##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        AhdI        AscI        Asp718I     AspEI       
AspI        BcgI        BsaBI       BseAI       BsgI        
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      Bst1107I    
BstZ17I     Cfr10I      ClaI        CpoI        Eam1105I    
EcoRV       EcoT22I     FseI        HpaI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NgoMIV      NheI        NruI        
NsiI        PacI        PflFI       PinAI       PmeI        
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        ScaI        SfiI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflII        4115  CTTAAG               C^TTAAG
AflIII       2095  ACACGT               A^C[GA][CT]GT
Alw44I       4468  GTGCAC               G^TGCAC
ApaLI        4468  GTGCAC               G^TGCAC
AseI          189  ATTAAT               AT^TAAT
AsnI          189  ATTAAT               AT^TAAT
Asp700I      1611  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        2995  TGCGCA               TGC^GCA
BbeI          879  GGCGCC               GGCGC^C
BbrPI        2096  CACGTG               CAC^GTG
BclI         4716  TGATCA               T^GATCA
BfrI         4115  CTTAAG               C^TTAAG
BglII        4499  AGATCT               A^GATCT
BsiEI        2918  CGGCCG               CG[GA][CT]^CG
BsiWI         604  CGTACG               C^GTACG
BsmI         3361  GAATGC               GAATGC(1/-1)
BssSI        1797  CTCGTG               CACGAG(-5/-1)
BstBI        4449  TTCGAA               TT^CGAA
BstEII       2771  GGTCACC              G^GT[ACGT]ACC
BstPI        2771  GGTCACC              G^GT[ACGT]ACC
DraIII       1360  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4818  GACAGAACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2918  CGGCCG               C^GGCCG
EclXI        2918  CGGCCG               C^GGCCG
Eco52I       2918  CGGCCG               C^GGCCG
EcoO65I      2771  GGTCACC              G^GT[ACGT]ACC
EcoRI        3200  GAATTC               G^AATTC
FbaI         4716  TGATCA               T^GATCA
FspI         2995  TGCGCA               TGC^GCA
KasI          879  GGCGCC               G^GCGCC
NarI          879  GGCGCC               GG^CGCC
NdeI         1780  CATATG               CA^TATG
NotI         2917  GCGGCCGC             GC^GGCCGC
NspV         4449  TTCGAA               TT^CGAA
PaeR7I        460  CTCGAG               C^TCGAG
PmaCI        2096  CACGTG               CAC^GTG
PmlI         2096  CACGTG               CAC^GTG
PshAI        1778  GACATATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         189  ATTAAT               AT^TAAT
SbfI         2639  CCTGCAGG             CCTGCA^GG
SfoI          879  GGCGCC               GGC^GCC
SfuI         4449  TTCGAA               TT^CGAA
SmaI          856  CCCGGG               CCC^GGG
Sse8387I     2639  CCTGCAGG             CCTGCA^GG
XcmI         1625  CCACATCAAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          460  CTCGAG               C^TCGAG
XmaCI         856  CCCGGG               C^CCGGG
XmaI          856  CCCGGG               C^CCGGG
XmnI         1611  GAATGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          879  GGCGCC               G[GA]^CG[CT]C
             1638  GGCGTC              
AocI          847  CCTCAGG              CC^T[ACGT]AGG
             1386  CCTGAGG             
AspHI         585  GAGCAC               G[AT]GC[AT]^C
             4468  GTGCAC              
AvrII         596  CCTAGG               C^CTAGG
             4271  CCTAGG              
BanI          879  GGCGCC               G^G[CT][GA]CC
             3467  GGTGCC              
BciVI        2525  GGATAC               GTATCC(6/5)
             4852  GTATCC              
BglI         2337  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2583  GCCCGTGGGGC         
BlnI          596  CCTAGG               C^CTAGG
             4271  CCTAGG              
BlpI         1431  GCTGAGC              GC^T[ACGT]AGC
             3376  GCTCAGC             
Bpu1102I     1431  GCTGAGC              GC^T[ACGT]AGC
             3376  GCTCAGC             
BsaAI         606  TACGTG               [CT]AC^GT[GA]
             2096  CACGTG              
BsaHI         879  GGCGCC               G[GA]^CG[CT]C
             1638  GGCGTC              
BsaWI        2038  ACCGGA               [AT]^CCGG[AT]
             2786  ACCGGA              
BsiHKAI       585  GAGCAC               G[AT]GC[AT]^C
             4468  GTGCAC              
Bsu36I        847  CCTCAGG              CC^T[ACGT]AGG
             1386  CCTGAGG             
CelII        1431  GCTGAGC              GC^T[ACGT]AGC
             3376  GCTCAGC             
Eco81I        847  CCTCAGG              CC^T[ACGT]AGG
             1386  CCTGAGG             
Hin1I         879  GGCGCC               G[GA]^CG[CT]C
             1638  GGCGTC              
HincII       1676  GTTGAC               GT[CT]^[GA]AC
             4416  GTCAAC              
HindII       1676  GTTGAC               GT[CT]^[GA]AC
             4416  GTCAAC              
HindIII       221  AAGCTT               A^AGCTT
             1458  AAGCTT              
NcoI         1396  CCATGG               C^CATGG
             2341  CCATGG              
PflMI        1329  CCACATAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3420  CCACATGATGG         
SexAI        1576  ACCTGGT              A^CC[AT]GGT
             4703  ACCTGGT             
SphI          163  GCATGC               GCATG^C
             2701  GCATGC              
Van91I       1329  CCACATAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3420  CCACATGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        ApoI        
AvaI        AvaII       BalI        BamHI       BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        Eco47III    EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII