################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AhdI Aor51HI AscI Asp718I AspEI AviII BanI BbeI BbrPI BfrI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII BssSI BstBI BstUI CelII ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HaeII HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NotI NruI NspV PacI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 344 GACGCC G[GA]^CG[CT]C AgeI 964 ACCGGT A^CCGGT Alw44I 1773 GTGCAC G^TGCAC ApaI 2264 GGGCCC GGGCC^C ApaLI 1773 GTGCAC G^TGCAC AvrII 2363 CCTAGG C^CTAGG BamHI 1780 GGATCC G^GATCC BbvCI 1559 CCTCAGC CCTCAGC(-5/-2) BcgI 2287 CGAATGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4428 TGATCA T^GATCA BglI 2487 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2363 CCTAGG C^CTAGG BsaHI 344 GACGCC G[GA]^CG[CT]C BsmBI 1308 CGTCTC CGTCTC(1/5) BspLU11I 4447 ACATGT A^CATGT BsrDI 536 CATTGC GCAATG(2/0) Bst1107I 4086 GTATAC GTA^TAC BstAPI 2292 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2007 GGTGACC G^GT[ACGT]ACC BstPI 2007 GGTGACC G^GT[ACGT]ACC BstZ17I 4086 GTATAC GTA^TAC CfoI 1225 GCGC GCG^C DrdI 2010 GACCGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2834 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2007 GGTGACC G^GT[ACGT]ACC FbaI 4428 TGATCA T^GATCA HhaI 1225 GCGC GCG^C Hin1I 344 GACGCC G[GA]^CG[CT]C HinP1I 1225 GCGC G^CGC NdeI 2993 CATATG CA^TATG NheI 1874 GCTAGC G^CTAGC PaeR7I 78 CTCGAG C^TCGAG PinAI 964 ACCGGT A^CCGGT SacI 1023 GAGCTC GAGCT^C SapI 2342 GAAGAGC GCTCTTC(1/4) ScaI 3220 AGTACT AGT^ACT SexAI 3031 ACCTGGT A^CC[AT]GGT SmaI 29 CCCGGG CCC^GGG SnaBI 4089 TACGTA TAC^GTA SpeI 692 ACTAGT A^CTAGT SphI 599 GCATGC GCATG^C XbaI 160 TCTAGA T^CTAGA XhoI 78 CTCGAG C^TCGAG XmaCI 29 CCCGGG C^CCGGG XmaI 29 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 1305 ACACGT A^C[GA][CT]GT 4447 ACATGT AlwNI 1044 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 2293 CAGTGCCTG AseI 3760 ATTAAT AT^TAAT 3908 ATTAAT AsnI 3760 ATTAAT AT^TAAT 3908 ATTAAT AspI 2391 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4179 GACAAAGTC AvaI 29 CCCGGG C^[CT]CG[GA]G 78 CTCGAG BcnI 29 CCCGG CC^[GC]GG 30 CCGGG BsaAI 706 TACGTG [CT]AC^GT[GA] 4089 TACGTA BsoBI 29 CCCGGG C^[CT]CG[GA]G 78 CTCGAG BspHI 424 TCATGA T^CATGA 2750 TCATGA DraI 3263 TTTAAA TTT^AAA 3822 TTTAAA EcoT22I 597 ATGCAT ATGCA^T 2565 ATGCAT HgaI 304 GACGC GACGC(5/10) 344 GACGC HpaI 696 GTTAAC GTT^AAC 3645 GTTAAC NciI 29 CCCGG CC^[GC]GG 30 CCGGG NsiI 597 ATGCAT ATGCA^T 2565 ATGCAT PflFI 2391 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4179 GACAAAGTC PpuMI 1113 GGGTCCC [GA]G^G[AT]CC[CT] 2651 GGGACCC PshBI 3760 ATTAAT AT^TAAT 3908 ATTAAT RcaI 424 TCATGA T^CATGA 2750 TCATGA StuI 850 AGGCCT AGG^CCT 1808 AGGCCT Tth111I 2391 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 4179 GACAAAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AocI ApoI Asp700I AspHI AvaII BalI BanII BbsI BbvI BciVI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI