################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AccI AclI AgeI Alw44I ApaLI AscI AseI AsnI AviII AvrII BcgI BlnI BlpI Bpu1102I BsaBI BsiWI BsmBI BsmI BspDI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII ClaI DraI EcoNI EcoO65I EcoRV FseI FspI HindIII HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI PacI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 2321 GGTACC G^GTACC AccIII 2625 TCCGGA T^CCGGA AflII 1600 CTTAAG C^TTAAG AhdI 1507 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3204 CCTTAGG CC^T[ACGT]AGG Aor51HI 2095 AGCGCT AGC^GCT ApaI 254 GGGCCC GGGCC^C Asp718I 2321 GGTACC G^GTACC AspEI 1507 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 3213 GTATCC GTATCC(6/5) BclI 2819 TGATCA T^GATCA BfrI 1600 CTTAAG C^TTAAG BglII 3128 AGATCT A^GATCT BseAI 2625 TCCGGA T^CCGGA Bsp1407I 1049 TGTACA T^GTACA BspEI 2625 TCCGGA T^CCGGA BspHI 2765 TCATGA T^CATGA BspLU11I 1442 ACATGT A^CATGT BsrBI 191 CCGCTC CCGCTC(-3/-3) BsrGI 1049 TGTACA T^GTACA BstBI 788 TTCGAA TT^CGAA Bsu36I 3204 CCTTAGG CC^T[ACGT]AGG CpoI 116 CGGTCCG CG^G[AT]CCG DraIII 3358 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1507 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2095 AGCGCT AGC^GCT Eco81I 3204 CCTTAGG CC^T[ACGT]AGG EcoRI 3096 GAATTC G^AATTC EcoT22I 1763 ATGCAT ATGCA^T FbaI 2819 TGATCA T^GATCA HincII 2956 GTCAAC GT[CT]^[GA]AC HindII 2956 GTCAAC GT[CT]^[GA]AC KpnI 2321 GGTACC GGTAC^C KspI 151 CCGCGG CCGC^GG MroI 2625 TCCGGA T^CCGGA NsiI 1763 ATGCAT ATGCA^T NspV 788 TTCGAA TT^CGAA PflMI 2853 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1824 CTGCAG CTGCA^G RcaI 2765 TCATGA T^CATGA RsrII 116 CGGTCCG CG^G[AT]CCG SacII 151 CCGCGG CCGC^GG SapI 1867 GAAGAGC GCTCTTC(1/4) SfuI 788 TTCGAA TT^CGAA SspBI 1049 TGTACA T^GTACA StuI 2255 AGGCCT AGG^CCT Van91I 2853 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2252 CCAAGGCCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AatII 1512 GACGTC GACGT^C 3477 GACGTC AcsI 544 AAATTC [GA]^AATT[CT] 3096 GAATTC AlwNI 1855 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 2854 CAGGCCCTG ApoI 544 AAATTC [GA]^AATT[CT] 3096 GAATTC BalI 1110 TGGCCA TGG^CCA 1283 TGGCCA BamHI 866 GGATCC G^GATCC 1811 GGATCC BbrPI 73 CACGTG CAC^GTG 1034 CACGTG BbsI 2197 GAAGAC GAAGAC(2/6) 3103 GTCTTC BbvCI 769 CCTCAGC CCTCAGC(-5/-2) 2356 CCTCAGC BglI 2401 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3308 GCCCTGGCGGC BpuAI 2197 GAAGAC GAAGAC(2/6) 3103 GTCTTC BsiEI 129 CGGCCG CG[GA][CT]^CG 200 CGGCCG BsrFI 127 GCCGGC [GA]^CCGG[CT] 551 GCCGGC Cfr10I 127 GCCGGC [GA]^CCGG[CT] 551 GCCGGC DrdI 1368 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2947 GACCGCTGGGTC EagI 129 CGGCCG C^GGCCG 200 CGGCCG EclXI 129 CGGCCG C^GGCCG 200 CGGCCG Eco52I 129 CGGCCG C^GGCCG 200 CGGCCG HgaI 1879 GACGC GACGC(5/10) 2814 GCGTC MluNI 1110 TGGCCA TGG^CCA 1283 TGGCCA MscI 1110 TGGCCA TGG^CCA 1283 TGGCCA NaeI 127 GCCGGC GCC^GGC 551 GCCGGC NcoI 272 CCATGG C^CATGG 2471 CCATGG NgoMIV 127 GCCGGC G^CCGGC 551 GCCGGC PaeR7I 22 CTCGAG C^TCGAG 1225 CTCGAG PmaCI 73 CACGTG CAC^GTG 1034 CACGTG PmlI 73 CACGTG CAC^GTG 1034 CACGTG PpuMI 241 AGGACCC [GA]G^G[AT]CC[CT] 2347 AGGACCT SacI 373 GAGCTC GAGCT^C 3426 GAGCTC SexAI 1522 ACCTGGT A^CC[AT]GGT 2231 ACCAGGT SmaI 491 CCCGGG CCC^GGG 3187 CCCGGG XhoI 22 CTCGAG C^TCGAG 1225 CTCGAG XmaCI 491 CCCGGG C^CCGGG 3187 CCCGGG XmaI 491 CCCGGG C^CCGGG 3187 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI Asp700I AspHI AspI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflFI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmnI