##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I ApaI ApaLI
AscI Asp718I AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BclI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraII EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KpnI
MamI MluI MluNI MroI MscI
NaeI NarI NgoMIV NotI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RcaI
RsrII SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
XcmI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Aor51HI 3141 AGCGCT AGC^GCT
AspHI 2182 GAGCTC G[AT]GC[AT]^C
AspI 283 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 832 GGATCC G^GATCC
BciVI 2614 GGATAC GTATCC(6/5)
BglI 3551 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 519 AGATCT A^GATCT
BsaAI 580 CACGTA [CT]AC^GT[GA]
BsaI 259 GAGACC GGTCTC(1/5)
BsiEI 91 CGGCCG CG[GA][CT]^CG
BsiHKAI 2182 GAGCTC G[AT]GC[AT]^C
BssSI 647 CACGAG CACGAG(-5/-1)
BstAPI 1327 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2904 CCACACTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 91 CGGCCG C^GGCCG
EclXI 91 CGGCCG C^GGCCG
Eco47III 3141 AGCGCT AGC^GCT
Eco52I 91 CGGCCG C^GGCCG
EcoRI 237 GAATTC G^AATTC
HgaI 3431 GCGTC GACGC(5/10)
KspI 19 CCGCGG CCGC^GG
MslI 1412 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 828 CCATGG C^CATGG
NruI 44 TCGCGA TCG^CGA
PaeR7I 1293 CTCGAG C^TCGAG
PflFI 283 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2182 GAGCTC GAGCT^C
SacII 19 CCGCGG CCGC^GG
SalI 2960 GTCGAC G^TCGAC
SexAI 1666 ACCTGGT A^CC[AT]GGT
SphI 2680 GCATGC GCATG^C
SspI 188 AATATT AAT^ATT
Tth111I 283 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1293 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2960 GTCGAC GT^[AC][GT]AC
3433 GTCTAC
AflII 1465 CTTAAG C^TTAAG
3891 CTTAAG
AocI 527 CCTCAGG CC^T[ACGT]AGG
2825 CCTGAGG
BfrI 1465 CTTAAG C^TTAAG
3891 CTTAAG
BssHII 80 GCGCGC G^CGCGC
82 GCGCGC
Bsu36I 527 CCTCAGG CC^T[ACGT]AGG
2825 CCTGAGG
DraIII 1743 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2696 CACTTTGTG
DrdI 3 GACCGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3340 GACAGAAGGGTC
EaeI 91 CGGCCG [CT]^GGCC[GA]
2390 CGGCCA
Eco81I 527 CCTCAGG CC^T[ACGT]AGG
2825 CCTGAGG
HaeII 346 GGCGCT [GA]GCGC^[CT]
3141 AGCGCT
HincII 2960 GTCGAC GT[CT]^[GA]AC
3337 GTTGAC
HindII 2960 GTCGAC GT[CT]^[GA]AC
3337 GTTGAC
NdeI 2199 CATATG CA^TATG
4405 CATATG
NheI 2729 GCTAGC G^CTAGC
3028 GCTAGC
NspI 1266 GCATGT [GA]CATG^[CT]
2680 GCATGC
PflMI 1817 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2650 CCATTTCCTGG
PshAI 1463 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACTCCAGTC
PstI 879 CTGCAG CTGCA^G
2625 CTGCAG
PvuII 939 CAGCTG CAG^CTG
3631 CAGCTG
SapI 2429 GAAGAGC GCTCTTC(1/4)
2494 GAAGAGC
SmaI 56 CCCGGG CCC^GGG
158 CCCGGG
SwaI 183 ATTTAAAT ATTT^AAAT
402 ATTTAAAT
Van91I 1817 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2650 CCATTTCCTGG
XbaI 2143 TCTAGA T^CTAGA
3418 TCTAGA
XmaCI 56 CCCGGG C^CCGGG
158 CCCGGG
XmaI 56 CCCGGG C^CCGGG
158 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspEI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI Eam1105I EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PshBI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI