##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AccIII AclI AflII AgeI Alw44I
ApaLI AscI AseI AsnI Asp700I
AviII BbeI BcgI BciVI BfrI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KasI KspI MamI
MfeI MroI MunI NarI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspI SwaI Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1487 GACGTC GACGT^C
Acc65I 1386 GGTACC G^GTACC
AccII 2266 CGCG CG^CG
AcyI 1487 GACGTC G[GA]^CG[CT]C
AflIII 2265 ACGCGT A^C[GA][CT]GT
AocI 2511 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2256 AGCGCT AGC^GCT
ApaI 695 GGGCCC GGGCC^C
Asp718I 1386 GGTACC G^GTACC
AspI 4038 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2672 CCTAGG C^CTAGG
BalI 1627 TGGCCA TGG^CCA
BamHI 4072 GGATCC G^GATCC
BcnI 37 CCGGG CC^[GC]GG
BglI 2957 GCCGGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2672 CCTAGG C^CTAGG
BsaHI 1487 GACGTC G[GA]^CG[CT]C
BsmBI 608 CGTCTC CGTCTC(1/5)
BsmI 916 GCATTC GAATGC(1/-1)
BspHI 1973 TCATGA T^CATGA
BstEII 4022 GGTAACC G^GT[ACGT]ACC
BstPI 4022 GGTAACC G^GT[ACGT]ACC
BstUI 2266 CGCG CG^CG
Bsu36I 2511 CCTCAGG CC^T[ACGT]AGG
DraIII 3048 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 677 GACTGTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2256 AGCGCT AGC^GCT
Eco81I 2511 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4022 GGTAACC G^GT[ACGT]ACC
HaeII 2256 AGCGCT [GA]GCGC^[CT]
HgaI 1874 GCGTC GACGC(5/10)
Hin1I 1487 GACGTC G[GA]^CG[CT]C
HindIII 4329 AAGCTT A^AGCTT
KpnI 1386 GGTACC GGTAC^C
MluI 2265 ACGCGT A^CGCGT
MluNI 1627 TGGCCA TGG^CCA
MscI 1627 TGGCCA TGG^CCA
MvnI 2266 CGCG CG^CG
NaeI 4128 GCCGGC GCC^GGC
NciI 37 CCGGG CC^[GC]GG
NcoI 2001 CCATGG C^CATGG
NdeI 3997 CATATG CA^TATG
NgoMIV 4128 GCCGGC G^CCGGC
NheI 3378 GCTAGC G^CTAGC
PflFI 4038 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 550 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2330 CTGCAG CTGCA^G
PvuII 4150 CAGCTG CAG^CTG
RcaI 1973 TCATGA T^CATGA
SpeI 3637 ACTAGT A^CTAGT
SphI 515 GCATGC GCATG^C
Tth111I 4038 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3814 TCTAGA T^CTAGA
XcmI 2223 CCAGAGCTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AhdI 1482 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACTTGAAGTC
AlwNI 528 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
775 CAGCCCCTG
AspEI 1482 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACTTGAAGTC
BbrPI 2909 CACGTG CAC^GTG
4164 CACGTG
BbvCI 1495 CCTCAGC CCTCAGC(-5/-2)
2438 GCTGAGG
BclI 980 TGATCA T^GATCA
2892 TGATCA
BlpI 329 GCTGAGC GC^T[ACGT]AGC
1667 GCTGAGC
Bpu1102I 329 GCTGAGC GC^T[ACGT]AGC
1667 GCTGAGC
BsaAI 2909 CACGTG [CT]AC^GT[GA]
4164 CACGTG
Bsp1407I 2924 TGTACA T^GTACA
4393 TGTACA
BspMI 1464 ACCTGC ACCTGC(4/8)
4124 ACCTGC
BsrBI 706 CCGCTC CCGCTC(-3/-3)
2487 CCGCTC
BsrGI 2924 TGTACA T^GTACA
4393 TGTACA
BstAPI 527 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2409 GCACACGCTGC
CelII 329 GCTGAGC GC^T[ACGT]AGC
1667 GCTGAGC
CfoI 2257 GCGC GCG^C
3067 GCGC
Eam1105I 1482 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3388 GACTTGAAGTC
EcoRI 1276 GAATTC G^AATTC
1703 GAATTC
FbaI 980 TGATCA T^GATCA
2892 TGATCA
HhaI 2257 GCGC GCG^C
3067 GCGC
HinP1I 2257 GCGC G^CGC
3067 GCGC
NspI 515 GCATGC [GA]CATG^[CT]
4371 GCATGT
PmaCI 2909 CACGTG CAC^GTG
4164 CACGTG
PmlI 2909 CACGTG CAC^GTG
4164 CACGTG
SacI 2063 GAGCTC GAGCT^C
4178 GAGCTC
SapI 408 GAAGAGC GCTCTTC(1/4)
1455 GAAGAGC
ScaI 3640 AGTACT AGT^ACT
3988 AGTACT
SexAI 23 ACCAGGT A^CC[AT]GGT
1401 ACCAGGT
SmlI 2340 CTTGAG C^T[CT][GA]AG
3766 CTTGAG
SspBI 2924 TGTACA T^GTACA
4393 TGTACA
StuI 2941 AGGCCT AGG^CCT
3196 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII