##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AhdI Aor51HI AscI Asp718I
AspEI AviII BanI BbeI BbrPI
BfrI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BssHII BssSI BstBI BstUI CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI HaeII HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NarI NotI NruI
NspV PacI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 344 GACGCC G[GA]^CG[CT]C
AgeI 964 ACCGGT A^CCGGT
Alw44I 1773 GTGCAC G^TGCAC
ApaI 2264 GGGCCC GGGCC^C
ApaLI 1773 GTGCAC G^TGCAC
AvrII 2363 CCTAGG C^CTAGG
BamHI 1780 GGATCC G^GATCC
BbvCI 1559 CCTCAGC CCTCAGC(-5/-2)
BcgI 2287 CGAATGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4428 TGATCA T^GATCA
BglI 2487 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2363 CCTAGG C^CTAGG
BsaHI 344 GACGCC G[GA]^CG[CT]C
BsmBI 1308 CGTCTC CGTCTC(1/5)
BspLU11I 4447 ACATGT A^CATGT
BsrDI 536 CATTGC GCAATG(2/0)
Bst1107I 4086 GTATAC GTA^TAC
BstAPI 2292 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2007 GGTGACC G^GT[ACGT]ACC
BstPI 2007 GGTGACC G^GT[ACGT]ACC
BstZ17I 4086 GTATAC GTA^TAC
CfoI 1225 GCGC GCG^C
DrdI 2010 GACCGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2834 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2007 GGTGACC G^GT[ACGT]ACC
FbaI 4428 TGATCA T^GATCA
HhaI 1225 GCGC GCG^C
Hin1I 344 GACGCC G[GA]^CG[CT]C
HinP1I 1225 GCGC G^CGC
NdeI 2993 CATATG CA^TATG
NheI 1874 GCTAGC G^CTAGC
PaeR7I 78 CTCGAG C^TCGAG
PinAI 964 ACCGGT A^CCGGT
SacI 1023 GAGCTC GAGCT^C
SapI 2342 GAAGAGC GCTCTTC(1/4)
ScaI 3220 AGTACT AGT^ACT
SexAI 3031 ACCTGGT A^CC[AT]GGT
SmaI 29 CCCGGG CCC^GGG
SnaBI 4089 TACGTA TAC^GTA
SpeI 692 ACTAGT A^CTAGT
SphI 599 GCATGC GCATG^C
XbaI 160 TCTAGA T^CTAGA
XhoI 78 CTCGAG C^TCGAG
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1305 ACACGT A^C[GA][CT]GT
4447 ACATGT
AlwNI 1044 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2293 CAGTGCCTG
AseI 3760 ATTAAT AT^TAAT
3908 ATTAAT
AsnI 3760 ATTAAT AT^TAAT
3908 ATTAAT
AspI 2391 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4179 GACAAAGTC
AvaI 29 CCCGGG C^[CT]CG[GA]G
78 CTCGAG
BcnI 29 CCCGG CC^[GC]GG
30 CCGGG
BsaAI 706 TACGTG [CT]AC^GT[GA]
4089 TACGTA
BsoBI 29 CCCGGG C^[CT]CG[GA]G
78 CTCGAG
BspHI 424 TCATGA T^CATGA
2750 TCATGA
DraI 3263 TTTAAA TTT^AAA
3822 TTTAAA
EcoT22I 597 ATGCAT ATGCA^T
2565 ATGCAT
HgaI 304 GACGC GACGC(5/10)
344 GACGC
HpaI 696 GTTAAC GTT^AAC
3645 GTTAAC
NciI 29 CCCGG CC^[GC]GG
30 CCGGG
NsiI 597 ATGCAT ATGCA^T
2565 ATGCAT
PflFI 2391 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4179 GACAAAGTC
PpuMI 1113 GGGTCCC [GA]G^G[AT]CC[CT]
2651 GGGACCC
PshBI 3760 ATTAAT AT^TAAT
3908 ATTAAT
RcaI 424 TCATGA T^CATGA
2750 TCATGA
StuI 850 AGGCCT AGG^CCT
1808 AGGCCT
Tth111I 2391 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
4179 GACAAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI Asp700I AspHI
AvaII BalI BanII BbsI BbvI
BciVI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI