##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AclI AgeI Alw44I ApaLI
AscI AseI AsnI AviII AvrII
BcgI BlnI BlpI Bpu1102I BsaBI
BsiWI BsmBI BsmI BspDI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I CelII ClaI DraI EcoNI
EcoO65I EcoRV FseI FspI HindIII
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII SalI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2321 GGTACC G^GTACC
AccIII 2625 TCCGGA T^CCGGA
AflII 1600 CTTAAG C^TTAAG
AhdI 1507 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3204 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2095 AGCGCT AGC^GCT
ApaI 254 GGGCCC GGGCC^C
Asp718I 2321 GGTACC G^GTACC
AspEI 1507 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 3213 GTATCC GTATCC(6/5)
BclI 2819 TGATCA T^GATCA
BfrI 1600 CTTAAG C^TTAAG
BglII 3128 AGATCT A^GATCT
BseAI 2625 TCCGGA T^CCGGA
Bsp1407I 1049 TGTACA T^GTACA
BspEI 2625 TCCGGA T^CCGGA
BspHI 2765 TCATGA T^CATGA
BspLU11I 1442 ACATGT A^CATGT
BsrBI 191 CCGCTC CCGCTC(-3/-3)
BsrGI 1049 TGTACA T^GTACA
BstBI 788 TTCGAA TT^CGAA
Bsu36I 3204 CCTTAGG CC^T[ACGT]AGG
CpoI 116 CGGTCCG CG^G[AT]CCG
DraIII 3358 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1507 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2095 AGCGCT AGC^GCT
Eco81I 3204 CCTTAGG CC^T[ACGT]AGG
EcoRI 3096 GAATTC G^AATTC
EcoT22I 1763 ATGCAT ATGCA^T
FbaI 2819 TGATCA T^GATCA
HincII 2956 GTCAAC GT[CT]^[GA]AC
HindII 2956 GTCAAC GT[CT]^[GA]AC
KpnI 2321 GGTACC GGTAC^C
KspI 151 CCGCGG CCGC^GG
MroI 2625 TCCGGA T^CCGGA
NsiI 1763 ATGCAT ATGCA^T
NspV 788 TTCGAA TT^CGAA
PflMI 2853 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1824 CTGCAG CTGCA^G
RcaI 2765 TCATGA T^CATGA
RsrII 116 CGGTCCG CG^G[AT]CCG
SacII 151 CCGCGG CCGC^GG
SapI 1867 GAAGAGC GCTCTTC(1/4)
SfuI 788 TTCGAA TT^CGAA
SspBI 1049 TGTACA T^GTACA
StuI 2255 AGGCCT AGG^CCT
Van91I 2853 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2252 CCAAGGCCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1512 GACGTC GACGT^C
3477 GACGTC
AcsI 544 AAATTC [GA]^AATT[CT]
3096 GAATTC
AlwNI 1855 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
2854 CAGGCCCTG
ApoI 544 AAATTC [GA]^AATT[CT]
3096 GAATTC
BalI 1110 TGGCCA TGG^CCA
1283 TGGCCA
BamHI 866 GGATCC G^GATCC
1811 GGATCC
BbrPI 73 CACGTG CAC^GTG
1034 CACGTG
BbsI 2197 GAAGAC GAAGAC(2/6)
3103 GTCTTC
BbvCI 769 CCTCAGC CCTCAGC(-5/-2)
2356 CCTCAGC
BglI 2401 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3308 GCCCTGGCGGC
BpuAI 2197 GAAGAC GAAGAC(2/6)
3103 GTCTTC
BsiEI 129 CGGCCG CG[GA][CT]^CG
200 CGGCCG
BsrFI 127 GCCGGC [GA]^CCGG[CT]
551 GCCGGC
Cfr10I 127 GCCGGC [GA]^CCGG[CT]
551 GCCGGC
DrdI 1368 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2947 GACCGCTGGGTC
EagI 129 CGGCCG C^GGCCG
200 CGGCCG
EclXI 129 CGGCCG C^GGCCG
200 CGGCCG
Eco52I 129 CGGCCG C^GGCCG
200 CGGCCG
HgaI 1879 GACGC GACGC(5/10)
2814 GCGTC
MluNI 1110 TGGCCA TGG^CCA
1283 TGGCCA
MscI 1110 TGGCCA TGG^CCA
1283 TGGCCA
NaeI 127 GCCGGC GCC^GGC
551 GCCGGC
NcoI 272 CCATGG C^CATGG
2471 CCATGG
NgoMIV 127 GCCGGC G^CCGGC
551 GCCGGC
PaeR7I 22 CTCGAG C^TCGAG
1225 CTCGAG
PmaCI 73 CACGTG CAC^GTG
1034 CACGTG
PmlI 73 CACGTG CAC^GTG
1034 CACGTG
PpuMI 241 AGGACCC [GA]G^G[AT]CC[CT]
2347 AGGACCT
SacI 373 GAGCTC GAGCT^C
3426 GAGCTC
SexAI 1522 ACCTGGT A^CC[AT]GGT
2231 ACCAGGT
SmaI 491 CCCGGG CCC^GGG
3187 CCCGGG
XhoI 22 CTCGAG C^TCGAG
1225 CTCGAG
XmaCI 491 CCCGGG C^CCGGG
3187 CCCGGG
XmaI 491 CCCGGG C^CCGGG
3187 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI Asp700I AspHI AspI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BmyI BpmI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmnI