##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccII AclI AflII AscI
AseI AsnI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BlnI BsaBI BsiWI BspDI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DraIII DrdI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI KspI MamI MluI MvnI
NarI NdeI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 1394 TCCGGA T^CCGGA
AcyI 1574 GGCGTC G[GA]^CG[CT]C
AgeI 539 ACCGGT A^CCGGT
Alw44I 3227 GTGCAC G^TGCAC
Aor51HI 1604 AGCGCT AGC^GCT
ApaI 1438 GGGCCC GGGCC^C
ApaLI 3227 GTGCAC G^TGCAC
BglII 3422 AGATCT A^GATCT
BsaAI 2321 TACGTG [CT]AC^GT[GA]
BsaHI 1574 GGCGTC G[GA]^CG[CT]C
BseAI 1394 TCCGGA T^CCGGA
BsiEI 1139 CGGCCG CG[GA][CT]^CG
Bsp1407I 4125 TGTACA T^GTACA
BspEI 1394 TCCGGA T^CCGGA
BspLU11I 2463 ACATGT A^CATGT
BsrGI 4125 TGTACA T^GTACA
BssSI 3590 CTCGTG CACGAG(-5/-1)
EagI 1139 CGGCCG C^GGCCG
EclXI 1139 CGGCCG C^GGCCG
Eco47III 1604 AGCGCT AGC^GCT
Eco52I 1139 CGGCCG C^GGCCG
HgaI 1575 GCGTC GACGC(5/10)
Hin1I 1574 GGCGTC G[GA]^CG[CT]C
HincII 894 GTCAAC GT[CT]^[GA]AC
HindII 894 GTCAAC GT[CT]^[GA]AC
HindIII 2392 AAGCTT A^AGCTT
MroI 1394 TCCGGA T^CCGGA
NaeI 2666 GCCGGC GCC^GGC
NgoMIV 2666 GCCGGC G^CCGGC
PinAI 539 ACCGGT A^CCGGT
PshAI 839 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 109 CCTGCAGG CCTGCA^GG
SphI 1649 GCATGC GCATG^C
Sse8387I 109 CCTGCAGG CCTGCA^GG
SspBI 4125 TGTACA T^GTACA
SspI 193 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 2827 GAATTT [GA]^AATT[CT]
3457 AAATTC
AflIII 2322 ACGTGT A^C[GA][CT]GT
2463 ACATGT
AhdI 175 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
429 GACTGAGAGTC
ApoI 2827 GAATTT [GA]^AATT[CT]
3457 AAATTC
Asp700I 1728 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4211 GAAGAGCTTC
AspEI 175 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
429 GACTGAGAGTC
AspI 879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3360 GACCCTGTC
BalI 144 TGGCCA TGG^CCA
2231 TGGCCA
BbsI 2242 GAAGAC GAAGAC(2/6)
1134 GTCTTC
BbvCI 3087 CCTCAGC CCTCAGC(-5/-2)
3131 CCTCAGC
BglI 2071 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2263 GCCATGGTGGC
BpuAI 2242 GAAGAC GAAGAC(2/6)
1134 GTCTTC
BsaWI 539 ACCGGT [AT]^CCGG[AT]
1394 TCCGGA
BsmBI 2775 CGTCTC CGTCTC(1/5)
1855 GAGACG
Eam1105I 175 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
429 GACTGAGAGTC
HaeII 1604 AGCGCT [GA]GCGC^[CT]
2108 AGCGCC
MfeI 387 CAATTG C^AATTG
4059 CAATTG
MluNI 144 TGGCCA TGG^CCA
2231 TGGCCA
MscI 144 TGGCCA TGG^CCA
2231 TGGCCA
MunI 387 CAATTG C^AATTG
4059 CAATTG
NheI 552 GCTAGC G^CTAGC
1806 GCTAGC
PflFI 879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3360 GACCCTGTC
SapI 1453 GAAGAGC GCTCTTC(1/4)
4211 GAAGAGC
TfiI 1219 GAATC G^A[AT]TC
3306 GAATC
Tth111I 879 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3360 GACCCTGTC
XcmI 734 CCATAGGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1748 CCAATGAGAACATGG
XmnI 1728 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4211 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
Acc65I AccI AciI AfaI AluI
AlwI AlwNI AocI Asp718I AspHI
AvaI AvaII BanI BanII BbvI
BcgI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI