##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AseI AsnI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BlnI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EcoO65I EcoRV EcoT22I FseI HindIII
HpaI KasI KspI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 4138 GGTACC G^GTACC
AccII 1411 CGCG CG^CG
AcyI 2566 GGCGTC G[GA]^CG[CT]C
AhdI 720 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4094 GTGCAC G^TGCAC
AocI 3471 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3770 AGCGCT AGC^GCT
ApaI 2717 GGGCCC GGGCC^C
ApaLI 4094 GTGCAC G^TGCAC
Asp718I 4138 GGTACC G^GTACC
AspEI 720 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3189 CTCGGG C^[CT]CG[GA]G
AviII 3004 TGCGCA TGC^GCA
BbsI 4208 GTCTTC GAAGAC(2/6)
BcnI 943 CCCGG CC^[GC]GG
BglI 91 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 700 AGATCT A^GATCT
BlpI 1275 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1275 GCTGAGC GC^T[ACGT]AGC
BpuAI 4208 GTCTTC GAAGAC(2/6)
BsaAI 3735 CACGTA [CT]AC^GT[GA]
BsaBI 662 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2566 GGCGTC G[GA]^CG[CT]C
BsaI 3281 GGTCTC GGTCTC(1/5)
BsiEI 76 CGGCCG CG[GA][CT]^CG
BsmBI 2568 CGTCTC CGTCTC(1/5)
BsoBI 3189 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4080 TGTACA T^GTACA
BsrGI 4080 TGTACA T^GTACA
BssSI 1577 CTCGTG CACGAG(-5/-1)
BstUI 1411 CGCG CG^CG
Bsu36I 3471 CCTTAGG CC^T[ACGT]AGG
CelII 1275 GCTGAGC GC^T[ACGT]AGC
EagI 76 CGGCCG C^GGCCG
Eam1105I 720 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 76 CGGCCG C^GGCCG
Eco47III 3770 AGCGCT AGC^GCT
Eco52I 76 CGGCCG C^GGCCG
Eco81I 3471 CCTTAGG CC^T[ACGT]AGG
EcoNI 455 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3004 TGCGCA TGC^GCA
HaeII 3770 AGCGCT [GA]GCGC^[CT]
HapII 944 CCGG C^CGG
Hin1I 2566 GGCGTC G[GA]^CG[CT]C
HincII 909 GTCAAC GT[CT]^[GA]AC
HindII 909 GTCAAC GT[CT]^[GA]AC
HpaII 944 CCGG C^CGG
KpnI 4138 GGTACC GGTAC^C
MamI 662 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 944 CCGG C^CGG
MvnI 1411 CGCG CG^CG
NciI 943 CCCGG CC^[GC]GG
NheI 3364 GCTAGC G^CTAGC
SspBI 4080 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1406 GTAGAC GT^[AC][GT]AC
2929 GTCTAC
AflII 552 CTTAAG C^TTAAG
4381 CTTAAG
AlwNI 1317 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
4046 CAGATCCTG
BalI 3058 TGGCCA TGG^CCA
3065 TGGCCA
BciVI 245 GGATAC GTATCC(6/5)
833 GTATCC
BfrI 552 CTTAAG C^TTAAG
4381 CTTAAG
BsmI 755 GAATGC GAATGC(1/-1)
4437 GCATTC
BspLU11I 131 ACATGT A^CATGT
1674 ACATGT
DsaI 2596 CCATGG C^C[GA][CT]GG
3312 CCATGG
EcoRI 426 GAATTC G^AATTC
1725 GAATTC
HgaI 1409 GACGC GACGC(5/10)
2567 GCGTC
MluNI 3058 TGGCCA TGG^CCA
3065 TGGCCA
MscI 3058 TGGCCA TGG^CCA
3065 TGGCCA
NcoI 2596 CCATGG C^CATGG
3312 CCATGG
NdeI 1036 CATATG CA^TATG
1631 CATATG
SacI 1068 GAGCTC GAGCT^C
1134 GAGCTC
ScaI 482 AGTACT AGT^ACT
3536 AGTACT
SexAI 3080 ACCAGGT A^CC[AT]GGT
4407 ACCTGGT
SphI 1367 GCATGC GCATG^C
2157 GCATGC
TaqI 110 TCGA T^CGA
142 TCGA
TthHB8I 110 TCGA T^CGA
142 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbvI BclI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmnI