 1197 AGGCCT AGG^CCT
SwaI 3530 ATTTAAAT ATTT^AAAT
TfiI 1768 GAATC G^A[AT]TC
XbaI 3344 TCTAGA T^CTAGA
XmnI 1106 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AhdI 328 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACAACCTGTC
AspEI 328 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACAACCTGTC
BbvCI 3218 CCTCAGC CCTCAGC(-5/-2)
1126 GCTGAGG
BclI 117 TGATCA T^GATCA
1551 TGATCA
BlpI 1492 GCTGAGC GC^T[ACGT]AGC
2112 GCTGAGC
Bpu1102I 1492 GCTGAGC GC^T[ACGT]AGC
2112 GCTGAGC
BstYI 176 AGATCC [GA]^GATC[CT]
1281 AGATCT
CelII 1492 GCTGAGC GC^T[ACGT]AGC
2112 GCTGAGC
CfoI 657 GCGC GCG^C
989 GCGC
DsaI 470 CCATGG C^C[GA][CT]GG
614 CCATGG
Eam1105I 328 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
868 GACAACCTGTC
FauI 1210 CCCGC CCCGC(4/6)
1632 CCCGC
FbaI 117 TGATCA T^GATCA
1551 TGATCA
HaeII 656 AGCGCC [GA]GCGC^[CT]
988 GGCGCC
HhaI 657 GCGC GCG^C
989 GCGC
HinP1I 657 GCGC G^CGC
989 GCGC
MflI 176 AGATCC [GA]^GATC[CT]
1281 AGATCT
MslI 188 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
465 CATCCCCATG
NcoI 470 CCATGG C^CATGG
614 CCATGG
NdeI 424 CATATG CA^TATG
2182 CATATG
NspI 3144 ACATGT [GA]CATG^[CT]
3623 GCATGT
PflMI 1975 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3467 CCAGAGACTGG
PvuII 2110 CAGCTG CAG^CTG
3583 CAGCTG
Van91I 1975 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3467 CCAGAGACTGG
XcmI 3017 CCACACAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3322 CCACTACATCTTTGG
XhoII 176 AGATCC [GA]^GATC[CT]
1281 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I