##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AscI Asp718I AspEI
AspI BcgI BsaBI BseAI BsgI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstZ17I Cfr10I ClaI CpoI Eam1105I
EcoRV EcoT22I FseI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NruI
NsiI PacI PflFI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SfiI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 4115 CTTAAG C^TTAAG
AflIII 2095 ACACGT A^C[GA][CT]GT
Alw44I 4468 GTGCAC G^TGCAC
ApaLI 4468 GTGCAC G^TGCAC
AseI 189 ATTAAT AT^TAAT
AsnI 189 ATTAAT AT^TAAT
Asp700I 1611 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2995 TGCGCA TGC^GCA
BbeI 879 GGCGCC GGCGC^C
BbrPI 2096 CACGTG CAC^GTG
BclI 4716 TGATCA T^GATCA
BfrI 4115 CTTAAG C^TTAAG
BglII 4499 AGATCT A^GATCT
BsiEI 2918 CGGCCG CG[GA][CT]^CG
BsiWI 604 CGTACG C^GTACG
BsmI 3361 GAATGC GAATGC(1/-1)
BssSI 1797 CTCGTG CACGAG(-5/-1)
BstBI 4449 TTCGAA TT^CGAA
BstEII 2771 GGTCACC G^GT[ACGT]ACC
BstPI 2771 GGTCACC G^GT[ACGT]ACC
DraIII 1360 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4818 GACAGAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2918 CGGCCG C^GGCCG
EclXI 2918 CGGCCG C^GGCCG
Eco52I 2918 CGGCCG C^GGCCG
EcoO65I 2771 GGTCACC G^GT[ACGT]ACC
EcoRI 3200 GAATTC G^AATTC
FbaI 4716 TGATCA T^GATCA
FspI 2995 TGCGCA TGC^GCA
KasI 879 GGCGCC G^GCGCC
NarI 879 GGCGCC GG^CGCC
NdeI 1780 CATATG CA^TATG
NotI 2917 GCGGCCGC GC^GGCCGC
NspV 4449 TTCGAA TT^CGAA
PaeR7I 460 CTCGAG C^TCGAG
PmaCI 2096 CACGTG CAC^GTG
PmlI 2096 CACGTG CAC^GTG
PshAI 1778 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 189 ATTAAT AT^TAAT
SbfI 2639 CCTGCAGG CCTGCA^GG
SfoI 879 GGCGCC GGC^GCC
SfuI 4449 TTCGAA TT^CGAA
SmaI 856 CCCGGG CCC^GGG
Sse8387I 2639 CCTGCAGG CCTGCA^GG
XcmI 1625 CCACATCAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 460 CTCGAG C^TCGAG
XmaCI 856 CCCGGG C^CCGGG
XmaI 856 CCCGGG C^CCGGG
XmnI 1611 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 879 GGCGCC G[GA]^CG[CT]C
1638 GGCGTC
AocI 847 CCTCAGG CC^T[ACGT]AGG
1386 CCTGAGG
AspHI 585 GAGCAC G[AT]GC[AT]^C
4468 GTGCAC
AvrII 596 CCTAGG C^CTAGG
4271 CCTAGG
BanI 879 GGCGCC G^G[CT][GA]CC
3467 GGTGCC
BciVI 2525 GGATAC GTATCC(6/5)
4852 GTATCC
BglI 2337 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2583 GCCCGTGGGGC
BlnI 596 CCTAGG C^CTAGG
4271 CCTAGG
BlpI 1431 GCTGAGC GC^T[ACGT]AGC
3376 GCTCAGC
Bpu1102I 1431 GCTGAGC GC^T[ACGT]AGC
3376 GCTCAGC
BsaAI 606 TACGTG [CT]AC^GT[GA]
2096 CACGTG
BsaHI 879 GGCGCC G[GA]^CG[CT]C
1638 GGCGTC
BsaWI 2038 ACCGGA [AT]^CCGG[AT]
2786 ACCGGA
BsiHKAI 585 GAGCAC G[AT]GC[AT]^C
4468 GTGCAC
Bsu36I 847 CCTCAGG CC^T[ACGT]AGG
1386 CCTGAGG
CelII 1431 GCTGAGC GC^T[ACGT]AGC
3376 GCTCAGC
Eco81I 847 CCTCAGG CC^T[ACGT]AGG
1386 CCTGAGG
Hin1I 879 GGCGCC G[GA]^CG[CT]C
1638 GGCGTC
HincII 1676 GTTGAC GT[CT]^[GA]AC
4416 GTCAAC
HindII 1676 GTTGAC GT[CT]^[GA]AC
4416 GTCAAC
HindIII 221 AAGCTT A^AGCTT
1458 AAGCTT
NcoI 1396 CCATGG C^CATGG
2341 CCATGG
PflMI 1329 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3420 CCACATGATGG
SexAI 1576 ACCTGGT A^CC[AT]GGT
4703 ACCTGGT
SphI 163 GCATGC GCATG^C
2701 GCATGC
Van91I 1329 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3420 CCACATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI Aor51HI ApaI ApoI
AvaI AvaII BalI BamHI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII