################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI AscI Asp718I AspEI AspI BcgI BsaBI BseAI BsgI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstZ17I Cfr10I ClaI CpoI Eam1105I EcoRV EcoT22I FseI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NgoMIV NheI NruI NsiI PacI PflFI PinAI PmeI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SfiI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflII 4115 CTTAAG C^TTAAG AflIII 2095 ACACGT A^C[GA][CT]GT Alw44I 4468 GTGCAC G^TGCAC ApaLI 4468 GTGCAC G^TGCAC AseI 189 ATTAAT AT^TAAT AsnI 189 ATTAAT AT^TAAT Asp700I 1611 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2995 TGCGCA TGC^GCA BbeI 879 GGCGCC GGCGC^C BbrPI 2096 CACGTG CAC^GTG BclI 4716 TGATCA T^GATCA BfrI 4115 CTTAAG C^TTAAG BglII 4499 AGATCT A^GATCT BsiEI 2918 CGGCCG CG[GA][CT]^CG BsiWI 604 CGTACG C^GTACG BsmI 3361 GAATGC GAATGC(1/-1) BssSI 1797 CTCGTG CACGAG(-5/-1) BstBI 4449 TTCGAA TT^CGAA BstEII 2771 GGTCACC G^GT[ACGT]ACC BstPI 2771 GGTCACC G^GT[ACGT]ACC DraIII 1360 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4818 GACAGAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2918 CGGCCG C^GGCCG EclXI 2918 CGGCCG C^GGCCG Eco52I 2918 CGGCCG C^GGCCG EcoO65I 2771 GGTCACC G^GT[ACGT]ACC EcoRI 3200 GAATTC G^AATTC FbaI 4716 TGATCA T^GATCA FspI 2995 TGCGCA TGC^GCA KasI 879 GGCGCC G^GCGCC NarI 879 GGCGCC GG^CGCC NdeI 1780 CATATG CA^TATG NotI 2917 GCGGCCGC GC^GGCCGC NspV 4449 TTCGAA TT^CGAA PaeR7I 460 CTCGAG C^TCGAG PmaCI 2096 CACGTG CAC^GTG PmlI 2096 CACGTG CAC^GTG PshAI 1778 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 189 ATTAAT AT^TAAT SbfI 2639 CCTGCAGG CCTGCA^GG SfoI 879 GGCGCC GGC^GCC SfuI 4449 TTCGAA TT^CGAA SmaI 856 CCCGGG CCC^GGG Sse8387I 2639 CCTGCAGG CCTGCA^GG XcmI 1625 CCACATCAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 460 CTCGAG C^TCGAG XmaCI 856 CCCGGG C^CCGGG XmaI 856 CCCGGG C^CCGGG XmnI 1611 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 879 GGCGCC G[GA]^CG[CT]C 1638 GGCGTC AocI 847 CCTCAGG CC^T[ACGT]AGG 1386 CCTGAGG AspHI 585 GAGCAC G[AT]GC[AT]^C 4468 GTGCAC AvrII 596 CCTAGG C^CTAGG 4271 CCTAGG BanI 879 GGCGCC G^G[CT][GA]CC 3467 GGTGCC BciVI 2525 GGATAC GTATCC(6/5) 4852 GTATCC BglI 2337 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2583 GCCCGTGGGGC BlnI 596 CCTAGG C^CTAGG 4271 CCTAGG BlpI 1431 GCTGAGC GC^T[ACGT]AGC 3376 GCTCAGC Bpu1102I 1431 GCTGAGC GC^T[ACGT]AGC 3376 GCTCAGC BsaAI 606 TACGTG [CT]AC^GT[GA] 2096 CACGTG BsaHI 879 GGCGCC G[GA]^CG[CT]C 1638 GGCGTC BsaWI 2038 ACCGGA [AT]^CCGG[AT] 2786 ACCGGA BsiHKAI 585 GAGCAC G[AT]GC[AT]^C 4468 GTGCAC Bsu36I 847 CCTCAGG CC^T[ACGT]AGG 1386 CCTGAGG CelII 1431 GCTGAGC GC^T[ACGT]AGC 3376 GCTCAGC Eco81I 847 CCTCAGG CC^T[ACGT]AGG 1386 CCTGAGG Hin1I 879 GGCGCC G[GA]^CG[CT]C 1638 GGCGTC HincII 1676 GTTGAC GT[CT]^[GA]AC 4416 GTCAAC HindII 1676 GTTGAC GT[CT]^[GA]AC 4416 GTCAAC HindIII 221 AAGCTT A^AGCTT 1458 AAGCTT NcoI 1396 CCATGG C^CATGG 2341 CCATGG PflMI 1329 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3420 CCACATGATGG SexAI 1576 ACCTGGT A^CC[AT]GGT 4703 ACCTGGT SphI 163 GCATGC GCATG^C 2701 GCATGC Van91I 1329 CCACATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3420 CCACATGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI Aor51HI ApaI ApoI AvaI AvaII BalI BamHI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII