################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI Alw44I ApaI ApaLI AscI Asp718I AviII AvrII BalI BbeI BbrPI BbvCI BcgI BclI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraII EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HpaI KasI KpnI MamI MluI MluNI MroI MscI NaeI NarI NgoMIV NotI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI XcmI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Aor51HI 3141 AGCGCT AGC^GCT AspHI 2182 GAGCTC G[AT]GC[AT]^C AspI 283 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 832 GGATCC G^GATCC BciVI 2614 GGATAC GTATCC(6/5) BglI 3551 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 519 AGATCT A^GATCT BsaAI 580 CACGTA [CT]AC^GT[GA] BsaI 259 GAGACC GGTCTC(1/5) BsiEI 91 CGGCCG CG[GA][CT]^CG BsiHKAI 2182 GAGCTC G[AT]GC[AT]^C BssSI 647 CACGAG CACGAG(-5/-1) BstAPI 1327 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2904 CCACACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 91 CGGCCG C^GGCCG EclXI 91 CGGCCG C^GGCCG Eco47III 3141 AGCGCT AGC^GCT Eco52I 91 CGGCCG C^GGCCG EcoRI 237 GAATTC G^AATTC HgaI 3431 GCGTC GACGC(5/10) KspI 19 CCGCGG CCGC^GG MslI 1412 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 828 CCATGG C^CATGG NruI 44 TCGCGA TCG^CGA PaeR7I 1293 CTCGAG C^TCGAG PflFI 283 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 2182 GAGCTC GAGCT^C SacII 19 CCGCGG CCGC^GG SalI 2960 GTCGAC G^TCGAC SexAI 1666 ACCTGGT A^CC[AT]GGT SphI 2680 GCATGC GCATG^C SspI 188 AATATT AAT^ATT Tth111I 283 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1293 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 2960 GTCGAC GT^[AC][GT]AC 3433 GTCTAC AflII 1465 CTTAAG C^TTAAG 3891 CTTAAG AocI 527 CCTCAGG CC^T[ACGT]AGG 2825 CCTGAGG BfrI 1465 CTTAAG C^TTAAG 3891 CTTAAG BssHII 80 GCGCGC G^CGCGC 82 GCGCGC Bsu36I 527 CCTCAGG CC^T[ACGT]AGG 2825 CCTGAGG DraIII 1743 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG 2696 CACTTTGTG DrdI 3 GACCGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3340 GACAGAAGGGTC EaeI 91 CGGCCG [CT]^GGCC[GA] 2390 CGGCCA Eco81I 527 CCTCAGG CC^T[ACGT]AGG 2825 CCTGAGG HaeII 346 GGCGCT [GA]GCGC^[CT] 3141 AGCGCT HincII 2960 GTCGAC GT[CT]^[GA]AC 3337 GTTGAC HindII 2960 GTCGAC GT[CT]^[GA]AC 3337 GTTGAC NdeI 2199 CATATG CA^TATG 4405 CATATG NheI 2729 GCTAGC G^CTAGC 3028 GCTAGC NspI 1266 GCATGT [GA]CATG^[CT] 2680 GCATGC PflMI 1817 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2650 CCATTTCCTGG PshAI 1463 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3388 GACTCCAGTC PstI 879 CTGCAG CTGCA^G 2625 CTGCAG PvuII 939 CAGCTG CAG^CTG 3631 CAGCTG SapI 2429 GAAGAGC GCTCTTC(1/4) 2494 GAAGAGC SmaI 56 CCCGGG CCC^GGG 158 CCCGGG SwaI 183 ATTTAAAT ATTT^AAAT 402 ATTTAAAT Van91I 1817 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2650 CCATTTCCTGG XbaI 2143 TCTAGA T^CTAGA 3418 TCTAGA XmaCI 56 CCCGGG C^CCGGG 158 CCCGGG XmaI 56 CCCGGG C^CCGGG 158 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspEI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI Eam1105I EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PshBI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI