##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII BbrPI BcgI BciVI
BclI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrGI Bst1107I BstBI
BstZ17I CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SpeI Sse8387I SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 1385 TCCGGA T^CCGGA
AcsI 3494 AAATTC [GA]^AATT[CT]
AflII 802 CTTAAG C^TTAAG
AflIII 2476 ACGTGT A^C[GA][CT]GT
Alw44I 1021 GTGCAC G^TGCAC
AocI 2640 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1352 AGCGCT AGC^GCT
ApaLI 1021 GTGCAC G^TGCAC
ApoI 3494 AAATTC [GA]^AATT[CT]
Asp700I 4093 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1315 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2457 CCTAGG C^CTAGG
BfrI 802 CTTAAG C^TTAAG
BglII 4296 AGATCT A^GATCT
BlnI 2457 CCTAGG C^CTAGG
BlpI 2206 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2206 GCTCAGC GC^T[ACGT]AGC
BseAI 1385 TCCGGA T^CCGGA
BsiEI 1 CGGCCG CG[GA][CT]^CG
BspDI 1030 ATCGAT AT^CGAT
BspEI 1385 TCCGGA T^CCGGA
BsrDI 533 GCAATG GCAATG(2/0)
BssHII 2081 GCGCGC G^CGCGC
BssSI 4117 CTCGTG CACGAG(-5/-1)
BstEII 2613 GGTCACC G^GT[ACGT]ACC
BstPI 2613 GGTCACC G^GT[ACGT]ACC
Bsu36I 2640 CCTCAGG CC^T[ACGT]AGG
CelII 2206 GCTCAGC GC^T[ACGT]AGC
ClaI 1030 ATCGAT AT^CGAT
DrdI 273 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1 CGGCCG C^GGCCG
EclXI 1 CGGCCG C^GGCCG
Eco47III 1352 AGCGCT AGC^GCT
Eco52I 1 CGGCCG C^GGCCG
Eco81I 2640 CCTCAGG CC^T[ACGT]AGG
EcoNI 171 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2613 GGTCACC G^GT[ACGT]ACC
MroI 1385 TCCGGA T^CCGGA
NheI 3503 GCTAGC G^CTAGC
PflFI 1315 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 861 GAAGAGC GCTCTTC(1/4)
SfiI 1720 GGCCAGGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 562 CCCGGG CCC^GGG
SnaBI 261 TACGTA TAC^GTA
Tth111I 1315 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2405 TCTAGA T^CTAGA
XmaCI 562 CCCGGG C^CCGGG
XmaI 562 CCCGGG C^CCGGG
XmnI 4093 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
BamHI 1190 GGATCC G^GATCC
4144 GGATCC
BsaI 3528 GAGACC GGTCTC(1/5)
3738 GAGACC
BsaWI 1385 TCCGGA [AT]^CCGG[AT]
4038 TCCGGT
BspHI 764 TCATGA T^CATGA
1367 TCATGA
BsrBI 349 GAGCGG CCGCTC(-3/-3)
1660 GAGCGG
DraIII 3054 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3564 CACTCAGTG
FseI 1561 GGCCGGCC GGCCGG^CC
1729 GGCCGGCC
RcaI 764 TCATGA T^CATGA
1367 TCATGA
SphI 2674 GCATGC GCATG^C
3685 GCATGC
Tsp509I 3384 AATT ^AATT
3495 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII